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Dynamic response of the expression of hxt1, hxt5 and hxt7 transport proteins in Saccharomyces cerevisiae to perturbations in the extracellular glucose concentration.
J Biotechnol. 2008 Apr 30; 134(3-4):203-10.JB

Abstract

Glucose transport in Saccharomyces cerevisiae relies on a multi-factorial uptake system. The modulation of its efficiency depends on the differential expression of various sets of hexose transport-related proteins whose glucose affinity differs considerably. The expression of three different glucose transport proteins (HXT1, HXT5 and HXT6/7 with low-, intermediate- and high-affinity, respectively) was monitored as a result of modified extracellular glucose concentrations. Cultivation at glucose-limited (continuous) conditions was instantly replaced by a batch (and thus, non-limited) mode. Further, to mimic concentration gradients in large-scale production bioreactors, multiple and rapid transient glucose pulses were applied to chemostat cultivation. Antibodies against the HXT-proteins were used to monitor the proteins' expression levels prior to and after perturbing the external glucose concentrations. HXT5 and HXT6/7 were either expressed during the starvation-like steady-state phases in the chemostat cultivations, whereas HXT1 could not be detected at all. HXT1, however, is subsequently expressed during the excess of glucose in the batch mode, while the HXT5 and HXT6/7 transporters were at least found to decline. These findings coincide well with the transporters' affinity profiles. As a result of repeated and rapid transient glucose pulses during continuous fermentation, especially HXT6/7 pointed out to alter the protein expression pattern.

Authors+Show Affiliations

Institute of Biochemical Engineering, University of Stuttgart, Allmandring 31, D-70569 Stuttgart, Germany.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18367282

Citation

Buziol, Stefan, et al. "Dynamic Response of the Expression of Hxt1, Hxt5 and Hxt7 Transport Proteins in Saccharomyces Cerevisiae to Perturbations in the Extracellular Glucose Concentration." Journal of Biotechnology, vol. 134, no. 3-4, 2008, pp. 203-10.
Buziol S, Warth L, Magario I, et al. Dynamic response of the expression of hxt1, hxt5 and hxt7 transport proteins in Saccharomyces cerevisiae to perturbations in the extracellular glucose concentration. J Biotechnol. 2008;134(3-4):203-10.
Buziol, S., Warth, L., Magario, I., Freund, A., Siemann-Herzberg, M., & Reuss, M. (2008). Dynamic response of the expression of hxt1, hxt5 and hxt7 transport proteins in Saccharomyces cerevisiae to perturbations in the extracellular glucose concentration. Journal of Biotechnology, 134(3-4), 203-10. https://doi.org/10.1016/j.jbiotec.2008.02.002
Buziol S, et al. Dynamic Response of the Expression of Hxt1, Hxt5 and Hxt7 Transport Proteins in Saccharomyces Cerevisiae to Perturbations in the Extracellular Glucose Concentration. J Biotechnol. 2008 Apr 30;134(3-4):203-10. PubMed PMID: 18367282.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Dynamic response of the expression of hxt1, hxt5 and hxt7 transport proteins in Saccharomyces cerevisiae to perturbations in the extracellular glucose concentration. AU - Buziol,Stefan, AU - Warth,Lydia, AU - Magario,Ivana, AU - Freund,Andreas, AU - Siemann-Herzberg,Martin, AU - Reuss,Matthias, Y1 - 2008/02/13/ PY - 2007/10/15/received PY - 2008/01/09/revised PY - 2008/02/04/accepted PY - 2008/3/28/pubmed PY - 2008/8/13/medline PY - 2008/3/28/entrez SP - 203 EP - 10 JF - Journal of biotechnology JO - J Biotechnol VL - 134 IS - 3-4 N2 - Glucose transport in Saccharomyces cerevisiae relies on a multi-factorial uptake system. The modulation of its efficiency depends on the differential expression of various sets of hexose transport-related proteins whose glucose affinity differs considerably. The expression of three different glucose transport proteins (HXT1, HXT5 and HXT6/7 with low-, intermediate- and high-affinity, respectively) was monitored as a result of modified extracellular glucose concentrations. Cultivation at glucose-limited (continuous) conditions was instantly replaced by a batch (and thus, non-limited) mode. Further, to mimic concentration gradients in large-scale production bioreactors, multiple and rapid transient glucose pulses were applied to chemostat cultivation. Antibodies against the HXT-proteins were used to monitor the proteins' expression levels prior to and after perturbing the external glucose concentrations. HXT5 and HXT6/7 were either expressed during the starvation-like steady-state phases in the chemostat cultivations, whereas HXT1 could not be detected at all. HXT1, however, is subsequently expressed during the excess of glucose in the batch mode, while the HXT5 and HXT6/7 transporters were at least found to decline. These findings coincide well with the transporters' affinity profiles. As a result of repeated and rapid transient glucose pulses during continuous fermentation, especially HXT6/7 pointed out to alter the protein expression pattern. SN - 0168-1656 UR - https://www.unboundmedicine.com/medline/citation/18367282/Dynamic_response_of_the_expression_of_hxt1_hxt5_and_hxt7_transport_proteins_in_Saccharomyces_cerevisiae_to_perturbations_in_the_extracellular_glucose_concentration_ DB - PRIME DP - Unbound Medicine ER -