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The effect of neonatal capsaicin treatment on the CGRP-immunoreaction in the trigeminal subnucleus caudalis of mice.
Biomed Res. 2008 Feb; 29(1):33-42.BR

Abstract

The calcitonin-gene related peptide (CGRP) is a primary afferent neurotransmitter in the trigeminal system. Although a neonatal administration of capsaicin eliminates substance P (SP)-mediated nociceptive responses to induce a permanent functional reduction in C-fibers, little information is available regarding changes in CGRP-immunoreaction in mice undergoing neonatal capsaicin treatment (CP mice). This study examined postnatal changes in the distribution of CGRP-immunoreaction in the trigeminal subnucleus caudalis and trigeminal ganglion of CP mice by immunohistochemical technique and a quantitative analysis. Immunohistochemistry for CGRP in the subnucleus caudalis (Vc) demonstrated two dense distributions of neurons in the CP mice as well as naïve mice: in the marginal layer and the region 400-600 mum deep. The quantitative analysis revealed no significant difference in the density of CGRP immunoreaction between naïve and CP mice 1-8 weeks of age. In the trigeminal ganglion of both groups, the size distribution of CGRPpositive neurons displayed a distribution pattern with one peak in 200-300 mum(2) at week 1 and with two peaks in 200-300 mum(2) and 600-700 mum(2) at week 8 but no significant difference in neural density existed between these regions. When double staining in the naïve mice with CGRP or SP and VR1, a capsaicin receptor, was done, many trigeminal ganglion neurons co-expressed SP- and VR1-immunoreactions, but rarely exhibited CGRP/VR1-co-localization. Taken together with previous data, these current observations suggest that CGRP containing afferent neurons possibly performs differing roles in nociceptive afferent input transmission within the Vc from SP-containing neurons in mice.

Authors+Show Affiliations

Division of Oral Anatomy, Niigata University Graduate School of Medical and Dental Sciences, Nigata, Japan.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18344596

Citation

Aita, Megumi, et al. "The Effect of Neonatal Capsaicin Treatment On the CGRP-immunoreaction in the Trigeminal Subnucleus Caudalis of Mice." Biomedical Research (Tokyo, Japan), vol. 29, no. 1, 2008, pp. 33-42.
Aita M, Maeda T, Seo K. The effect of neonatal capsaicin treatment on the CGRP-immunoreaction in the trigeminal subnucleus caudalis of mice. Biomed Res. 2008;29(1):33-42.
Aita, M., Maeda, T., & Seo, K. (2008). The effect of neonatal capsaicin treatment on the CGRP-immunoreaction in the trigeminal subnucleus caudalis of mice. Biomedical Research (Tokyo, Japan), 29(1), 33-42.
Aita M, Maeda T, Seo K. The Effect of Neonatal Capsaicin Treatment On the CGRP-immunoreaction in the Trigeminal Subnucleus Caudalis of Mice. Biomed Res. 2008;29(1):33-42. PubMed PMID: 18344596.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The effect of neonatal capsaicin treatment on the CGRP-immunoreaction in the trigeminal subnucleus caudalis of mice. AU - Aita,Megumi, AU - Maeda,Takeyasu, AU - Seo,Kenji, PY - 2008/3/18/pubmed PY - 2008/7/30/medline PY - 2008/3/18/entrez SP - 33 EP - 42 JF - Biomedical research (Tokyo, Japan) JO - Biomed. Res. VL - 29 IS - 1 N2 - The calcitonin-gene related peptide (CGRP) is a primary afferent neurotransmitter in the trigeminal system. Although a neonatal administration of capsaicin eliminates substance P (SP)-mediated nociceptive responses to induce a permanent functional reduction in C-fibers, little information is available regarding changes in CGRP-immunoreaction in mice undergoing neonatal capsaicin treatment (CP mice). This study examined postnatal changes in the distribution of CGRP-immunoreaction in the trigeminal subnucleus caudalis and trigeminal ganglion of CP mice by immunohistochemical technique and a quantitative analysis. Immunohistochemistry for CGRP in the subnucleus caudalis (Vc) demonstrated two dense distributions of neurons in the CP mice as well as naïve mice: in the marginal layer and the region 400-600 mum deep. The quantitative analysis revealed no significant difference in the density of CGRP immunoreaction between naïve and CP mice 1-8 weeks of age. In the trigeminal ganglion of both groups, the size distribution of CGRPpositive neurons displayed a distribution pattern with one peak in 200-300 mum(2) at week 1 and with two peaks in 200-300 mum(2) and 600-700 mum(2) at week 8 but no significant difference in neural density existed between these regions. When double staining in the naïve mice with CGRP or SP and VR1, a capsaicin receptor, was done, many trigeminal ganglion neurons co-expressed SP- and VR1-immunoreactions, but rarely exhibited CGRP/VR1-co-localization. Taken together with previous data, these current observations suggest that CGRP containing afferent neurons possibly performs differing roles in nociceptive afferent input transmission within the Vc from SP-containing neurons in mice. SN - 1880-313X UR - https://www.unboundmedicine.com/medline/citation/18344596/The_effect_of_neonatal_capsaicin_treatment_on_the_CGRP_immunoreaction_in_the_trigeminal_subnucleus_caudalis_of_mice_ L2 - http://japanlinkcenter.org/JST.JSTAGE/biomedres/29.33?from=PubMed DB - PRIME DP - Unbound Medicine ER -