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A vaccine prepared from a non-pathogenic H7N7 virus isolated from natural reservoir conferred protective immunity against the challenge with lethal dose of highly pathogenic avian influenza virus in chickens.
Vaccine. 2008 Apr 16; 26(17):2127-34.V

Abstract

During 2001-2004, 41 H7 influenza viruses (2 H7N1 and 39 H7N7 strains) were isolated from fecal samples of migratory ducks that flew from Siberia in the autumn of each year to Japan and Mongolia. A phylogenetic analysis of the hemagglutinin (HA) genes of the nine representative isolates revealed that they belonged to the Eurasian lineage and the deduced amino acid sequence at the cleavage site of the HAs represented apathogenic profiles. One of the H7 isolates A/duck/Mongolia/736/02 (H7N7) was chosen from these H7 isolates for the preparation of the test vaccine. To improve the growth potential of A/duck/Mongolia/736/02 (H7N7) in chicken embryos, A/duck/Hokkaido/Vac-2/04 (H7N7) was generated by genetic reassortment between A/duck/Mongolia/736/02 (H7N7) as the donor of the PB2, PB1, PA, HA, NA, and NS genes and A/duck/Hokkaido/49/98 (H9N2) as that of NP and M genes. The test vaccine was prepared as follows; A/duck/Hokkaido/Vac-2/04 (H7N7) was propagated in chicken embryos and the virus in the allantoic fluid was inactivated and adjuvanted to form an oil-in-water emulsion. The test vaccine conferred immunity to chickens, completely protecting the manifestation of clinical signs against the challenge with lethal dose of H7 highly pathogenic avian influenza virus. These results indicate that influenza viruses isolated from natural reservoirs are useful for vaccine strains.

Authors+Show Affiliations

Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Hokkaido 060-0818, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18346824

Citation

Sakabe, Saori, et al. "A Vaccine Prepared From a Non-pathogenic H7N7 Virus Isolated From Natural Reservoir Conferred Protective Immunity Against the Challenge With Lethal Dose of Highly Pathogenic Avian Influenza Virus in Chickens." Vaccine, vol. 26, no. 17, 2008, pp. 2127-34.
Sakabe S, Sakoda Y, Haraguchi Y, et al. A vaccine prepared from a non-pathogenic H7N7 virus isolated from natural reservoir conferred protective immunity against the challenge with lethal dose of highly pathogenic avian influenza virus in chickens. Vaccine. 2008;26(17):2127-34.
Sakabe, S., Sakoda, Y., Haraguchi, Y., Isoda, N., Soda, K., Takakuwa, H., Saijo, K., Sawata, A., Kume, K., Hagiwara, J., Tuchiya, K., Lin, Z., Sakamoto, R., Imamura, T., Sasaki, T., Kokumai, N., Kawaoka, Y., & Kida, H. (2008). A vaccine prepared from a non-pathogenic H7N7 virus isolated from natural reservoir conferred protective immunity against the challenge with lethal dose of highly pathogenic avian influenza virus in chickens. Vaccine, 26(17), 2127-34. https://doi.org/10.1016/j.vaccine.2008.02.001
Sakabe S, et al. A Vaccine Prepared From a Non-pathogenic H7N7 Virus Isolated From Natural Reservoir Conferred Protective Immunity Against the Challenge With Lethal Dose of Highly Pathogenic Avian Influenza Virus in Chickens. Vaccine. 2008 Apr 16;26(17):2127-34. PubMed PMID: 18346824.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A vaccine prepared from a non-pathogenic H7N7 virus isolated from natural reservoir conferred protective immunity against the challenge with lethal dose of highly pathogenic avian influenza virus in chickens. AU - Sakabe,Saori, AU - Sakoda,Yoshihiro, AU - Haraguchi,Yoshinari, AU - Isoda,Norikazu, AU - Soda,Kosuke, AU - Takakuwa,Hiroki, AU - Saijo,Kazue, AU - Sawata,Akira, AU - Kume,Katsumi, AU - Hagiwara,Junko, AU - Tuchiya,Kotaro, AU - Lin,Zhifeng, AU - Sakamoto,Ryuichi, AU - Imamura,Takashi, AU - Sasaki,Takashi, AU - Kokumai,Norihide, AU - Kawaoka,Yoshihiro, AU - Kida,Hiroshi, Y1 - 2008/02/22/ PY - 2007/06/13/received PY - 2008/01/16/revised PY - 2008/02/02/accepted PY - 2008/3/19/pubmed PY - 2008/11/6/medline PY - 2008/3/19/entrez SP - 2127 EP - 34 JF - Vaccine JO - Vaccine VL - 26 IS - 17 N2 - During 2001-2004, 41 H7 influenza viruses (2 H7N1 and 39 H7N7 strains) were isolated from fecal samples of migratory ducks that flew from Siberia in the autumn of each year to Japan and Mongolia. A phylogenetic analysis of the hemagglutinin (HA) genes of the nine representative isolates revealed that they belonged to the Eurasian lineage and the deduced amino acid sequence at the cleavage site of the HAs represented apathogenic profiles. One of the H7 isolates A/duck/Mongolia/736/02 (H7N7) was chosen from these H7 isolates for the preparation of the test vaccine. To improve the growth potential of A/duck/Mongolia/736/02 (H7N7) in chicken embryos, A/duck/Hokkaido/Vac-2/04 (H7N7) was generated by genetic reassortment between A/duck/Mongolia/736/02 (H7N7) as the donor of the PB2, PB1, PA, HA, NA, and NS genes and A/duck/Hokkaido/49/98 (H9N2) as that of NP and M genes. The test vaccine was prepared as follows; A/duck/Hokkaido/Vac-2/04 (H7N7) was propagated in chicken embryos and the virus in the allantoic fluid was inactivated and adjuvanted to form an oil-in-water emulsion. The test vaccine conferred immunity to chickens, completely protecting the manifestation of clinical signs against the challenge with lethal dose of H7 highly pathogenic avian influenza virus. These results indicate that influenza viruses isolated from natural reservoirs are useful for vaccine strains. SN - 0264-410X UR - https://www.unboundmedicine.com/medline/citation/18346824/A_vaccine_prepared_from_a_non_pathogenic_H7N7_virus_isolated_from_natural_reservoir_conferred_protective_immunity_against_the_challenge_with_lethal_dose_of_highly_pathogenic_avian_influenza_virus_in_chickens_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0264-410X(08)00127-8 DB - PRIME DP - Unbound Medicine ER -