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Application of high-performance liquid chromatography-electrospray ionization time-of-flight mass spectrometry for analysis and quality control of Radix Astragali and its preparations.
J Chromatogr A. 2009 Mar 13; 1216(11):2087-97.JC

Abstract

A high-performance liquid chromatography coupled with electrospray ionization time-of-flight mass spectrometry (HPLC-ESI-TOF-MS) method has been developed for qualitative and quantitative analysis of isoflavonoids and saponins, as well as for the quality control of Radix Astragali and its preparations. The selectivity, reproducibility and sensitivity are compared with HPLC with diode array detection (DAD) and evaporative light scattering detection (ELSD). Limits of detection and quantification fell in ranges of 9-40 and 23-103 ng/mL for 13 analytes with a injection of 10 microL samples, and all calibration curves showed good linear regression (r(2)>0.9938) within the test range. The assay was successfully utilized to analyze the 13 marker components in 20 samples of Radix Astragali products. The quantitative results demonstrated that samples from different localities and manufacturers showed different quality. Advantages, in comparison with conventional HPLC-DAD-ELSD, are that reliable identification of target compounds could be achieved by accurate mass measurements (<3 ppm) along with characteristic retention time, extracted ions chromatograms using a narrow mass window for quantification ensure that the chromatographic peaks are free from background or co-elutes interference, and the great enhancement in selectivity and sensitivity allows identification and quantification of low levels of constituents in complex Radix Astragali matrixes.

Authors+Show Affiliations

Key Laboratory of Modern Chinese Medicines (China Pharmaceutical University), Ministry of Education, Nanjing 210009, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18353340

Citation

Qi, Lian-Wen, et al. "Application of High-performance Liquid Chromatography-electrospray Ionization Time-of-flight Mass Spectrometry for Analysis and Quality Control of Radix Astragali and Its Preparations." Journal of Chromatography. A, vol. 1216, no. 11, 2009, pp. 2087-97.
Qi LW, Li P, Ren MT, et al. Application of high-performance liquid chromatography-electrospray ionization time-of-flight mass spectrometry for analysis and quality control of Radix Astragali and its preparations. J Chromatogr A. 2009;1216(11):2087-97.
Qi, L. W., Li, P., Ren, M. T., Yu, Q. T., Wen, X. D., & Wang, Y. X. (2009). Application of high-performance liquid chromatography-electrospray ionization time-of-flight mass spectrometry for analysis and quality control of Radix Astragali and its preparations. Journal of Chromatography. A, 1216(11), 2087-97. https://doi.org/10.1016/j.chroma.2008.02.095
Qi LW, et al. Application of High-performance Liquid Chromatography-electrospray Ionization Time-of-flight Mass Spectrometry for Analysis and Quality Control of Radix Astragali and Its Preparations. J Chromatogr A. 2009 Mar 13;1216(11):2087-97. PubMed PMID: 18353340.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Application of high-performance liquid chromatography-electrospray ionization time-of-flight mass spectrometry for analysis and quality control of Radix Astragali and its preparations. AU - Qi,Lian-Wen, AU - Li,Ping, AU - Ren,Mei-Ting, AU - Yu,Qing-Tao, AU - Wen,Xiao-Dong, AU - Wang,Yu-Xia, Y1 - 2008/03/06/ PY - 2007/08/05/received PY - 2008/02/28/revised PY - 2008/02/29/accepted PY - 2008/3/21/pubmed PY - 2009/7/28/medline PY - 2008/3/21/entrez SP - 2087 EP - 97 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1216 IS - 11 N2 - A high-performance liquid chromatography coupled with electrospray ionization time-of-flight mass spectrometry (HPLC-ESI-TOF-MS) method has been developed for qualitative and quantitative analysis of isoflavonoids and saponins, as well as for the quality control of Radix Astragali and its preparations. The selectivity, reproducibility and sensitivity are compared with HPLC with diode array detection (DAD) and evaporative light scattering detection (ELSD). Limits of detection and quantification fell in ranges of 9-40 and 23-103 ng/mL for 13 analytes with a injection of 10 microL samples, and all calibration curves showed good linear regression (r(2)>0.9938) within the test range. The assay was successfully utilized to analyze the 13 marker components in 20 samples of Radix Astragali products. The quantitative results demonstrated that samples from different localities and manufacturers showed different quality. Advantages, in comparison with conventional HPLC-DAD-ELSD, are that reliable identification of target compounds could be achieved by accurate mass measurements (<3 ppm) along with characteristic retention time, extracted ions chromatograms using a narrow mass window for quantification ensure that the chromatographic peaks are free from background or co-elutes interference, and the great enhancement in selectivity and sensitivity allows identification and quantification of low levels of constituents in complex Radix Astragali matrixes. SN - 1873-3778 UR - https://www.unboundmedicine.com/medline/citation/18353340/Application_of_high_performance_liquid_chromatography_electrospray_ionization_time_of_flight_mass_spectrometry_for_analysis_and_quality_control_of_Radix_Astragali_and_its_preparations_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(08)00410-X DB - PRIME DP - Unbound Medicine ER -