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Candida albicans cell wall comprises a branched beta-D-(1-->6)-glucan with beta-D-(1-->3)-side chains.
Carbohydr Res. 2008 May 05; 343(6):1050-61.CR

Abstract

The structure of immunogenic and immunomodulatory cell wall glucans of Candida albicans is commonly interpreted in terms of a basic polysaccharide consisting of a beta-D-(1-->3)-linked glucopyranosyl backbone possessing beta-D-(1-->6)-linked side chains of varying distribution and length. This proposed molecular architecture has been re-evaluated by the present study on the products of selective enzymolysis of insoluble C. albicans glucan particles (GG). High resolution 1H (400 and 700 MHz) and 13C (100 and 175 MHz) NMR analyses were performed on a soluble beta-glucan preparation (GG-Zym) obtained by GG digestion with endo-beta-D-(1-->3)-glucanase and on its high- (Pool 1) and low-molecular weight (Pool 2) sub-fractions. The resonances typical of uniformly beta-D-(1-->6)- and beta-D-(1-->3)-linked linear glucans, together with additional multiplets assigned to short-chain oligoglucosides, were detected in GG-Zym. Pool 1 (46.3+/-6.4% of GG-Zym content) consisted of beta-D-(1-->6)-linked glucopyranosyl polymers, with short beta-D-(1-->3)-branched side chains of 2.20+/-0.02 units (branching degree (DB)=0.14+/-0.03). Pool 2 was a mixture of glucose and linear short-chain beta-D-(1-->3)-oligoglucosides. Further digestion of Pool 1 by beta-D-(1-->6)-glucanase yielded a mixture of glucose and short beta-D-(1-->6)-linked, either linear or beta-D-(1-->3,6) branched, oligomers. These endoglucanase digestion patterns were consistent with the presence in C. albicans cell wall glucans of beta-D-(1-->6)-linked glucopyranosyl backbones possessing beta-D-(1-->3)-linked side chains, a structure very close to that of beta-D-(1-->6)-glucan from Saccharomyces cerevisiae yeast. This finding may provide the grounds for further elucidation of the cell wall structure and a better understanding of the biological properties of C. albicans beta-glucans.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Iorio E
Department of Cell Biology and Neurosciences, Istituto Superiore di Sanità, Viale Regina Elena, 299, Roma 00161, Italy.
Torosantucci A
No affiliation info available
Bromuro C
No affiliation info available
Chiani P
No affiliation info available
Ferretti A
No affiliation info available
Giannini M
No affiliation info available
Cassone A
No affiliation info available
Podo F
No affiliation info available

MeSH

Candida albicansCell WallGlucansMagnetic Resonance Spectroscopy

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18346722

Citation

Iorio, Egidio, et al. "Candida Albicans Cell Wall Comprises a Branched beta-D-(1-->6)-glucan With beta-D-(1-->3)-side Chains." Carbohydrate Research, vol. 343, no. 6, 2008, pp. 1050-61.
Iorio E, Torosantucci A, Bromuro C, et al. Candida albicans cell wall comprises a branched beta-D-(1-->6)-glucan with beta-D-(1-->3)-side chains. Carbohydr Res. 2008;343(6):1050-61.
Iorio, E., Torosantucci, A., Bromuro, C., Chiani, P., Ferretti, A., Giannini, M., Cassone, A., & Podo, F. (2008). Candida albicans cell wall comprises a branched beta-D-(1-->6)-glucan with beta-D-(1-->3)-side chains. Carbohydrate Research, 343(6), 1050-61. https://doi.org/10.1016/j.carres.2008.02.020
Iorio E, et al. Candida Albicans Cell Wall Comprises a Branched beta-D-(1-->6)-glucan With beta-D-(1-->3)-side Chains. Carbohydr Res. 2008 May 5;343(6):1050-61. PubMed PMID: 18346722.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Candida albicans cell wall comprises a branched beta-D-(1-->6)-glucan with beta-D-(1-->3)-side chains. AU - Iorio,Egidio, AU - Torosantucci,Antonella, AU - Bromuro,Carla, AU - Chiani,Paola, AU - Ferretti,Amalia, AU - Giannini,Massimo, AU - Cassone,Antonio, AU - Podo,Franca, Y1 - 2008/02/29/ PY - 2007/10/04/received PY - 2008/02/19/revised PY - 2008/02/23/accepted PY - 2008/3/19/pubmed PY - 2008/8/20/medline PY - 2008/3/19/entrez SP - 1050 EP - 61 JF - Carbohydrate research JO - Carbohydr Res VL - 343 IS - 6 N2 - The structure of immunogenic and immunomodulatory cell wall glucans of Candida albicans is commonly interpreted in terms of a basic polysaccharide consisting of a beta-D-(1-->3)-linked glucopyranosyl backbone possessing beta-D-(1-->6)-linked side chains of varying distribution and length. This proposed molecular architecture has been re-evaluated by the present study on the products of selective enzymolysis of insoluble C. albicans glucan particles (GG). High resolution 1H (400 and 700 MHz) and 13C (100 and 175 MHz) NMR analyses were performed on a soluble beta-glucan preparation (GG-Zym) obtained by GG digestion with endo-beta-D-(1-->3)-glucanase and on its high- (Pool 1) and low-molecular weight (Pool 2) sub-fractions. The resonances typical of uniformly beta-D-(1-->6)- and beta-D-(1-->3)-linked linear glucans, together with additional multiplets assigned to short-chain oligoglucosides, were detected in GG-Zym. Pool 1 (46.3+/-6.4% of GG-Zym content) consisted of beta-D-(1-->6)-linked glucopyranosyl polymers, with short beta-D-(1-->3)-branched side chains of 2.20+/-0.02 units (branching degree (DB)=0.14+/-0.03). Pool 2 was a mixture of glucose and linear short-chain beta-D-(1-->3)-oligoglucosides. Further digestion of Pool 1 by beta-D-(1-->6)-glucanase yielded a mixture of glucose and short beta-D-(1-->6)-linked, either linear or beta-D-(1-->3,6) branched, oligomers. These endoglucanase digestion patterns were consistent with the presence in C. albicans cell wall glucans of beta-D-(1-->6)-linked glucopyranosyl backbones possessing beta-D-(1-->3)-linked side chains, a structure very close to that of beta-D-(1-->6)-glucan from Saccharomyces cerevisiae yeast. This finding may provide the grounds for further elucidation of the cell wall structure and a better understanding of the biological properties of C. albicans beta-glucans. SN - 0008-6215 UR - https://www.unboundmedicine.com/medline/citation/18346722/Candida_albicans_cell_wall_comprises_a_branched_beta_D__1__>6__glucan_with_beta_D__1__>3__side_chains_ DB - PRIME DP - Unbound Medicine ER -