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Conjugated linoleic acids produced by Lactobacillus dissociates IKK-gamma and Hsp90 complex in Helicobacter pylori-infected gastric epithelial cells.
Lab Invest. 2008 May; 88(5):541-52.LI

Abstract

Although probiotics have been reported to reduce the gastric inflammatory response to Helicobacter pylori infection, little information is available regarding the molecular mechanisms behind this reduction. This study investigates the role of conjugated linoleic acids (CLA) produced by probiotics in interactions of IkappaB kinase (IKK) and heat shock protein 90 (Hsp90) to activate the nuclear factor-kappaB (NF-kappaB) signaling pathway in human gastric epithelial cells infected with H. pylori. Conditioned medium (CM) containing Lactobacillus acidophilus-producing CLA significantly inhibited the activated NF-kappaB signals and the upregulated expression of interleukin-8 (IL-8) in MKN-45 cells infected with H. pylori. Pretreatment with CM with CLA attenuated the increased IKK activity induced by H. pylori. Transfection of siRNA for IKK-beta dramatically reduced H. pylori-induced IkappaBalpha phosphorylation, but siRNA for IKK-alpha had little effect on IkappaBalpha phosphorylation, although the siRNA for IKK-alpha significantly decreased IL-8 production. Furthermore, Hsp90 was associated with IKK-alpha and IKK-gamma in H. pylori-infected cells, and CM with CLA dissociated the complex between Hsp90 and IKK-gamma. These results suggest that CLA produced by probiotics has anti-inflammatory activity in gastric epithelial cells infected with H. pylori via dissociation of the IKK-gamma and Hsp90 complex.

Authors+Show Affiliations

Department of Microbiology, Hanyang University College of Medicine, Seoul, South Korea. jungmogg@hanyang.ac.krNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18347582

Citation

Kim, Jung M., et al. "Conjugated Linoleic Acids Produced By Lactobacillus Dissociates IKK-gamma and Hsp90 Complex in Helicobacter Pylori-infected Gastric Epithelial Cells." Laboratory Investigation; a Journal of Technical Methods and Pathology, vol. 88, no. 5, 2008, pp. 541-52.
Kim JM, Kim JS, Kim YJ, et al. Conjugated linoleic acids produced by Lactobacillus dissociates IKK-gamma and Hsp90 complex in Helicobacter pylori-infected gastric epithelial cells. Lab Invest. 2008;88(5):541-52.
Kim, J. M., Kim, J. S., Kim, Y. J., Oh, Y. K., Kim, I. Y., Chee, Y. J., Han, J. S., & Jung, H. C. (2008). Conjugated linoleic acids produced by Lactobacillus dissociates IKK-gamma and Hsp90 complex in Helicobacter pylori-infected gastric epithelial cells. Laboratory Investigation; a Journal of Technical Methods and Pathology, 88(5), 541-52. https://doi.org/10.1038/labinvest.2008.16
Kim JM, et al. Conjugated Linoleic Acids Produced By Lactobacillus Dissociates IKK-gamma and Hsp90 Complex in Helicobacter Pylori-infected Gastric Epithelial Cells. Lab Invest. 2008;88(5):541-52. PubMed PMID: 18347582.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Conjugated linoleic acids produced by Lactobacillus dissociates IKK-gamma and Hsp90 complex in Helicobacter pylori-infected gastric epithelial cells. AU - Kim,Jung M, AU - Kim,Joo S, AU - Kim,Yeong J, AU - Oh,Yu K, AU - Kim,In Y, AU - Chee,Young J, AU - Han,Joong S, AU - Jung,Hyun C, Y1 - 2008/03/17/ PY - 2008/3/19/pubmed PY - 2008/5/9/medline PY - 2008/3/19/entrez SP - 541 EP - 52 JF - Laboratory investigation; a journal of technical methods and pathology JO - Lab Invest VL - 88 IS - 5 N2 - Although probiotics have been reported to reduce the gastric inflammatory response to Helicobacter pylori infection, little information is available regarding the molecular mechanisms behind this reduction. This study investigates the role of conjugated linoleic acids (CLA) produced by probiotics in interactions of IkappaB kinase (IKK) and heat shock protein 90 (Hsp90) to activate the nuclear factor-kappaB (NF-kappaB) signaling pathway in human gastric epithelial cells infected with H. pylori. Conditioned medium (CM) containing Lactobacillus acidophilus-producing CLA significantly inhibited the activated NF-kappaB signals and the upregulated expression of interleukin-8 (IL-8) in MKN-45 cells infected with H. pylori. Pretreatment with CM with CLA attenuated the increased IKK activity induced by H. pylori. Transfection of siRNA for IKK-beta dramatically reduced H. pylori-induced IkappaBalpha phosphorylation, but siRNA for IKK-alpha had little effect on IkappaBalpha phosphorylation, although the siRNA for IKK-alpha significantly decreased IL-8 production. Furthermore, Hsp90 was associated with IKK-alpha and IKK-gamma in H. pylori-infected cells, and CM with CLA dissociated the complex between Hsp90 and IKK-gamma. These results suggest that CLA produced by probiotics has anti-inflammatory activity in gastric epithelial cells infected with H. pylori via dissociation of the IKK-gamma and Hsp90 complex. SN - 1530-0307 UR - https://www.unboundmedicine.com/medline/citation/18347582/Conjugated_linoleic_acids_produced_by_Lactobacillus_dissociates_IKK_gamma_and_Hsp90_complex_in_Helicobacter_pylori_infected_gastric_epithelial_cells_ L2 - https://doi.org/10.1038/labinvest.2008.16 DB - PRIME DP - Unbound Medicine ER -