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Immune response by nasal delivery of hepatitis B surface antigen and codelivery of a CpG ODN in alginate coated chitosan nanoparticles.
Eur J Pharm Biopharm. 2008 Jun; 69(2):405-16.EJ

Abstract

Alginate coated chitosan nanoparticles were previously developed with the aim of protecting the antigen, adsorbed on the surface of those chitosan nanoparticles, from enzymatic degradation at mucosal surfaces. In this work, this new delivery system was loaded with the recombinant hepatitis B surface antigen (HBsAg) and applied to mice by the intranasal route. Adjuvant effect of the delivery system was studied by measuring anti-HBsAg IgG in serum, anti-HBsAg sIgA in faeces extracts or nasal and vaginal secretions and interferon-gamma production in supernatants of the spleen cells. The mice were primed with 10 microg of the vaccine associated or not with nanoparticles and associated or not with 10 microg CpG oligodeoxynucleotide (ODN) followed by two sequential boosts at three week intervals. The association of HBsAg with the alginate coated chitosan nanoparticles, administered intranasally to the mice, gave rise to the humoral mucosal immune response. Humoral systemic immune response was not induced by the HBsAg loaded nanoparticles alone. The generation of Th1-biased antigen-specific systemic antibodies, however, was observed when HBsAg loaded nanoparticles were applied together with a second adjuvant, the immunopotentiator, CpG ODN. Moreover, all intranasally vaccinated groups showed higher interferon-gamma production when compared to naïve mice.

Authors+Show Affiliations

University of Coimbra, Coimbra, Portugal. olga@ci.uc.pt <olga@ci.uc.pt>No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18364251

Citation

Borges, Olga, et al. "Immune Response By Nasal Delivery of Hepatitis B Surface Antigen and Codelivery of a CpG ODN in Alginate Coated Chitosan Nanoparticles." European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Fur Pharmazeutische Verfahrenstechnik E.V, vol. 69, no. 2, 2008, pp. 405-16.
Borges O, Cordeiro-da-Silva A, Tavares J, et al. Immune response by nasal delivery of hepatitis B surface antigen and codelivery of a CpG ODN in alginate coated chitosan nanoparticles. Eur J Pharm Biopharm. 2008;69(2):405-16.
Borges, O., Cordeiro-da-Silva, A., Tavares, J., Santarém, N., de Sousa, A., Borchard, G., & Junginger, H. E. (2008). Immune response by nasal delivery of hepatitis B surface antigen and codelivery of a CpG ODN in alginate coated chitosan nanoparticles. European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Fur Pharmazeutische Verfahrenstechnik E.V, 69(2), 405-16. https://doi.org/10.1016/j.ejpb.2008.01.019
Borges O, et al. Immune Response By Nasal Delivery of Hepatitis B Surface Antigen and Codelivery of a CpG ODN in Alginate Coated Chitosan Nanoparticles. Eur J Pharm Biopharm. 2008;69(2):405-16. PubMed PMID: 18364251.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Immune response by nasal delivery of hepatitis B surface antigen and codelivery of a CpG ODN in alginate coated chitosan nanoparticles. AU - Borges,Olga, AU - Cordeiro-da-Silva,Anabela, AU - Tavares,Joana, AU - Santarém,Nuno, AU - de Sousa,Adriano, AU - Borchard,Gerrit, AU - Junginger,Hans E, Y1 - 2008/01/31/ PY - 2007/08/26/received PY - 2007/12/28/revised PY - 2008/01/15/accepted PY - 2008/3/28/pubmed PY - 2008/8/30/medline PY - 2008/3/28/entrez SP - 405 EP - 16 JF - European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V JO - Eur J Pharm Biopharm VL - 69 IS - 2 N2 - Alginate coated chitosan nanoparticles were previously developed with the aim of protecting the antigen, adsorbed on the surface of those chitosan nanoparticles, from enzymatic degradation at mucosal surfaces. In this work, this new delivery system was loaded with the recombinant hepatitis B surface antigen (HBsAg) and applied to mice by the intranasal route. Adjuvant effect of the delivery system was studied by measuring anti-HBsAg IgG in serum, anti-HBsAg sIgA in faeces extracts or nasal and vaginal secretions and interferon-gamma production in supernatants of the spleen cells. The mice were primed with 10 microg of the vaccine associated or not with nanoparticles and associated or not with 10 microg CpG oligodeoxynucleotide (ODN) followed by two sequential boosts at three week intervals. The association of HBsAg with the alginate coated chitosan nanoparticles, administered intranasally to the mice, gave rise to the humoral mucosal immune response. Humoral systemic immune response was not induced by the HBsAg loaded nanoparticles alone. The generation of Th1-biased antigen-specific systemic antibodies, however, was observed when HBsAg loaded nanoparticles were applied together with a second adjuvant, the immunopotentiator, CpG ODN. Moreover, all intranasally vaccinated groups showed higher interferon-gamma production when compared to naïve mice. SN - 0939-6411 UR - https://www.unboundmedicine.com/medline/citation/18364251/Immune_response_by_nasal_delivery_of_hepatitis_B_surface_antigen_and_codelivery_of_a_CpG_ODN_in_alginate_coated_chitosan_nanoparticles_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0939-6411(08)00023-4 DB - PRIME DP - Unbound Medicine ER -