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Characterisation of Shiga toxin-producing Escherichia coli O157 strains isolated from humans in Argentina, Australia and New Zealand.
BMC Microbiol. 2008 Mar 17; 8:46.BM

Abstract

BACKGROUND

Shiga toxin-producing Escherichia coli (STEC) is an important cause of bloody diarrhoea (BD), non-bloody diarrhoea (NBD) and the haemolytic uraemic syndrome (HUS). In Argentina and New Zealand, the most prevalent STEC serotype is O157:H7, which is responsible for the majority of HUS cases. In Australia, on the other hand, STEC O157:H7 is associated with a minority of HUS cases. The main aims of this study were to compare the phenotypic and genotypic characteristics of STEC O157 strains isolated between 1993 and 1996 from humans in Argentina, Australia and New Zealand, and to establish their clonal relatedness.

RESULTS

Seventy-three O157 STEC strains, isolated from HUS (n = 36), BD (n = 20), NBD (n = 10), or unspecified conditions (n = 7) in Argentina, Australia and New Zealand, were analysed. The strains were confirmed to be E. coli O157 by biochemical tests and serotyping. A multiplex polymerase chain reaction (PCR) was used to amplify the stx1, stx2 and rfbO157 genes and a genotyping method based on PCR-RFLP was used to determine stx1 and stx2 variants. This analysis revealed that the most frequent stx genotypes were stx2/stx2c (vh-a) (91%) in Argentina, stx2 (89%) in New Zealand, and stx1/stx2 (30%) in Australia. No stx1-postive strains were identified in Argentina or New Zealand. All strains harboured the eae gene and 72 strains produced enterohaemolysin (EHEC-Hly). The clonal relatedness of strains was investigated by phage typing and pulsed-field gel electrophoresis (PFGE). The most frequent phage types (PT) identified in Argentinian, Australian, and New Zealand strains were PT49 (n = 12), PT14 (n = 9), and PT2 (n = 15), respectively. Forty-six different patterns were obtained by XbaI-PFGE; 37 strains were grouped in 10 clusters and 36 strains showed unique patterns. Most clusters could be further subdivided by BlnI-PFGE.

CONCLUSION

STEC O157 strains isolated in Argentina, Australia, and New Zealand differed from each other in terms of stx-genotype and phage type. Additionally, no common PFGE patterns were found in strains isolated in the three countries. International collaborative studies of the type reported here are needed to detect and monitor potentially hypervirulent STEC clones.

Authors+Show Affiliations

Servicio Fisiopatogenia, Instituto Nacional de Enfermedades Infecciosas - ANLIS Dr. Carlos G. Malbrán. Av. Vélez Sarsfield 563 (1281) Ciudad Autónoma de Buenos Aires, Argentina.galeotta@anlis.gov.arNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18366637

Citation

Leotta, Gerardo A., et al. "Characterisation of Shiga Toxin-producing Escherichia Coli O157 Strains Isolated From Humans in Argentina, Australia and New Zealand." BMC Microbiology, vol. 8, 2008, p. 46.
Leotta GA, Miliwebsky ES, Chinen I, et al. Characterisation of Shiga toxin-producing Escherichia coli O157 strains isolated from humans in Argentina, Australia and New Zealand. BMC Microbiol. 2008;8:46.
Leotta, G. A., Miliwebsky, E. S., Chinen, I., Espinosa, E. M., Azzopardi, K., Tennant, S. M., Robins-Browne, R. M., & Rivas, M. (2008). Characterisation of Shiga toxin-producing Escherichia coli O157 strains isolated from humans in Argentina, Australia and New Zealand. BMC Microbiology, 8, 46. https://doi.org/10.1186/1471-2180-8-46
Leotta GA, et al. Characterisation of Shiga Toxin-producing Escherichia Coli O157 Strains Isolated From Humans in Argentina, Australia and New Zealand. BMC Microbiol. 2008 Mar 17;8:46. PubMed PMID: 18366637.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterisation of Shiga toxin-producing Escherichia coli O157 strains isolated from humans in Argentina, Australia and New Zealand. AU - Leotta,Gerardo A, AU - Miliwebsky,Elizabeth S, AU - Chinen,Isabel, AU - Espinosa,Estela M, AU - Azzopardi,Kristy, AU - Tennant,Sharon M, AU - Robins-Browne,Roy M, AU - Rivas,Marta, Y1 - 2008/03/17/ PY - 2007/06/25/received PY - 2008/03/17/accepted PY - 2008/3/28/pubmed PY - 2008/5/23/medline PY - 2008/3/28/entrez SP - 46 EP - 46 JF - BMC microbiology JO - BMC Microbiol. VL - 8 N2 - BACKGROUND: Shiga toxin-producing Escherichia coli (STEC) is an important cause of bloody diarrhoea (BD), non-bloody diarrhoea (NBD) and the haemolytic uraemic syndrome (HUS). In Argentina and New Zealand, the most prevalent STEC serotype is O157:H7, which is responsible for the majority of HUS cases. In Australia, on the other hand, STEC O157:H7 is associated with a minority of HUS cases. The main aims of this study were to compare the phenotypic and genotypic characteristics of STEC O157 strains isolated between 1993 and 1996 from humans in Argentina, Australia and New Zealand, and to establish their clonal relatedness. RESULTS: Seventy-three O157 STEC strains, isolated from HUS (n = 36), BD (n = 20), NBD (n = 10), or unspecified conditions (n = 7) in Argentina, Australia and New Zealand, were analysed. The strains were confirmed to be E. coli O157 by biochemical tests and serotyping. A multiplex polymerase chain reaction (PCR) was used to amplify the stx1, stx2 and rfbO157 genes and a genotyping method based on PCR-RFLP was used to determine stx1 and stx2 variants. This analysis revealed that the most frequent stx genotypes were stx2/stx2c (vh-a) (91%) in Argentina, stx2 (89%) in New Zealand, and stx1/stx2 (30%) in Australia. No stx1-postive strains were identified in Argentina or New Zealand. All strains harboured the eae gene and 72 strains produced enterohaemolysin (EHEC-Hly). The clonal relatedness of strains was investigated by phage typing and pulsed-field gel electrophoresis (PFGE). The most frequent phage types (PT) identified in Argentinian, Australian, and New Zealand strains were PT49 (n = 12), PT14 (n = 9), and PT2 (n = 15), respectively. Forty-six different patterns were obtained by XbaI-PFGE; 37 strains were grouped in 10 clusters and 36 strains showed unique patterns. Most clusters could be further subdivided by BlnI-PFGE. CONCLUSION: STEC O157 strains isolated in Argentina, Australia, and New Zealand differed from each other in terms of stx-genotype and phage type. Additionally, no common PFGE patterns were found in strains isolated in the three countries. International collaborative studies of the type reported here are needed to detect and monitor potentially hypervirulent STEC clones. SN - 1471-2180 UR - https://www.unboundmedicine.com/medline/citation/18366637/Characterisation_of_Shiga_toxin_producing_Escherichia_coli_O157_strains_isolated_from_humans_in_Argentina_Australia_and_New_Zealand_ L2 - https://bmcmicrobiol.biomedcentral.com/articles/10.1186/1471-2180-8-46 DB - PRIME DP - Unbound Medicine ER -