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Selective solid-phase extraction of a triterpene acid from a plant extract by molecularly imprinted polymer.
Talanta. 2008 Apr 15; 75(2):344-50.T

Abstract

A molecularly imprinted polymer (MIP) has been prepared by a thermal polymerisation method using methacrylic acid as functional monomer, ethylene glycol dimethacrylate as cross-linking agent, chloroform as porogenic solvent and an oleanane triterpene compound (18-beta-glycyrrhetinic acid) as imprinted molecule (template). Equilibrium ligand binding experiments were done to assess the performance of the MIP relative to non-imprinted polymer (NIP). After optimisation of SPE protocol (CHCl3 as washing solvent and MeOH as elution solvent), successful imprinting was confirmed by comparison of the recoveries between NIP (5%) and MIP (97%) cartridges. The binding capacity of the MIP for 18-beta-glycyrrhetinic acid was determined to be 0.94 mg g(-1). Four structurally related oleanane triterpenes (18-alpha-glycyrrhetinic acid, oleanolic acid, echinocystic acid, erythrodiol) were selected to assess the MIP selectivity. Experimental data illustrated the influence of functional groups on the triterpene skeleton. The MIP was applied to the solid-phase extraction of triterpenoids from a plant extract prior HPLC analysis. However, CHCl3 was replaced by ACN during the washing step in order to suppress non-specific interactions due to polar matrix components. A selective extraction of 18-beta-glycyrrhetinic acid from hydrolyzed extract of liquorice roots was achieved with a good extraction yield (98%).

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Publisher Full Text (DOI)

Authors+Show Affiliations

Claude B
Institut de Chimie Organique et Analytique, CNRS FR 2708 UMR 6005, Université d'Orléans, 45067 Orléans, France.
Morin P
No affiliation info available
Lafosse M
No affiliation info available
Belmont AS
No affiliation info available
Haupt K
No affiliation info available

MeSH

Chromatography, High Pressure LiquidPlant ExtractsPolymersSpectrophotometry, UltravioletTriterpenes

Pub Type(s)

Journal Article

Language

eng

PubMed ID

18371889

Citation

Claude, Bérengère, et al. "Selective Solid-phase Extraction of a Triterpene Acid From a Plant Extract By Molecularly Imprinted Polymer." Talanta, vol. 75, no. 2, 2008, pp. 344-50.
Claude B, Morin P, Lafosse M, et al. Selective solid-phase extraction of a triterpene acid from a plant extract by molecularly imprinted polymer. Talanta. 2008;75(2):344-50.
Claude, B., Morin, P., Lafosse, M., Belmont, A. S., & Haupt, K. (2008). Selective solid-phase extraction of a triterpene acid from a plant extract by molecularly imprinted polymer. Talanta, 75(2), 344-50. https://doi.org/10.1016/j.talanta.2007.11.037
Claude B, et al. Selective Solid-phase Extraction of a Triterpene Acid From a Plant Extract By Molecularly Imprinted Polymer. Talanta. 2008 Apr 15;75(2):344-50. PubMed PMID: 18371889.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Selective solid-phase extraction of a triterpene acid from a plant extract by molecularly imprinted polymer. AU - Claude,Bérengère, AU - Morin,Philippe, AU - Lafosse,Michel, AU - Belmont,Anne-Sophie, AU - Haupt,Karsten, Y1 - 2007/11/21/ PY - 2007/06/26/received PY - 2007/10/01/revised PY - 2007/11/07/accepted PY - 2008/3/29/pubmed PY - 2008/8/9/medline PY - 2008/3/29/entrez SP - 344 EP - 50 JF - Talanta JO - Talanta VL - 75 IS - 2 N2 - A molecularly imprinted polymer (MIP) has been prepared by a thermal polymerisation method using methacrylic acid as functional monomer, ethylene glycol dimethacrylate as cross-linking agent, chloroform as porogenic solvent and an oleanane triterpene compound (18-beta-glycyrrhetinic acid) as imprinted molecule (template). Equilibrium ligand binding experiments were done to assess the performance of the MIP relative to non-imprinted polymer (NIP). After optimisation of SPE protocol (CHCl3 as washing solvent and MeOH as elution solvent), successful imprinting was confirmed by comparison of the recoveries between NIP (5%) and MIP (97%) cartridges. The binding capacity of the MIP for 18-beta-glycyrrhetinic acid was determined to be 0.94 mg g(-1). Four structurally related oleanane triterpenes (18-alpha-glycyrrhetinic acid, oleanolic acid, echinocystic acid, erythrodiol) were selected to assess the MIP selectivity. Experimental data illustrated the influence of functional groups on the triterpene skeleton. The MIP was applied to the solid-phase extraction of triterpenoids from a plant extract prior HPLC analysis. However, CHCl3 was replaced by ACN during the washing step in order to suppress non-specific interactions due to polar matrix components. A selective extraction of 18-beta-glycyrrhetinic acid from hydrolyzed extract of liquorice roots was achieved with a good extraction yield (98%). SN - 1873-3573 UR - https://www.unboundmedicine.com/medline/citation/18371889/Selective_solid_phase_extraction_of_a_triterpene_acid_from_a_plant_extract_by_molecularly_imprinted_polymer_ DB - PRIME DP - Unbound Medicine ER -