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Simultaneous quantification of major phytohormones and related compounds in crude plant extracts by liquid chromatography-electrospray tandem mass spectrometry.
Phytochemistry. 2008 May; 69(8):1773-81.P

Abstract

A rapid and sensitive method was developed for simultaneous quantification of multiple classes of phytohormones and some related metabolites in crude plant extracts without purification or derivatization. High-performance liquid chromatography and electrospray ionization-tandem mass spectrometry with multiple reaction monitoring were used to quantify auxins, cytokinins, abscisic acid, gibberellins, jasmonates, salicylates, and a number of related metabolites in crude plant extracts. The technology was applied to analyze biotic and abiotic stress-induced changes of phytohormones in Arabidopsis tissues, starting with 50-100mg fresh tissue. Biotic and/or abiotic stresses were shown to differentially affect levels of salicylic acid, jasmonic acid, indole-3-acetic acid, and benzoic acid, in comparison to their methyl esters. Compared with previous methods, sample preparation time and amount of sample required for analysis of phytohormones are reduced, and more classes of hormones are quantitatively profiled. Structurally diverse compounds from complicated biological matrices are determined with high selectivity and sensitivity.

Authors+Show Affiliations

Department of Biology, University of Missouri, St. Louis, MO 63121, United States.No affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

18367217

Citation

Pan, Xiangqing, et al. "Simultaneous Quantification of Major Phytohormones and Related Compounds in Crude Plant Extracts By Liquid Chromatography-electrospray Tandem Mass Spectrometry." Phytochemistry, vol. 69, no. 8, 2008, pp. 1773-81.
Pan X, Welti R, Wang X. Simultaneous quantification of major phytohormones and related compounds in crude plant extracts by liquid chromatography-electrospray tandem mass spectrometry. Phytochemistry. 2008;69(8):1773-81.
Pan, X., Welti, R., & Wang, X. (2008). Simultaneous quantification of major phytohormones and related compounds in crude plant extracts by liquid chromatography-electrospray tandem mass spectrometry. Phytochemistry, 69(8), 1773-81. https://doi.org/10.1016/j.phytochem.2008.02.008
Pan X, Welti R, Wang X. Simultaneous Quantification of Major Phytohormones and Related Compounds in Crude Plant Extracts By Liquid Chromatography-electrospray Tandem Mass Spectrometry. Phytochemistry. 2008;69(8):1773-81. PubMed PMID: 18367217.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Simultaneous quantification of major phytohormones and related compounds in crude plant extracts by liquid chromatography-electrospray tandem mass spectrometry. AU - Pan,Xiangqing, AU - Welti,Ruth, AU - Wang,Xuemin, Y1 - 2008/03/25/ PY - 2007/04/15/received PY - 2007/10/29/revised PY - 2008/02/07/accepted PY - 2008/3/28/pubmed PY - 2008/9/10/medline PY - 2008/3/28/entrez SP - 1773 EP - 81 JF - Phytochemistry JO - Phytochemistry VL - 69 IS - 8 N2 - A rapid and sensitive method was developed for simultaneous quantification of multiple classes of phytohormones and some related metabolites in crude plant extracts without purification or derivatization. High-performance liquid chromatography and electrospray ionization-tandem mass spectrometry with multiple reaction monitoring were used to quantify auxins, cytokinins, abscisic acid, gibberellins, jasmonates, salicylates, and a number of related metabolites in crude plant extracts. The technology was applied to analyze biotic and abiotic stress-induced changes of phytohormones in Arabidopsis tissues, starting with 50-100mg fresh tissue. Biotic and/or abiotic stresses were shown to differentially affect levels of salicylic acid, jasmonic acid, indole-3-acetic acid, and benzoic acid, in comparison to their methyl esters. Compared with previous methods, sample preparation time and amount of sample required for analysis of phytohormones are reduced, and more classes of hormones are quantitatively profiled. Structurally diverse compounds from complicated biological matrices are determined with high selectivity and sensitivity. SN - 0031-9422 UR - https://www.unboundmedicine.com/medline/citation/18367217/Simultaneous_quantification_of_major_phytohormones_and_related_compounds_in_crude_plant_extracts_by_liquid_chromatography_electrospray_tandem_mass_spectrometry_ DB - PRIME DP - Unbound Medicine ER -