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Clinical application of real-time polymerase chain reaction for diagnosis of herpetic diseases of the anterior segment of the eye.
Jpn J Ophthalmol 2008 Jan-Feb; 52(1):24-31JJ

Abstract

PURPOSE

To assess the value of quantification of herpes simplex virus (HSV) DNA for the differential diagnosis of herpetic diseases of the anterior segment of the eye.

METHODS

One hundred forty-four samples from 90 patients with ocular inflammatory diseases were examined for HSV DNA by real-time polymerase chain reaction (PCR) with primers set for the consensus sequence of HSV-1/2 DNA polymerase. The samples included corneal epithelial scrapings, tear fluid (200microl of eye wash), and aqueous humor.

RESULTS

In cases of typical herpetic epithelial keratitis, a large number of copies of HSV DNA were detected (mean, 1.0 x 10(7) copies in epithelial scrapings and 3.5 x 10(5) copies in tear fluid). In atypical epithelial keratitis cases, a smaller number of HSV DNA copies were detected. In stromal keratitis cases, the number of copies of HSV DNA in the tear fluid (mean: 4.7 x 10(2) copies) was significantly smaller than in cases of epithelial keratitis. In the aqueous humor, the number of copies was small in endotheliitis cases (mean, 2.9 x 10(2) copies/microl), but the range was great, from (1.2-4.8) x 10(5)/microl in herpetic iridocyclitis. Seventeen percent of cases in which HSV was not suspected to be involved showed a small number of copies of HSV DNA, indicating the unexpected involvement of HSV in these cases.

CONCLUSIONS

Real-time PCR is an informative method of diagnosing herpetic eye diseases and evaluating the possible involvement of HSV in other inflammatory ocular diseases.

Authors+Show Affiliations

Division of Ophthalmology and Visual Science, Faculty of Medicine, Tottori University, Tottori, Japan. kakimaruaki@yahoo.co.jpNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18369696

Citation

Kakimaru-Hasegawa, Akiko, et al. "Clinical Application of Real-time Polymerase Chain Reaction for Diagnosis of Herpetic Diseases of the Anterior Segment of the Eye." Japanese Journal of Ophthalmology, vol. 52, no. 1, 2008, pp. 24-31.
Kakimaru-Hasegawa A, Kuo CH, Komatsu N, et al. Clinical application of real-time polymerase chain reaction for diagnosis of herpetic diseases of the anterior segment of the eye. Jpn J Ophthalmol. 2008;52(1):24-31.
Kakimaru-Hasegawa, A., Kuo, C. H., Komatsu, N., Komatsu, K., Miyazaki, D., & Inoue, Y. (2008). Clinical application of real-time polymerase chain reaction for diagnosis of herpetic diseases of the anterior segment of the eye. Japanese Journal of Ophthalmology, 52(1), pp. 24-31. doi:10.1007/s10384-007-0485-7.
Kakimaru-Hasegawa A, et al. Clinical Application of Real-time Polymerase Chain Reaction for Diagnosis of Herpetic Diseases of the Anterior Segment of the Eye. Jpn J Ophthalmol. 2008;52(1):24-31. PubMed PMID: 18369696.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Clinical application of real-time polymerase chain reaction for diagnosis of herpetic diseases of the anterior segment of the eye. AU - Kakimaru-Hasegawa,Akiko, AU - Kuo,Chuan-Hui, AU - Komatsu,Naoki, AU - Komatsu,Keiko, AU - Miyazaki,Dai, AU - Inoue,Yoshitsugu, Y1 - 2008/03/28/ PY - 2007/01/29/received PY - 2007/07/30/accepted PY - 2008/3/29/pubmed PY - 2008/7/2/medline PY - 2008/3/29/entrez SP - 24 EP - 31 JF - Japanese journal of ophthalmology JO - Jpn. J. Ophthalmol. VL - 52 IS - 1 N2 - PURPOSE: To assess the value of quantification of herpes simplex virus (HSV) DNA for the differential diagnosis of herpetic diseases of the anterior segment of the eye. METHODS: One hundred forty-four samples from 90 patients with ocular inflammatory diseases were examined for HSV DNA by real-time polymerase chain reaction (PCR) with primers set for the consensus sequence of HSV-1/2 DNA polymerase. The samples included corneal epithelial scrapings, tear fluid (200microl of eye wash), and aqueous humor. RESULTS: In cases of typical herpetic epithelial keratitis, a large number of copies of HSV DNA were detected (mean, 1.0 x 10(7) copies in epithelial scrapings and 3.5 x 10(5) copies in tear fluid). In atypical epithelial keratitis cases, a smaller number of HSV DNA copies were detected. In stromal keratitis cases, the number of copies of HSV DNA in the tear fluid (mean: 4.7 x 10(2) copies) was significantly smaller than in cases of epithelial keratitis. In the aqueous humor, the number of copies was small in endotheliitis cases (mean, 2.9 x 10(2) copies/microl), but the range was great, from (1.2-4.8) x 10(5)/microl in herpetic iridocyclitis. Seventeen percent of cases in which HSV was not suspected to be involved showed a small number of copies of HSV DNA, indicating the unexpected involvement of HSV in these cases. CONCLUSIONS: Real-time PCR is an informative method of diagnosing herpetic eye diseases and evaluating the possible involvement of HSV in other inflammatory ocular diseases. SN - 0021-5155 UR - https://www.unboundmedicine.com/medline/citation/18369696/Clinical_application_of_real_time_polymerase_chain_reaction_for_diagnosis_of_herpetic_diseases_of_the_anterior_segment_of_the_eye_ L2 - https://dx.doi.org/10.1007/s10384-007-0485-7 DB - PRIME DP - Unbound Medicine ER -