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Rapid and simple determination of adenine and guanine in DNA extract by micellar electrokinetic chromatography with indirect laser-induced fluorescence detection.
Talanta. 2008 Jan 15; 74(4):1050-5.T

Abstract

This paper describes a micellar electrokinetic chromatography with indirect laser-induced fluorescence detection method for the simultaneous determination of adenine and guanine in DNA extracts from fungus, maize and soybean. The baseline separation was achieved within 2.5min with running buffer (pH 9.3) composed of 10mM borate, 20mM SDS, 3.0x10(-7)M fluorescein sodium as background reagent, applied voltage of 27.5kV, cartridge temperature of 25.0 degrees C. Good linearity relationships (correlation coefficients>0.9917) between the second-order derivative peak heights (RFU) and concentrations of the analytes (mgml(-1)) were obtained. The detection limits in second-order derivative electrophoregrams were 1.16x10(-3)mgml(-1) for adenine and 0.29x10(-3)mgml(-1) for guanine, respectively. The RSD data of intra-day for migration times and second-order derivative peak heights were less than 0.59 and 4.09%, respectively. This developed method was applied to the analysis of the two purines in DNA extracts with recoveries in the range of 85.3-110.2%. In this work, although the detection sensitivity was lower than that of direct LIF, yet the method has the advantages of rapidness and simplicity. And it would also extend the application range of LIF detector.

Authors+Show Affiliations

College of Chemistry and Life Science, Zhejiang Normal University, Jinhua 321004, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

18371748

Citation

Wang, Weiping, et al. "Rapid and Simple Determination of Adenine and Guanine in DNA Extract By Micellar Electrokinetic Chromatography With Indirect Laser-induced Fluorescence Detection." Talanta, vol. 74, no. 4, 2008, pp. 1050-5.
Wang W, Zhou L, Wang S, et al. Rapid and simple determination of adenine and guanine in DNA extract by micellar electrokinetic chromatography with indirect laser-induced fluorescence detection. Talanta. 2008;74(4):1050-5.
Wang, W., Zhou, L., Wang, S., Luo, Z., & Hu, Z. (2008). Rapid and simple determination of adenine and guanine in DNA extract by micellar electrokinetic chromatography with indirect laser-induced fluorescence detection. Talanta, 74(4), 1050-5. https://doi.org/10.1016/j.talanta.2007.06.047
Wang W, et al. Rapid and Simple Determination of Adenine and Guanine in DNA Extract By Micellar Electrokinetic Chromatography With Indirect Laser-induced Fluorescence Detection. Talanta. 2008 Jan 15;74(4):1050-5. PubMed PMID: 18371748.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Rapid and simple determination of adenine and guanine in DNA extract by micellar electrokinetic chromatography with indirect laser-induced fluorescence detection. AU - Wang,Weiping, AU - Zhou,Lei, AU - Wang,Shumin, AU - Luo,Zhi, AU - Hu,Zhide, Y1 - 2007/11/05/ PY - 2007/03/19/received PY - 2007/06/18/revised PY - 2007/06/23/accepted PY - 2008/3/29/pubmed PY - 2008/8/30/medline PY - 2008/3/29/entrez SP - 1050 EP - 5 JF - Talanta JO - Talanta VL - 74 IS - 4 N2 - This paper describes a micellar electrokinetic chromatography with indirect laser-induced fluorescence detection method for the simultaneous determination of adenine and guanine in DNA extracts from fungus, maize and soybean. The baseline separation was achieved within 2.5min with running buffer (pH 9.3) composed of 10mM borate, 20mM SDS, 3.0x10(-7)M fluorescein sodium as background reagent, applied voltage of 27.5kV, cartridge temperature of 25.0 degrees C. Good linearity relationships (correlation coefficients>0.9917) between the second-order derivative peak heights (RFU) and concentrations of the analytes (mgml(-1)) were obtained. The detection limits in second-order derivative electrophoregrams were 1.16x10(-3)mgml(-1) for adenine and 0.29x10(-3)mgml(-1) for guanine, respectively. The RSD data of intra-day for migration times and second-order derivative peak heights were less than 0.59 and 4.09%, respectively. This developed method was applied to the analysis of the two purines in DNA extracts with recoveries in the range of 85.3-110.2%. In this work, although the detection sensitivity was lower than that of direct LIF, yet the method has the advantages of rapidness and simplicity. And it would also extend the application range of LIF detector. SN - 1873-3573 UR - https://www.unboundmedicine.com/medline/citation/18371748/Rapid_and_simple_determination_of_adenine_and_guanine_in_DNA_extract_by_micellar_electrokinetic_chromatography_with_indirect_laser_induced_fluorescence_detection_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0039-9140(07)00465-1 DB - PRIME DP - Unbound Medicine ER -