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Neuronal mitochondrial amelioration by feeding acetyl-L-carnitine and lipoic acid to aged rats.
J Cell Mol Med. 2009 Feb; 13(2):320-33.JC

Abstract

Brain function declines with age and is associated with diminishing mitochondrial integrity. The neuronal mitochondrial ultrastructural changes of young (4 months) and old (21 months) F344 rats supplemented with two mitochondrial metabolites, acetyl-L-carnitine (ALCAR, 0.2%[wt/vol] in the drinking water) and R-alpha-lipoic acid (LA, 0.1%[wt/wt] in the chow), were analysed using qualitative and quantitative electron microscopy techniques. Two independent morphologists blinded to sample identity examined and scored all electron micrographs. Mitochondria were examined in each micrograph, and each structure was scored according to the degree of injury. Controls displayed an age-associated significant decrease in the number of intact mitochondria (P = 0.026) as well as an increase in mitochondria with broken cristae (P < 0.001) in the hippocampus as demonstrated by electron microscopic observations. Neuronal mitochondrial damage was associated with damage in vessel wall cells, especially vascular endothelial cells. Dietary supplementation of young and aged animals increased the proliferation of intact mitochondria and reduced the density of mitochondria associated with vacuoles and lipofuscin. Feeding old rats ALCAR and LA significantly reduced the number of severely damaged mitochondria (P = 0.02) and increased the number of intact mitochondria (P < 0.001) in the hippocampus. These results suggest that feeding ALCAR with LA may ameliorate age-associated mitochondrial ultrastructural decay and are consistent with previous studies showing improved brain function.

Authors+Show Affiliations

Department of Biology, University of Texas at San Antonio, 78249, USA. gjumrakch.aliev@utsa.eduNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18373733

Citation

Aliev, Gjumrakch, et al. "Neuronal Mitochondrial Amelioration By Feeding acetyl-L-carnitine and Lipoic Acid to Aged Rats." Journal of Cellular and Molecular Medicine, vol. 13, no. 2, 2009, pp. 320-33.
Aliev G, Liu J, Shenk JC, et al. Neuronal mitochondrial amelioration by feeding acetyl-L-carnitine and lipoic acid to aged rats. J Cell Mol Med. 2009;13(2):320-33.
Aliev, G., Liu, J., Shenk, J. C., Fischbach, K., Pacheco, G. J., Chen, S. G., Obrenovich, M. E., Ward, W. F., Richardson, A. G., Smith, M. A., Gasimov, E., Perry, G., & Ames, B. N. (2009). Neuronal mitochondrial amelioration by feeding acetyl-L-carnitine and lipoic acid to aged rats. Journal of Cellular and Molecular Medicine, 13(2), 320-33. https://doi.org/10.1111/j.1582-4934.2008.00324.x
Aliev G, et al. Neuronal Mitochondrial Amelioration By Feeding acetyl-L-carnitine and Lipoic Acid to Aged Rats. J Cell Mol Med. 2009;13(2):320-33. PubMed PMID: 18373733.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Neuronal mitochondrial amelioration by feeding acetyl-L-carnitine and lipoic acid to aged rats. AU - Aliev,Gjumrakch, AU - Liu,Jiankang, AU - Shenk,Justin C, AU - Fischbach,Kathryn, AU - Pacheco,Gerardo J, AU - Chen,Shu G, AU - Obrenovich,Mark E, AU - Ward,Walter F, AU - Richardson,Arlan G, AU - Smith,Mark A, AU - Gasimov,Eldar, AU - Perry,George, AU - Ames,Bruce N, Y1 - 2008/03/28/ PY - 2008/4/1/pubmed PY - 2009/5/12/medline PY - 2008/4/1/entrez SP - 320 EP - 33 JF - Journal of cellular and molecular medicine JO - J Cell Mol Med VL - 13 IS - 2 N2 - Brain function declines with age and is associated with diminishing mitochondrial integrity. The neuronal mitochondrial ultrastructural changes of young (4 months) and old (21 months) F344 rats supplemented with two mitochondrial metabolites, acetyl-L-carnitine (ALCAR, 0.2%[wt/vol] in the drinking water) and R-alpha-lipoic acid (LA, 0.1%[wt/wt] in the chow), were analysed using qualitative and quantitative electron microscopy techniques. Two independent morphologists blinded to sample identity examined and scored all electron micrographs. Mitochondria were examined in each micrograph, and each structure was scored according to the degree of injury. Controls displayed an age-associated significant decrease in the number of intact mitochondria (P = 0.026) as well as an increase in mitochondria with broken cristae (P < 0.001) in the hippocampus as demonstrated by electron microscopic observations. Neuronal mitochondrial damage was associated with damage in vessel wall cells, especially vascular endothelial cells. Dietary supplementation of young and aged animals increased the proliferation of intact mitochondria and reduced the density of mitochondria associated with vacuoles and lipofuscin. Feeding old rats ALCAR and LA significantly reduced the number of severely damaged mitochondria (P = 0.02) and increased the number of intact mitochondria (P < 0.001) in the hippocampus. These results suggest that feeding ALCAR with LA may ameliorate age-associated mitochondrial ultrastructural decay and are consistent with previous studies showing improved brain function. SN - 1582-4934 UR - https://www.unboundmedicine.com/medline/citation/18373733/Neuronal_mitochondrial_amelioration_by_feeding_acetyl_L_carnitine_and_lipoic_acid_to_aged_rats_ L2 - https://doi.org/10.1111/j.1582-4934.2008.00324.x DB - PRIME DP - Unbound Medicine ER -