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Genotoxicity of cadmium on root meristem cells of Allium cepa: cytogenetic and Comet assay approach.
Ecotoxicol Environ Saf. 2008 Nov; 71(3):711-6.EE

Abstract

Cytogenetic and DNA damaging effects of cadmium (Cd) were examined through chromosome aberrations (CA) and Comet assay on the root meristem cells of Allium cepa. Root meristem cells were exposed to Cd (10, 20, and 40 μM) for 24h and also left in water for 24h recovery. Exposure of Cd revealed significant (P<0.001) inhibition of mitotic index (MI), induction of CA, mitotic aberrations (MA), and micronucleus (MN) formation. Similarly, at 40 μM significant increase in the tail length (85.70 ± 9.40 μm) and tail moment (58.75 ± 5.30 μm) was observed as compared to control 4.50 ± 0.76 and 0.86 ± 0.03 μm, respectively. However, cells examined at 24h post-exposure showed concentration-dependent decline in all the endpoints. Findings of this study confirms the root meristem cells of A. cepa are suitable model for detecting both the environmentally induced CA as well as DNA damage analyzed by Comet assay.

Authors+Show Affiliations

Ecotoxicology Section, Industrial Toxicology Research Centre, Post Box No. 80, M.G. Marg, Lucknow 226001, India.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18358534

Citation

Seth, C S., et al. "Genotoxicity of Cadmium On Root Meristem Cells of Allium Cepa: Cytogenetic and Comet Assay Approach." Ecotoxicology and Environmental Safety, vol. 71, no. 3, 2008, pp. 711-6.
Seth CS, Misra V, Chauhan LK, et al. Genotoxicity of cadmium on root meristem cells of Allium cepa: cytogenetic and Comet assay approach. Ecotoxicol Environ Saf. 2008;71(3):711-6.
Seth, C. S., Misra, V., Chauhan, L. K., & Singh, R. R. (2008). Genotoxicity of cadmium on root meristem cells of Allium cepa: cytogenetic and Comet assay approach. Ecotoxicology and Environmental Safety, 71(3), 711-6. https://doi.org/10.1016/j.ecoenv.2008.02.003
Seth CS, et al. Genotoxicity of Cadmium On Root Meristem Cells of Allium Cepa: Cytogenetic and Comet Assay Approach. Ecotoxicol Environ Saf. 2008;71(3):711-6. PubMed PMID: 18358534.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genotoxicity of cadmium on root meristem cells of Allium cepa: cytogenetic and Comet assay approach. AU - Seth,C S, AU - Misra,V, AU - Chauhan,L K S, AU - Singh,R R, Y1 - 2008/03/20/ PY - 2007/11/08/received PY - 2008/02/01/revised PY - 2008/02/12/accepted PY - 2008/3/25/pubmed PY - 2011/7/16/medline PY - 2008/3/25/entrez SP - 711 EP - 6 JF - Ecotoxicology and environmental safety JO - Ecotoxicol Environ Saf VL - 71 IS - 3 N2 - Cytogenetic and DNA damaging effects of cadmium (Cd) were examined through chromosome aberrations (CA) and Comet assay on the root meristem cells of Allium cepa. Root meristem cells were exposed to Cd (10, 20, and 40 μM) for 24h and also left in water for 24h recovery. Exposure of Cd revealed significant (P<0.001) inhibition of mitotic index (MI), induction of CA, mitotic aberrations (MA), and micronucleus (MN) formation. Similarly, at 40 μM significant increase in the tail length (85.70 ± 9.40 μm) and tail moment (58.75 ± 5.30 μm) was observed as compared to control 4.50 ± 0.76 and 0.86 ± 0.03 μm, respectively. However, cells examined at 24h post-exposure showed concentration-dependent decline in all the endpoints. Findings of this study confirms the root meristem cells of A. cepa are suitable model for detecting both the environmentally induced CA as well as DNA damage analyzed by Comet assay. SN - 1090-2414 UR - https://www.unboundmedicine.com/medline/citation/18358534/Genotoxicity_of_cadmium_on_root_meristem_cells_of_Allium_cepa:_cytogenetic_and_Comet_assay_approach_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0147-6513(08)00053-5 DB - PRIME DP - Unbound Medicine ER -