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Close coupling between transcription and exit of mRNP from the cell nucleus.
Exp Cell Res. 2008 May 01; 314(8):1708-20.EC

Abstract

Transcription is intimately coupled to co-transcriptional formation of mRNP particles and their preparation for export. In the dipteran Chironomus tentans we have now investigated whether on-going transcription is closely linked also to the ensuing transfer of the mRNPs from genes to cytoplasm. The assembly and nucleocytoplasmic transport of a specific mRNP particle, the Balbiani ring (BR) RNP granule, were visualized in larval salivary glands by electron microscopy. When transcription was inhibited with DRB or actinomycin D (AMD), the growing BR mRNPs disappeared from the genes. The two inhibitors affected the distribution of BR mRNPs in the nucleoplasm and in the nuclear pores in essentially the same way. At the nuclear pore complexes (NPCs) the basket-associated and translocating mRNPs were substantially reduced in number, the translocating RNPs being essentially absent after 90 min treatment. Remarkably, the amount of BR mRNPs in the nucleoplasm did not change. We conclude that on-going transcription is required for the mRNPs to exit from the cell nucleus. Interruption of transcription seems to primarily affect the intranuclear movement of BR mRNPs and/or prevent the binding of mRNPs to the NPCs rather than to directly interfere with translocation per se.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Kylberg K
Department of Cell and Molecular Biology, Medical Nobel Institute, Karolinska Institutet, SE-171 77 Stockholm, Sweden.
Björkroth B
No affiliation info available
Ivarsson B
No affiliation info available
Fomproix N
No affiliation info available
Daneholt B
No affiliation info available

MeSH

Active Transport, Cell NucleusAnimalsCell NucleusChironomidaeDactinomycinDichlororibofuranosylbenzimidazoleGenes, InsectNuclear EnvelopeNucleic Acid Synthesis InhibitorsRibonucleoproteinsSalivary GlandsTranscription, Genetic

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18374333

Citation

Kylberg, Karin, et al. "Close Coupling Between Transcription and Exit of mRNP From the Cell Nucleus." Experimental Cell Research, vol. 314, no. 8, 2008, pp. 1708-20.
Kylberg K, Björkroth B, Ivarsson B, et al. Close coupling between transcription and exit of mRNP from the cell nucleus. Exp Cell Res. 2008;314(8):1708-20.
Kylberg, K., Björkroth, B., Ivarsson, B., Fomproix, N., & Daneholt, B. (2008). Close coupling between transcription and exit of mRNP from the cell nucleus. Experimental Cell Research, 314(8), 1708-20. https://doi.org/10.1016/j.yexcr.2008.02.003
Kylberg K, et al. Close Coupling Between Transcription and Exit of mRNP From the Cell Nucleus. Exp Cell Res. 2008 May 1;314(8):1708-20. PubMed PMID: 18374333.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Close coupling between transcription and exit of mRNP from the cell nucleus. AU - Kylberg,Karin, AU - Björkroth,Birgitta, AU - Ivarsson,Birgitta, AU - Fomproix,Nathalie, AU - Daneholt,Bertil, Y1 - 2008/03/10/ PY - 2007/09/03/received PY - 2008/02/05/revised PY - 2008/02/08/accepted PY - 2008/4/1/pubmed PY - 2008/6/20/medline PY - 2008/4/1/entrez SP - 1708 EP - 20 JF - Experimental cell research JO - Exp Cell Res VL - 314 IS - 8 N2 - Transcription is intimately coupled to co-transcriptional formation of mRNP particles and their preparation for export. In the dipteran Chironomus tentans we have now investigated whether on-going transcription is closely linked also to the ensuing transfer of the mRNPs from genes to cytoplasm. The assembly and nucleocytoplasmic transport of a specific mRNP particle, the Balbiani ring (BR) RNP granule, were visualized in larval salivary glands by electron microscopy. When transcription was inhibited with DRB or actinomycin D (AMD), the growing BR mRNPs disappeared from the genes. The two inhibitors affected the distribution of BR mRNPs in the nucleoplasm and in the nuclear pores in essentially the same way. At the nuclear pore complexes (NPCs) the basket-associated and translocating mRNPs were substantially reduced in number, the translocating RNPs being essentially absent after 90 min treatment. Remarkably, the amount of BR mRNPs in the nucleoplasm did not change. We conclude that on-going transcription is required for the mRNPs to exit from the cell nucleus. Interruption of transcription seems to primarily affect the intranuclear movement of BR mRNPs and/or prevent the binding of mRNPs to the NPCs rather than to directly interfere with translocation per se. SN - 0014-4827 UR - https://www.unboundmedicine.com/medline/citation/18374333/Close_coupling_between_transcription_and_exit_of_mRNP_from_the_cell_nucleus_ DB - PRIME DP - Unbound Medicine ER -