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Methicillin-resistant Staphylococcus saprophyticus isolates carrying staphylococcal cassette chromosome mec have emerged in urogenital tract infections.
Antimicrob Agents Chemother. 2008 Jun; 52(6):2061-8.AA

Abstract

Staphylococcus saprophyticus is a uropathogenic bacterium that causes acute uncomplicated urinary tract infections, particularly in female outpatients. We investigated the dissemination and antimicrobial susceptibilities of 101 S. saprophyticus isolates from the genitourinary tracts of patients in Japan. Eight of these isolates were mecA positive and showed beta-lactam resistance. Pulsed-field gel electrophoresis showed that only some isolates were isogenic, indicating that the mecA gene was apparently acquired independently by mecA-positive isolates through staphylococcal cassette chromosome mec (SCCmec). Type determination of SCCmec by multiplex PCR showed a nontypeable element in the eight mecA-positive isolates. Sequence analysis of the entire SCCmec element from a prototype S. saprophyticus strain revealed that it was nontypeable with the current SCCmec classification due to the novel composition of the class A mec gene complex (IS431-mecA-mecR1-mecI genes) and the ccrA1/ccrB3 gene complex. Intriguingly, the attachment sites of SCCmec are similar to those of type I SCCmec in S. aureus NCTC 10442. Furthermore, the genes around the mec gene complex are similar to those of type II/III SCCmec in S. aureus, while those around the ccr gene complex are similar to those of SCC15305RM found in S. saprophyticus ATCC 15305. In comparison with known SCCmec elements, this S. saprophyticus SCCmec is a novel type.

Authors+Show Affiliations

Department of Microbiology, Graduate School of Comprehensive Human Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8575, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18362191

Citation

Higashide, Masato, et al. "Methicillin-resistant Staphylococcus Saprophyticus Isolates Carrying Staphylococcal Cassette Chromosome Mec Have Emerged in Urogenital Tract Infections." Antimicrobial Agents and Chemotherapy, vol. 52, no. 6, 2008, pp. 2061-8.
Higashide M, Kuroda M, Omura CT, et al. Methicillin-resistant Staphylococcus saprophyticus isolates carrying staphylococcal cassette chromosome mec have emerged in urogenital tract infections. Antimicrob Agents Chemother. 2008;52(6):2061-8.
Higashide, M., Kuroda, M., Omura, C. T., Kumano, M., Ohkawa, S., Ichimura, S., & Ohta, T. (2008). Methicillin-resistant Staphylococcus saprophyticus isolates carrying staphylococcal cassette chromosome mec have emerged in urogenital tract infections. Antimicrobial Agents and Chemotherapy, 52(6), 2061-8. https://doi.org/10.1128/AAC.01150-07
Higashide M, et al. Methicillin-resistant Staphylococcus Saprophyticus Isolates Carrying Staphylococcal Cassette Chromosome Mec Have Emerged in Urogenital Tract Infections. Antimicrob Agents Chemother. 2008;52(6):2061-8. PubMed PMID: 18362191.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Methicillin-resistant Staphylococcus saprophyticus isolates carrying staphylococcal cassette chromosome mec have emerged in urogenital tract infections. AU - Higashide,Masato, AU - Kuroda,Makoto, AU - Omura,Carlos Takashi Neves, AU - Kumano,Miyuki, AU - Ohkawa,Saburo, AU - Ichimura,Sadahiro, AU - Ohta,Toshiko, Y1 - 2008/03/24/ PY - 2008/3/26/pubmed PY - 2008/7/17/medline PY - 2008/3/26/entrez SP - 2061 EP - 8 JF - Antimicrobial agents and chemotherapy JO - Antimicrob Agents Chemother VL - 52 IS - 6 N2 - Staphylococcus saprophyticus is a uropathogenic bacterium that causes acute uncomplicated urinary tract infections, particularly in female outpatients. We investigated the dissemination and antimicrobial susceptibilities of 101 S. saprophyticus isolates from the genitourinary tracts of patients in Japan. Eight of these isolates were mecA positive and showed beta-lactam resistance. Pulsed-field gel electrophoresis showed that only some isolates were isogenic, indicating that the mecA gene was apparently acquired independently by mecA-positive isolates through staphylococcal cassette chromosome mec (SCCmec). Type determination of SCCmec by multiplex PCR showed a nontypeable element in the eight mecA-positive isolates. Sequence analysis of the entire SCCmec element from a prototype S. saprophyticus strain revealed that it was nontypeable with the current SCCmec classification due to the novel composition of the class A mec gene complex (IS431-mecA-mecR1-mecI genes) and the ccrA1/ccrB3 gene complex. Intriguingly, the attachment sites of SCCmec are similar to those of type I SCCmec in S. aureus NCTC 10442. Furthermore, the genes around the mec gene complex are similar to those of type II/III SCCmec in S. aureus, while those around the ccr gene complex are similar to those of SCC15305RM found in S. saprophyticus ATCC 15305. In comparison with known SCCmec elements, this S. saprophyticus SCCmec is a novel type. SN - 1098-6596 UR - https://www.unboundmedicine.com/medline/citation/18362191/Methicillin_resistant_Staphylococcus_saprophyticus_isolates_carrying_staphylococcal_cassette_chromosome_mec_have_emerged_in_urogenital_tract_infections_ L2 - http://aac.asm.org/cgi/pmidlookup?view=long&pmid=18362191 DB - PRIME DP - Unbound Medicine ER -