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In situ telomerase activity in pancreatic juice may discriminate pancreatic cancer from other pancreatic diseases.
Pancreas. 2008 Apr; 36(3):236-40.P

Abstract

OBJECTIVES

Detection of telomerase activity in pancreatic juice samples by telomeric repeat amplification protocol (TRAP) has been shown to be a useful diagnostic test for pancreatic cancer. However, some investigations suggested that the activity was not specific to pancreatic juice from pancreatic cancer. In the present study using an in situ TRAP assay, we investigated the specificity of telomerase activity in pancreatic juice for pancreatic cancer.

METHODS

Pancreatic juice were obtained endoscopically at endoscopic retrograde cholangiopancreatography from 10 patients with pancreatic cancer, 4 with intraductal papillary neoplasm, and 3 with normal pancreas. In situ telomerase activity in pancreatic juice samples was examined by in situ TRAP assay and was compared with the results of cytological examination.

RESULTS

Of 10 samples from pancreatic cancer, high telomerase activity in situ was detected in 9 and cancer cells in 7. Of 4 samples from intraductal papillary neoplasm, low telomerase activity in situ and mild atypical cells were detected in 2. Telomerase activity in situ was detected in none of 3 samples from normal pancreas.

CONCLUSIONS

Detection of in situ telomerase activity in pancreatic juice might help discriminate pancreatic cancer from other pancreatic diseases.

Authors+Show Affiliations

Departments of Gastrointestinal Oncology, Osaka Medical Center for Cancer and Cardiovascular Diseases, Osaka, Japan. uehara-hi@mc.pref.osaka.jpNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

18362835

Citation

Uehara, Hiroyuki, et al. "In Situ Telomerase Activity in Pancreatic Juice May Discriminate Pancreatic Cancer From Other Pancreatic Diseases." Pancreas, vol. 36, no. 3, 2008, pp. 236-40.
Uehara H, Nakaizumi A, Iishi H, et al. In situ telomerase activity in pancreatic juice may discriminate pancreatic cancer from other pancreatic diseases. Pancreas. 2008;36(3):236-40.
Uehara, H., Nakaizumi, A., Iishi, H., Takenaka, A., Eguchi, H., Ohigashi, H., & Ishikawa, O. (2008). In situ telomerase activity in pancreatic juice may discriminate pancreatic cancer from other pancreatic diseases. Pancreas, 36(3), 236-40. https://doi.org/10.1097/MPA.0b013e31815bc1d6
Uehara H, et al. In Situ Telomerase Activity in Pancreatic Juice May Discriminate Pancreatic Cancer From Other Pancreatic Diseases. Pancreas. 2008;36(3):236-40. PubMed PMID: 18362835.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In situ telomerase activity in pancreatic juice may discriminate pancreatic cancer from other pancreatic diseases. AU - Uehara,Hiroyuki, AU - Nakaizumi,Akihiko, AU - Iishi,Hiroyasu, AU - Takenaka,Akemi, AU - Eguchi,Hidetoshi, AU - Ohigashi,Hiroaki, AU - Ishikawa,Osamu, PY - 2008/3/26/pubmed PY - 2008/5/1/medline PY - 2008/3/26/entrez SP - 236 EP - 40 JF - Pancreas JO - Pancreas VL - 36 IS - 3 N2 - OBJECTIVES: Detection of telomerase activity in pancreatic juice samples by telomeric repeat amplification protocol (TRAP) has been shown to be a useful diagnostic test for pancreatic cancer. However, some investigations suggested that the activity was not specific to pancreatic juice from pancreatic cancer. In the present study using an in situ TRAP assay, we investigated the specificity of telomerase activity in pancreatic juice for pancreatic cancer. METHODS: Pancreatic juice were obtained endoscopically at endoscopic retrograde cholangiopancreatography from 10 patients with pancreatic cancer, 4 with intraductal papillary neoplasm, and 3 with normal pancreas. In situ telomerase activity in pancreatic juice samples was examined by in situ TRAP assay and was compared with the results of cytological examination. RESULTS: Of 10 samples from pancreatic cancer, high telomerase activity in situ was detected in 9 and cancer cells in 7. Of 4 samples from intraductal papillary neoplasm, low telomerase activity in situ and mild atypical cells were detected in 2. Telomerase activity in situ was detected in none of 3 samples from normal pancreas. CONCLUSIONS: Detection of in situ telomerase activity in pancreatic juice might help discriminate pancreatic cancer from other pancreatic diseases. SN - 1536-4828 UR - https://www.unboundmedicine.com/medline/citation/18362835/In_situ_telomerase_activity_in_pancreatic_juice_may_discriminate_pancreatic_cancer_from_other_pancreatic_diseases_ L2 - http://dx.doi.org/10.1097/MPA.0b013e31815bc1d6 DB - PRIME DP - Unbound Medicine ER -