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Klebsiella pneumoniae multiresistance plasmid pMET1: similarity with the Yersinia pestis plasmid pCRY and integrative conjugative elements.
PLoS One 2008; 3(3):e1800Plos

Abstract

BACKGROUND

Dissemination of antimicrobial resistance genes has become an important public health and biodefense threat. Plasmids are important contributors to the rapid acquisition of antibiotic resistance by pathogenic bacteria.

PRINCIPAL FINDINGS

The nucleotide sequence of the Klebsiella pneumoniae multiresistance plasmid pMET1 comprises 41,723 bp and includes Tn1331.2, a transposon that carries the bla(TEM-1) gene and a perfect duplication of a 3-kbp region including the aac(6')-Ib, aadA1, and bla(OXA-9) genes. The replication region of pMET1 has been identified. Replication is independent of DNA polymerase I, and the replication region is highly related to that of the cryptic Yersinia pestis 91001 plasmid pCRY. The potential partition region has the general organization known as the parFG locus. The self-transmissible pMET1 plasmid includes a type IV secretion system consisting of proteins that make up the mating pair formation complex (Mpf) and the DNA transfer (Dtr) system. The Mpf is highly related to those in the plasmid pCRY, the mobilizable high-pathogenicity island from E. coli ECOR31 (HPI(ECOR31)), which has been proposed to be an integrative conjugative element (ICE) progenitor of high-pathogenicity islands in other Enterobacteriaceae including Yersinia species, and ICE(Kp1), an ICE found in a K. pneumoniae strain causing primary liver abscess. The Dtr MobB and MobC proteins are highly related to those of pCRY, but the endonuclease is related to that of plasmid pK245 and has no significant homology with the protein of similar function in pCRY. The region upstream of mobB includes the putative oriT and shares 90% identity with the same region in the HPI(ECOR31).

CONCLUSIONS

The comparative analyses of pMET1 with pCRY, HPI(ECOR31), and ICE(Kp1)show a very active rate of genetic exchanges between Enterobacteriaceae including Yersinia species, which represents a high public health and biodefense threat due to transfer of multiple resistance genes to pathogenic Yersinia strains.

Authors+Show Affiliations

Center for Applied Biotechnology Studies, Department of Biological Science, College of Natural Science and Mathematics, California State University Fullerton, Fullerton, California, United States of America.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

18350140

Citation

Soler Bistué, Alfonso J C., et al. "Klebsiella Pneumoniae Multiresistance Plasmid pMET1: Similarity With the Yersinia Pestis Plasmid pCRY and Integrative Conjugative Elements." PloS One, vol. 3, no. 3, 2008, pp. e1800.
Soler Bistué AJ, Birshan D, Tomaras AP, et al. Klebsiella pneumoniae multiresistance plasmid pMET1: similarity with the Yersinia pestis plasmid pCRY and integrative conjugative elements. PLoS ONE. 2008;3(3):e1800.
Soler Bistué, A. J., Birshan, D., Tomaras, A. P., Dandekar, M., Tran, T., Newmark, J., ... Tolmasky, M. E. (2008). Klebsiella pneumoniae multiresistance plasmid pMET1: similarity with the Yersinia pestis plasmid pCRY and integrative conjugative elements. PloS One, 3(3), pp. e1800. doi:10.1371/journal.pone.0001800.
Soler Bistué AJ, et al. Klebsiella Pneumoniae Multiresistance Plasmid pMET1: Similarity With the Yersinia Pestis Plasmid pCRY and Integrative Conjugative Elements. PLoS ONE. 2008 Mar 19;3(3):e1800. PubMed PMID: 18350140.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Klebsiella pneumoniae multiresistance plasmid pMET1: similarity with the Yersinia pestis plasmid pCRY and integrative conjugative elements. AU - Soler Bistué,Alfonso J C, AU - Birshan,Daniel, AU - Tomaras,Andrew P, AU - Dandekar,Manisha, AU - Tran,Tung, AU - Newmark,Jason, AU - Bui,Duyen, AU - Gupta,Nisha, AU - Hernandez,Keziah, AU - Sarno,Renee, AU - Zorreguieta,Angeles, AU - Actis,Luis A, AU - Tolmasky,Marcelo E, Y1 - 2008/03/19/ PY - 2008/01/23/received PY - 2008/02/15/accepted PY - 2008/3/20/pubmed PY - 2008/8/9/medline PY - 2008/3/20/entrez SP - e1800 EP - e1800 JF - PloS one JO - PLoS ONE VL - 3 IS - 3 N2 - BACKGROUND: Dissemination of antimicrobial resistance genes has become an important public health and biodefense threat. Plasmids are important contributors to the rapid acquisition of antibiotic resistance by pathogenic bacteria. PRINCIPAL FINDINGS: The nucleotide sequence of the Klebsiella pneumoniae multiresistance plasmid pMET1 comprises 41,723 bp and includes Tn1331.2, a transposon that carries the bla(TEM-1) gene and a perfect duplication of a 3-kbp region including the aac(6')-Ib, aadA1, and bla(OXA-9) genes. The replication region of pMET1 has been identified. Replication is independent of DNA polymerase I, and the replication region is highly related to that of the cryptic Yersinia pestis 91001 plasmid pCRY. The potential partition region has the general organization known as the parFG locus. The self-transmissible pMET1 plasmid includes a type IV secretion system consisting of proteins that make up the mating pair formation complex (Mpf) and the DNA transfer (Dtr) system. The Mpf is highly related to those in the plasmid pCRY, the mobilizable high-pathogenicity island from E. coli ECOR31 (HPI(ECOR31)), which has been proposed to be an integrative conjugative element (ICE) progenitor of high-pathogenicity islands in other Enterobacteriaceae including Yersinia species, and ICE(Kp1), an ICE found in a K. pneumoniae strain causing primary liver abscess. The Dtr MobB and MobC proteins are highly related to those of pCRY, but the endonuclease is related to that of plasmid pK245 and has no significant homology with the protein of similar function in pCRY. The region upstream of mobB includes the putative oriT and shares 90% identity with the same region in the HPI(ECOR31). CONCLUSIONS: The comparative analyses of pMET1 with pCRY, HPI(ECOR31), and ICE(Kp1)show a very active rate of genetic exchanges between Enterobacteriaceae including Yersinia species, which represents a high public health and biodefense threat due to transfer of multiple resistance genes to pathogenic Yersinia strains. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/18350140/Klebsiella_pneumoniae_multiresistance_plasmid_pMET1:_similarity_with_the_Yersinia_pestis_plasmid_pCRY_and_integrative_conjugative_elements_ L2 - http://dx.plos.org/10.1371/journal.pone.0001800 DB - PRIME DP - Unbound Medicine ER -