Tags

Type your tag names separated by a space and hit enter

Human rhinovirus infection enhances airway epithelial cell production of growth factors involved in airway remodeling.
J Allergy Clin Immunol. 2008 May; 121(5):1238-1245.e4.JA

Abstract

BACKGROUND

Childhood human rhinovirus (HRV) infections are associated with an increased risk of asthma. We reasoned that HRV infections might be important in the pathogenesis of airway remodeling, thereby providing a mechanism by which these children are at risk of asthma.

OBJECTIVE

We sought to determine whether HRV infection of airway epithelial cells regulates production of growth factors associated with airway remodeling and to determine whether vascular endothelial growth factor (VEGF) was upregulated in airways during HRV-induced natural colds.

METHODS

Cultured human airway epithelial cells were infected with HRV. Amphiregulin, activin A, and VEGF protein levels were assayed by means of ELISA, and VEGF mRNA was quantified by using real-time RT-PCR. Pharmacologic inhibitors were used to assess the role of mitogen-activated protein kinase and nuclear factor kappaB pathways. Nasal lavage samples from subjects with confirmed natural HRV infections were assayed for VEGF protein and compared with baseline levels and with control levels.

RESULTS

HRV infection upregulated amphiregulin, activin A, and VEGF protein levels compared with control media (P < .05). VEGF gene expression was maximally induced 3 hours after infection. HRV-induced generation of VEGF was regulated by p38 mitogen-activated protein kinase and extracellular signal-regulated kinase 1/2 pathways but did not depend on nuclear factor kappaB activation. In subjects with HRV infections, VEGF levels during peak cold symptoms were significantly higher than at baseline (P = .005) or in control subjects (P < .01).

CONCLUSION

HRV-16 infection upregulates amphiregulin, activin A, and VEGF in airway epithelial cells, and HRV infections in vivo upregulate airway VEGF production.

Authors+Show Affiliations

Airway Inflammation Research Group, Department of Medicine, Institute of Infection, Immunity and Inflammation, University of Calgary, Calgary, Alberta, Canada. rleigh@ucalgary.caNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18355907

Citation

Leigh, Richard, et al. "Human Rhinovirus Infection Enhances Airway Epithelial Cell Production of Growth Factors Involved in Airway Remodeling." The Journal of Allergy and Clinical Immunology, vol. 121, no. 5, 2008, pp. 1238-1245.e4.
Leigh R, Oyelusi W, Wiehler S, et al. Human rhinovirus infection enhances airway epithelial cell production of growth factors involved in airway remodeling. J Allergy Clin Immunol. 2008;121(5):1238-1245.e4.
Leigh, R., Oyelusi, W., Wiehler, S., Koetzler, R., Zaheer, R. S., Newton, R., & Proud, D. (2008). Human rhinovirus infection enhances airway epithelial cell production of growth factors involved in airway remodeling. The Journal of Allergy and Clinical Immunology, 121(5), 1238-e4. https://doi.org/10.1016/j.jaci.2008.01.067
Leigh R, et al. Human Rhinovirus Infection Enhances Airway Epithelial Cell Production of Growth Factors Involved in Airway Remodeling. J Allergy Clin Immunol. 2008;121(5):1238-1245.e4. PubMed PMID: 18355907.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Human rhinovirus infection enhances airway epithelial cell production of growth factors involved in airway remodeling. AU - Leigh,Richard, AU - Oyelusi,Wale, AU - Wiehler,Shahina, AU - Koetzler,Rommy, AU - Zaheer,Raza S, AU - Newton,Robert, AU - Proud,David, Y1 - 2008/03/19/ PY - 2007/09/08/received PY - 2008/01/28/revised PY - 2008/01/30/accepted PY - 2008/3/22/pubmed PY - 2008/7/2/medline PY - 2008/3/22/entrez SP - 1238 EP - 1245.e4 JF - The Journal of allergy and clinical immunology JO - J Allergy Clin Immunol VL - 121 IS - 5 N2 - BACKGROUND: Childhood human rhinovirus (HRV) infections are associated with an increased risk of asthma. We reasoned that HRV infections might be important in the pathogenesis of airway remodeling, thereby providing a mechanism by which these children are at risk of asthma. OBJECTIVE: We sought to determine whether HRV infection of airway epithelial cells regulates production of growth factors associated with airway remodeling and to determine whether vascular endothelial growth factor (VEGF) was upregulated in airways during HRV-induced natural colds. METHODS: Cultured human airway epithelial cells were infected with HRV. Amphiregulin, activin A, and VEGF protein levels were assayed by means of ELISA, and VEGF mRNA was quantified by using real-time RT-PCR. Pharmacologic inhibitors were used to assess the role of mitogen-activated protein kinase and nuclear factor kappaB pathways. Nasal lavage samples from subjects with confirmed natural HRV infections were assayed for VEGF protein and compared with baseline levels and with control levels. RESULTS: HRV infection upregulated amphiregulin, activin A, and VEGF protein levels compared with control media (P < .05). VEGF gene expression was maximally induced 3 hours after infection. HRV-induced generation of VEGF was regulated by p38 mitogen-activated protein kinase and extracellular signal-regulated kinase 1/2 pathways but did not depend on nuclear factor kappaB activation. In subjects with HRV infections, VEGF levels during peak cold symptoms were significantly higher than at baseline (P = .005) or in control subjects (P < .01). CONCLUSION: HRV-16 infection upregulates amphiregulin, activin A, and VEGF in airway epithelial cells, and HRV infections in vivo upregulate airway VEGF production. SN - 1097-6825 UR - https://www.unboundmedicine.com/medline/citation/18355907/Human_rhinovirus_infection_enhances_airway_epithelial_cell_production_of_growth_factors_involved_in_airway_remodeling_ DB - PRIME DP - Unbound Medicine ER -