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Direct electron transfer and electrocatalysis of hemoglobin in layer-by-layer films assembled with Al-MSU-S particles.
Talanta. 2008 Feb 15; 74(5):1692-8.T

Abstract

In this paper, layer-by-layer (LBL) {MSU/Hb}(n)/PDDA films assembled by alternate adsorption of positively charged hemoglobin (Hb) and negatively charged mesoporous molecular sieves of Al-MSU-S onto a glassy carbon electrode (GCE) were reported. Al-MSU-S was synthesized by the precursor of zeolite Y and ionic liquids 1-hexadecane-3-methylimidazolium bromide (CMIMB) as a template in basic medium. It exhibited larger pore diameter, pore volume and surface area. Direct electrochemical and electrocatalytic properties of Hb in these layer-by-layer films were investigated. A pair of well-defined nearly reversible cyclic voltammetric peaks was observed and the formal potential of the heme Fe(III)/Fe(II) redox couple was found to be -0.295V (vs. SCE). The influences of layer's number and the pH of the external solution to the electron transfer behavior of Hb in {MSU/Hb}(n)/PDDA films were also estimated by cyclic voltammetry and a set of optimized conditions for film fabrication was inferred. The hemoglobin in{MSU/Hb}(n)/PDDA films displayed a good electrocatalytic activity to the reduction of hydrogen peroxide, which had linear current responses from 1.0 x 10(-6) to 1.86 x 10(-4)mol/L with the detection limit of 5.0 x 10(-7)mol/L (S/N=3). The apparent Michaeli-Menten constant (K(m)(app)) was 0.368 mmol/L. Thus, this methodology shows potential application of the preparation of third-generation biosensors.

Authors+Show Affiliations

Department of Chemistry, East China Normal University, Zhongshan Road (N), Shanghai 200062, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Letter
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18371838

Citation

Sun, Zhiyu, et al. "Direct Electron Transfer and Electrocatalysis of Hemoglobin in Layer-by-layer Films Assembled With Al-MSU-S Particles." Talanta, vol. 74, no. 5, 2008, pp. 1692-8.
Sun Z, Li Y, Zhou T, et al. Direct electron transfer and electrocatalysis of hemoglobin in layer-by-layer films assembled with Al-MSU-S particles. Talanta. 2008;74(5):1692-8.
Sun, Z., Li, Y., Zhou, T., Liu, Y., Shi, G., & Jin, L. (2008). Direct electron transfer and electrocatalysis of hemoglobin in layer-by-layer films assembled with Al-MSU-S particles. Talanta, 74(5), 1692-8. https://doi.org/10.1016/j.talanta.2007.10.009
Sun Z, et al. Direct Electron Transfer and Electrocatalysis of Hemoglobin in Layer-by-layer Films Assembled With Al-MSU-S Particles. Talanta. 2008 Feb 15;74(5):1692-8. PubMed PMID: 18371838.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Direct electron transfer and electrocatalysis of hemoglobin in layer-by-layer films assembled with Al-MSU-S particles. AU - Sun,Zhiyu, AU - Li,Youqin, AU - Zhou,Tianshu, AU - Liu,Ye, AU - Shi,Guoyue, AU - Jin,Litong, Y1 - 2007/10/13/ PY - 2007/06/25/received PY - 2007/09/27/revised PY - 2007/10/01/accepted PY - 2008/3/29/pubmed PY - 2008/5/1/medline PY - 2008/3/29/entrez SP - 1692 EP - 8 JF - Talanta JO - Talanta VL - 74 IS - 5 N2 - In this paper, layer-by-layer (LBL) {MSU/Hb}(n)/PDDA films assembled by alternate adsorption of positively charged hemoglobin (Hb) and negatively charged mesoporous molecular sieves of Al-MSU-S onto a glassy carbon electrode (GCE) were reported. Al-MSU-S was synthesized by the precursor of zeolite Y and ionic liquids 1-hexadecane-3-methylimidazolium bromide (CMIMB) as a template in basic medium. It exhibited larger pore diameter, pore volume and surface area. Direct electrochemical and electrocatalytic properties of Hb in these layer-by-layer films were investigated. A pair of well-defined nearly reversible cyclic voltammetric peaks was observed and the formal potential of the heme Fe(III)/Fe(II) redox couple was found to be -0.295V (vs. SCE). The influences of layer's number and the pH of the external solution to the electron transfer behavior of Hb in {MSU/Hb}(n)/PDDA films were also estimated by cyclic voltammetry and a set of optimized conditions for film fabrication was inferred. The hemoglobin in{MSU/Hb}(n)/PDDA films displayed a good electrocatalytic activity to the reduction of hydrogen peroxide, which had linear current responses from 1.0 x 10(-6) to 1.86 x 10(-4)mol/L with the detection limit of 5.0 x 10(-7)mol/L (S/N=3). The apparent Michaeli-Menten constant (K(m)(app)) was 0.368 mmol/L. Thus, this methodology shows potential application of the preparation of third-generation biosensors. SN - 1873-3573 UR - https://www.unboundmedicine.com/medline/citation/18371838/Direct_electron_transfer_and_electrocatalysis_of_hemoglobin_in_layer_by_layer_films_assembled_with_Al_MSU_S_particles_ DB - PRIME DP - Unbound Medicine ER -