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Direct electrochemistry and electrocatalysis of cytochrome c immobilized on gold nanoparticles-chitosan-carbon nanotubes-modified electrode.
Talanta. 2007 Nov 30; 74(2):206-11.T

Abstract

A robust and effective nanohybrid film based on gold nanoparticles (GNPs)/chitosan (Chit)/multi-walled carbon nanotubes (MWNTs) was prepared by a layer-by-layer self-assembly technique. Cytochrome c (Cyt c) was successfully immobilized on the nanohybrid film modified glassy carbon (GC) electrode by cyclic voltammetry. The direct electron transfer between Cyt c and the modified electrode was investigated in detail. Cyt c shows a couple of quasi-reversible and well-defined cyclic voltammetry peaks with a formal potential (E(0')) of -0.16 V (versus Ag/AgCl) in pH 7.0 phosphate buffer solution (PBS). The Cyt c/GNPs/Chit/MWNTs modified GC electrode gives an improved electrocatalytic activity towards the reduction of hydrogen peroxide (H2O2). The sensitivity is 92.21 microA mM(-1) cm(-2) and the calculated apparent Michaelis-Menten constant (K(m)(app)) is 0.791 mM, indicating a high-catalytic activity of Cyt c. The catalysis currents increase linearly to the H2O2 concentration in a wide range of 1.5 x 10(-6) to 5.1 x 10(-4)M with a correlation coefficient 0.999. The detection limit is 9.0 x 10(-7)M (at the ratio of signal to noise, S/N=3). Moreover, the modified electrode displays rapid response (5s) to H2O2, and possesses good stability and reproducibility.

Authors+Show Affiliations

Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18371631

Citation

Xiang, Cuili, et al. "Direct Electrochemistry and Electrocatalysis of Cytochrome C Immobilized On Gold Nanoparticles-chitosan-carbon Nanotubes-modified Electrode." Talanta, vol. 74, no. 2, 2007, pp. 206-11.
Xiang C, Zou Y, Sun LX, et al. Direct electrochemistry and electrocatalysis of cytochrome c immobilized on gold nanoparticles-chitosan-carbon nanotubes-modified electrode. Talanta. 2007;74(2):206-11.
Xiang, C., Zou, Y., Sun, L. X., & Xu, F. (2007). Direct electrochemistry and electrocatalysis of cytochrome c immobilized on gold nanoparticles-chitosan-carbon nanotubes-modified electrode. Talanta, 74(2), 206-11. https://doi.org/10.1016/j.talanta.2007.05.050
Xiang C, et al. Direct Electrochemistry and Electrocatalysis of Cytochrome C Immobilized On Gold Nanoparticles-chitosan-carbon Nanotubes-modified Electrode. Talanta. 2007 Nov 30;74(2):206-11. PubMed PMID: 18371631.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Direct electrochemistry and electrocatalysis of cytochrome c immobilized on gold nanoparticles-chitosan-carbon nanotubes-modified electrode. AU - Xiang,Cuili, AU - Zou,Yongjin, AU - Sun,Li-Xian, AU - Xu,Fen, Y1 - 2007/06/07/ PY - 2007/04/20/received PY - 2007/05/24/revised PY - 2007/05/24/accepted PY - 2008/3/29/pubmed PY - 2008/4/19/medline PY - 2008/3/29/entrez SP - 206 EP - 11 JF - Talanta JO - Talanta VL - 74 IS - 2 N2 - A robust and effective nanohybrid film based on gold nanoparticles (GNPs)/chitosan (Chit)/multi-walled carbon nanotubes (MWNTs) was prepared by a layer-by-layer self-assembly technique. Cytochrome c (Cyt c) was successfully immobilized on the nanohybrid film modified glassy carbon (GC) electrode by cyclic voltammetry. The direct electron transfer between Cyt c and the modified electrode was investigated in detail. Cyt c shows a couple of quasi-reversible and well-defined cyclic voltammetry peaks with a formal potential (E(0')) of -0.16 V (versus Ag/AgCl) in pH 7.0 phosphate buffer solution (PBS). The Cyt c/GNPs/Chit/MWNTs modified GC electrode gives an improved electrocatalytic activity towards the reduction of hydrogen peroxide (H2O2). The sensitivity is 92.21 microA mM(-1) cm(-2) and the calculated apparent Michaelis-Menten constant (K(m)(app)) is 0.791 mM, indicating a high-catalytic activity of Cyt c. The catalysis currents increase linearly to the H2O2 concentration in a wide range of 1.5 x 10(-6) to 5.1 x 10(-4)M with a correlation coefficient 0.999. The detection limit is 9.0 x 10(-7)M (at the ratio of signal to noise, S/N=3). Moreover, the modified electrode displays rapid response (5s) to H2O2, and possesses good stability and reproducibility. SN - 1873-3573 UR - https://www.unboundmedicine.com/medline/citation/18371631/Direct_electrochemistry_and_electrocatalysis_of_cytochrome_c_immobilized_on_gold_nanoparticles_chitosan_carbon_nanotubes_modified_electrode_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0039-9140(07)00408-0 DB - PRIME DP - Unbound Medicine ER -