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The U11-48K protein contacts the 5' splice site of U12-type introns and the U11-59K protein.
Mol Cell Biol. 2008 May; 28(10):3548-60.MC

Abstract

Little is currently known about proteins that make contact with the pre-mRNA in the U12-dependent spliceosome and thereby contribute to intron recognition. Using site-specific cross-linking, we detected an interaction between the U11-48K protein and U12-type 5' splice sites (5'ss). This interaction did not require branch point recognition and was sensitive to 5'ss mutations, suggesting that 48K interacts with the 5'ss during the first steps of prespliceosome assembly in a sequence-dependent manner. RNA interference-induced knockdown of 48K in HeLa cells led to reduced cell growth and the inhibition of U12-type splicing, as well as the activation of cryptic, U2-type splice sites, suggesting that 48K plays a critical role in U12-type intron recognition. 48K knockdown also led to reduced levels of U11/U12 di-snRNP, indicating that 48K contributes to the stability and/or formation of this complex. In addition to making contact with the 5'ss, 48K interacts with the U11-59K protein, a protein at the interface of the U11/U12 di-snRNP. These studies provide important insights into the protein-mediated recognition of the U12-type 5'ss, as well as functionally important interactions within the U11/U12 di-snRNP.

Authors+Show Affiliations

Institute of Biotechnology, PL 56 Viikinkaari 9, 00014 University of Helsinki, Helsinki, Finland.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18347052

Citation

Turunen, Janne J., et al. "The U11-48K Protein Contacts the 5' Splice Site of U12-type Introns and the U11-59K Protein." Molecular and Cellular Biology, vol. 28, no. 10, 2008, pp. 3548-60.
Turunen JJ, Will CL, Grote M, et al. The U11-48K protein contacts the 5' splice site of U12-type introns and the U11-59K protein. Mol Cell Biol. 2008;28(10):3548-60.
Turunen, J. J., Will, C. L., Grote, M., Lührmann, R., & Frilander, M. J. (2008). The U11-48K protein contacts the 5' splice site of U12-type introns and the U11-59K protein. Molecular and Cellular Biology, 28(10), 3548-60. https://doi.org/10.1128/MCB.01928-07
Turunen JJ, et al. The U11-48K Protein Contacts the 5' Splice Site of U12-type Introns and the U11-59K Protein. Mol Cell Biol. 2008;28(10):3548-60. PubMed PMID: 18347052.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The U11-48K protein contacts the 5' splice site of U12-type introns and the U11-59K protein. AU - Turunen,Janne J, AU - Will,Cindy L, AU - Grote,Michael, AU - Lührmann,Reinhard, AU - Frilander,Mikko J, Y1 - 2008/03/17/ PY - 2008/3/19/pubmed PY - 2008/5/15/medline PY - 2008/3/19/entrez SP - 3548 EP - 60 JF - Molecular and cellular biology JO - Mol Cell Biol VL - 28 IS - 10 N2 - Little is currently known about proteins that make contact with the pre-mRNA in the U12-dependent spliceosome and thereby contribute to intron recognition. Using site-specific cross-linking, we detected an interaction between the U11-48K protein and U12-type 5' splice sites (5'ss). This interaction did not require branch point recognition and was sensitive to 5'ss mutations, suggesting that 48K interacts with the 5'ss during the first steps of prespliceosome assembly in a sequence-dependent manner. RNA interference-induced knockdown of 48K in HeLa cells led to reduced cell growth and the inhibition of U12-type splicing, as well as the activation of cryptic, U2-type splice sites, suggesting that 48K plays a critical role in U12-type intron recognition. 48K knockdown also led to reduced levels of U11/U12 di-snRNP, indicating that 48K contributes to the stability and/or formation of this complex. In addition to making contact with the 5'ss, 48K interacts with the U11-59K protein, a protein at the interface of the U11/U12 di-snRNP. These studies provide important insights into the protein-mediated recognition of the U12-type 5'ss, as well as functionally important interactions within the U11/U12 di-snRNP. SN - 1098-5549 UR - https://www.unboundmedicine.com/medline/citation/18347052/The_U11_48K_protein_contacts_the_5'_splice_site_of_U12_type_introns_and_the_U11_59K_protein_ L2 - http://mcb.asm.org/cgi/pmidlookup?view=long&pmid=18347052 DB - PRIME DP - Unbound Medicine ER -