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IgE profiles of Bermuda grass pollen sensitised patients evaluated by Phleum pratense allergens Phl P 1, 2, 4, 5, 6 , 7, 11, 12.
Allergol Int. 2008 Jun; 57(2):157-64.AI

Abstract

BACKGROUND

Despite the difference in geographical dominance of certain grasses, a high degree of allergenic similarity or cross-reactivity between Bermuda grass pollen (BGP) and timothy grass pollen (TGP) has been previously demonstrated. The aim of the present study was to ascertain the sensitisation to TGP in 411 patients known for their reactivity to BGP extracts by analysing their reactivity to crude timothy pollen extract and timothy pollen purified allergens, establishing their specific IgE-profiles.

METHODS

Using the immunoenzymatic CAP method we evaluated IgE-specific antibodies for BGP- and TGP- extracts and the timothy recombinant (r) and natural (n) allergens rPhl p 1, rPhl p 2, nPhl p 4, rPhl p 5, rPhl p 6, rPhl p 7, rPhl p 11, and rPhl p 12.

RESULTS

BGP-IgE positive patients (median = 8.0 kUA/l, 2.8-22.2 kUA/l 25th-75th percentile) simultaneously had IgE positive results for TGP (100% of subjects)(median = 48.9 kUA/l, 19.8- > 100 kUA/l 25th-75th percentile) and high prevalence of sensitization to 6/8 Phleum pratense allergens (Phl p 1, 2, 4, 5, 6, 11, markers of genuine sensitisation to TGP) other than profilin and calcium binding protein. More than 72% of BGP allergic patients were co-sensitised to rPhl p 1, rPhl p 2, nPhl p 4, rPhl p 5, rPhl p 6. A decrease of total and specific IgE with patients' age was observed.

CONCLUSIONS

Our data show that all BGP-allergic patients simultaneously exhibit higher IgE antibody levels to recombinant and natural P. pratense allergens as well as to crude TGP extract. This suggests that when choosing an immunotherapeutic regimen for BGP-sensitised patients (after establishing their IgE profile via purified TGP-allergens), subcutaneous or sublingual TGP-extract vaccines in appropriate doses, in order to influence T epitope specificity, might be beneficial. Though extremely uncommon, in cases where a patient is exclusively BGP allergen-sensitised, BGP-extract therapy is the appropriate therapeutic response.

Authors+Show Affiliations

Allergy Unit, National Health Service, Rete di Allergologia, Regione Piemonte, Ospedali di Fossano e Savigliano, Cuneo, Italy. immunoway@tin.itNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

18349590

Citation

Rossi, Renato E., et al. "IgE Profiles of Bermuda Grass Pollen Sensitised Patients Evaluated By Phleum Pratense Allergens Phl P 1, 2, 4, 5, 6 , 7, 11, 12." Allergology International : Official Journal of the Japanese Society of Allergology, vol. 57, no. 2, 2008, pp. 157-64.
Rossi RE, Monasterolo G, Prina P, et al. IgE profiles of Bermuda grass pollen sensitised patients evaluated by Phleum pratense allergens Phl P 1, 2, 4, 5, 6 , 7, 11, 12. Allergol Int. 2008;57(2):157-64.
Rossi, R. E., Monasterolo, G., Prina, P., Coco, G., Operti, D., & Rossi, L. (2008). IgE profiles of Bermuda grass pollen sensitised patients evaluated by Phleum pratense allergens Phl P 1, 2, 4, 5, 6 , 7, 11, 12. Allergology International : Official Journal of the Japanese Society of Allergology, 57(2), 157-64. https://doi.org/10.2332/allergolint.O-07-503
Rossi RE, et al. IgE Profiles of Bermuda Grass Pollen Sensitised Patients Evaluated By Phleum Pratense Allergens Phl P 1, 2, 4, 5, 6 , 7, 11, 12. Allergol Int. 2008;57(2):157-64. PubMed PMID: 18349590.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - IgE profiles of Bermuda grass pollen sensitised patients evaluated by Phleum pratense allergens Phl P 1, 2, 4, 5, 6 , 7, 11, 12. AU - Rossi,Renato E, AU - Monasterolo,Giorgio, AU - Prina,Paolo, AU - Coco,Giuseppe, AU - Operti,Daniela, AU - Rossi,Lucilla, PY - 2007/06/05/received PY - 2007/10/25/accepted PY - 2008/3/20/pubmed PY - 2008/9/16/medline PY - 2008/3/20/entrez SP - 157 EP - 64 JF - Allergology international : official journal of the Japanese Society of Allergology JO - Allergol Int VL - 57 IS - 2 N2 - BACKGROUND: Despite the difference in geographical dominance of certain grasses, a high degree of allergenic similarity or cross-reactivity between Bermuda grass pollen (BGP) and timothy grass pollen (TGP) has been previously demonstrated. The aim of the present study was to ascertain the sensitisation to TGP in 411 patients known for their reactivity to BGP extracts by analysing their reactivity to crude timothy pollen extract and timothy pollen purified allergens, establishing their specific IgE-profiles. METHODS: Using the immunoenzymatic CAP method we evaluated IgE-specific antibodies for BGP- and TGP- extracts and the timothy recombinant (r) and natural (n) allergens rPhl p 1, rPhl p 2, nPhl p 4, rPhl p 5, rPhl p 6, rPhl p 7, rPhl p 11, and rPhl p 12. RESULTS: BGP-IgE positive patients (median = 8.0 kUA/l, 2.8-22.2 kUA/l 25th-75th percentile) simultaneously had IgE positive results for TGP (100% of subjects)(median = 48.9 kUA/l, 19.8- > 100 kUA/l 25th-75th percentile) and high prevalence of sensitization to 6/8 Phleum pratense allergens (Phl p 1, 2, 4, 5, 6, 11, markers of genuine sensitisation to TGP) other than profilin and calcium binding protein. More than 72% of BGP allergic patients were co-sensitised to rPhl p 1, rPhl p 2, nPhl p 4, rPhl p 5, rPhl p 6. A decrease of total and specific IgE with patients' age was observed. CONCLUSIONS: Our data show that all BGP-allergic patients simultaneously exhibit higher IgE antibody levels to recombinant and natural P. pratense allergens as well as to crude TGP extract. This suggests that when choosing an immunotherapeutic regimen for BGP-sensitised patients (after establishing their IgE profile via purified TGP-allergens), subcutaneous or sublingual TGP-extract vaccines in appropriate doses, in order to influence T epitope specificity, might be beneficial. Though extremely uncommon, in cases where a patient is exclusively BGP allergen-sensitised, BGP-extract therapy is the appropriate therapeutic response. SN - 1323-8930 UR - https://www.unboundmedicine.com/medline/citation/18349590/IgE_profiles_of_Bermuda_grass_pollen_sensitised_patients_evaluated_by_Phleum_pratense_allergens_Phl_P_1_2_4_5_6__7_11_12_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1323-8930(15)30767-X DB - PRIME DP - Unbound Medicine ER -