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Cross-protection of hepatitis E virus genotypes 1 and 4 in rhesus macaques.
J Med Virol. 2008 May; 80(5):824-32.JM

Abstract

The purpose of this study was to determine cross-protection between HEV genotypes 1 and 4, which are prevalent in China. Fecal suspensions of genotypes 1 and 4 from patients, as well as genotype 4 from swine, were inoculated intravenously into rhesus macaques. Each inoculum contained 5 x 10(4) genome equivalents of HEV. After infection, serum and fecal samples were collected serially and the levels of alanine aminotransferase (ALT) and anti-HEV IgG and IgM in sera, and HEV RNA in fecal samples, were measured. Liver biopsies were carried out. All the infected monkeys (12/12) developed anti-HEV IgG and exhibited fecal shedding of virus. IgM was detected in 11 of 12, and ALT elevation occurred about 2-6 weeks post-inoculation in 10 of 12, infected monkeys. Hepatic histopathology was consistent with acute viral hepatitis and the ORF2 antigen of HEV was detected in the granular cytoplasm of hepatocytes by immunohistochemistry. After recovery from their initial HEV infection, the monkeys were challenged with a heterologous genotype or heterologous source of HEV and monitored for hepatitis and fecal shedding. Previous infection with HEV completely or partially protected against subsequent challenge with a heterologous virus, because 7 of 11 monkeys did not develop HEV infection or shed virus in the feces, and none of them developed hepatitis or exhibited ALT elevation or liver biopsy findings of hepatitis. In conclusion, previous HEV infection may give rise to cross-genotype and cross-host-species protection.

Authors+Show Affiliations

Department of Cell Biology, National Institute for the Control of Pharmaceutical and Biological Products, Beijing, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18360896

Citation

Huang, Weijin, et al. "Cross-protection of Hepatitis E Virus Genotypes 1 and 4 in Rhesus Macaques." Journal of Medical Virology, vol. 80, no. 5, 2008, pp. 824-32.
Huang W, Zhang H, Harrison TJ, et al. Cross-protection of hepatitis E virus genotypes 1 and 4 in rhesus macaques. J Med Virol. 2008;80(5):824-32.
Huang, W., Zhang, H., Harrison, T. J., Lang, S., Huang, G., & Wang, Y. (2008). Cross-protection of hepatitis E virus genotypes 1 and 4 in rhesus macaques. Journal of Medical Virology, 80(5), 824-32. https://doi.org/10.1002/jmv.21140
Huang W, et al. Cross-protection of Hepatitis E Virus Genotypes 1 and 4 in Rhesus Macaques. J Med Virol. 2008;80(5):824-32. PubMed PMID: 18360896.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cross-protection of hepatitis E virus genotypes 1 and 4 in rhesus macaques. AU - Huang,Weijin, AU - Zhang,Huayuan, AU - Harrison,Tim J, AU - Lang,Shuhui, AU - Huang,Guoyong, AU - Wang,Youchun, PY - 2008/3/25/pubmed PY - 2008/5/14/medline PY - 2008/3/25/entrez SP - 824 EP - 32 JF - Journal of medical virology JO - J Med Virol VL - 80 IS - 5 N2 - The purpose of this study was to determine cross-protection between HEV genotypes 1 and 4, which are prevalent in China. Fecal suspensions of genotypes 1 and 4 from patients, as well as genotype 4 from swine, were inoculated intravenously into rhesus macaques. Each inoculum contained 5 x 10(4) genome equivalents of HEV. After infection, serum and fecal samples were collected serially and the levels of alanine aminotransferase (ALT) and anti-HEV IgG and IgM in sera, and HEV RNA in fecal samples, were measured. Liver biopsies were carried out. All the infected monkeys (12/12) developed anti-HEV IgG and exhibited fecal shedding of virus. IgM was detected in 11 of 12, and ALT elevation occurred about 2-6 weeks post-inoculation in 10 of 12, infected monkeys. Hepatic histopathology was consistent with acute viral hepatitis and the ORF2 antigen of HEV was detected in the granular cytoplasm of hepatocytes by immunohistochemistry. After recovery from their initial HEV infection, the monkeys were challenged with a heterologous genotype or heterologous source of HEV and monitored for hepatitis and fecal shedding. Previous infection with HEV completely or partially protected against subsequent challenge with a heterologous virus, because 7 of 11 monkeys did not develop HEV infection or shed virus in the feces, and none of them developed hepatitis or exhibited ALT elevation or liver biopsy findings of hepatitis. In conclusion, previous HEV infection may give rise to cross-genotype and cross-host-species protection. SN - 0146-6615 UR - https://www.unboundmedicine.com/medline/citation/18360896/Cross_protection_of_hepatitis_E_virus_genotypes_1_and_4_in_rhesus_macaques_ L2 - https://doi.org/10.1002/jmv.21140 DB - PRIME DP - Unbound Medicine ER -