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Quantitative analysis of 3-alkyl-2-methoxypyrazines in juice and wine using stable isotope labelled internal standard assay.
J Chromatogr A. 2008 May 09; 1190(1-2):294-301.JC

Abstract

A solid phase microextraction (HS-SPME)-GC-MS methodology was established for the analysis of 3-alkyl-2-methoxypyrazines (MPs) in wine using a stable isotope dilution assay. The compounds analysed were 3-isobutyl-2-methoxypyrazine (IBMP), 3-sec-butyl-2-methoxypyrazine (SBMP), and 3-isopropyl-2-methoxypyrazine (IPMP) using their respective deuterated analogues ([2H3]-IBMP, [2H3]-SBMP, [2H3]-IPMP) as internal standards, synthesised during this work. A divinylbenzene/carboxene/polydimethylsiloxane (DVB/CAR/PDMS) fibre was selected for isolation of MPs and the effects of matrix parameters such as pH and ethanol concentration were examined in the development of the method. Best results were obtained at a pH of approximately 6 and with a wine dilution factor of 1:2.5, resulting in an ethanol concentration of approximately 5% (v/v). Relative standard deviations (RSDs) of replicate samples were 5.6-7% for all MPs at 5 ng L(-1) and <5% for 15 and 30 ng L(-1) samples. The limit of detection was <0.5 ng L(-1) in juice and 1-2 ng L(-1) in wine. The recovery efficiencies for spiked wine samples were between 99 and 102% for all three MPs. Using this method, we investigated the impact of the Multicoloured Asian Lady Beetle (MALB) on MPs in wine. In red wine fermented with live MALB, IPMP is the most prevalent MP detected, although SBMP concentrations are also increased and IBMP is unchanged from background levels. MALB that have been dead for 1 day before addition to juice can still contribute to elevated SBMP concentrations in wine, but not if they have been dead for 3 days or longer. Clarifying juice prior to fermentation leads to substantially lower IPMP concentration in the subsequent wine when compared with unclarified juice.

Authors+Show Affiliations

Department of Food Science & Technology, Agricultural University of Athens, Athens, Greece. ykotseridis@aua.gr <ykotseridis@aua.gr>No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18377916

Citation

Kotseridis, Y S., et al. "Quantitative Analysis of 3-alkyl-2-methoxypyrazines in Juice and Wine Using Stable Isotope Labelled Internal Standard Assay." Journal of Chromatography. A, vol. 1190, no. 1-2, 2008, pp. 294-301.
Kotseridis YS, Spink M, Brindle ID, et al. Quantitative analysis of 3-alkyl-2-methoxypyrazines in juice and wine using stable isotope labelled internal standard assay. J Chromatogr A. 2008;1190(1-2):294-301.
Kotseridis, Y. S., Spink, M., Brindle, I. D., Blake, A. J., Sears, M., Chen, X., Soleas, G., Inglis, D., & Pickering, G. J. (2008). Quantitative analysis of 3-alkyl-2-methoxypyrazines in juice and wine using stable isotope labelled internal standard assay. Journal of Chromatography. A, 1190(1-2), 294-301. https://doi.org/10.1016/j.chroma.2008.02.088
Kotseridis YS, et al. Quantitative Analysis of 3-alkyl-2-methoxypyrazines in Juice and Wine Using Stable Isotope Labelled Internal Standard Assay. J Chromatogr A. 2008 May 9;1190(1-2):294-301. PubMed PMID: 18377916.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Quantitative analysis of 3-alkyl-2-methoxypyrazines in juice and wine using stable isotope labelled internal standard assay. AU - Kotseridis,Y S, AU - Spink,M, AU - Brindle,I D, AU - Blake,A J, AU - Sears,M, AU - Chen,X, AU - Soleas,G, AU - Inglis,D, AU - Pickering,G J, Y1 - 2008/03/02/ PY - 2007/12/28/received PY - 2008/02/20/revised PY - 2008/02/25/accepted PY - 2008/4/2/pubmed PY - 2008/8/14/medline PY - 2008/4/2/entrez SP - 294 EP - 301 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1190 IS - 1-2 N2 - A solid phase microextraction (HS-SPME)-GC-MS methodology was established for the analysis of 3-alkyl-2-methoxypyrazines (MPs) in wine using a stable isotope dilution assay. The compounds analysed were 3-isobutyl-2-methoxypyrazine (IBMP), 3-sec-butyl-2-methoxypyrazine (SBMP), and 3-isopropyl-2-methoxypyrazine (IPMP) using their respective deuterated analogues ([2H3]-IBMP, [2H3]-SBMP, [2H3]-IPMP) as internal standards, synthesised during this work. A divinylbenzene/carboxene/polydimethylsiloxane (DVB/CAR/PDMS) fibre was selected for isolation of MPs and the effects of matrix parameters such as pH and ethanol concentration were examined in the development of the method. Best results were obtained at a pH of approximately 6 and with a wine dilution factor of 1:2.5, resulting in an ethanol concentration of approximately 5% (v/v). Relative standard deviations (RSDs) of replicate samples were 5.6-7% for all MPs at 5 ng L(-1) and <5% for 15 and 30 ng L(-1) samples. The limit of detection was <0.5 ng L(-1) in juice and 1-2 ng L(-1) in wine. The recovery efficiencies for spiked wine samples were between 99 and 102% for all three MPs. Using this method, we investigated the impact of the Multicoloured Asian Lady Beetle (MALB) on MPs in wine. In red wine fermented with live MALB, IPMP is the most prevalent MP detected, although SBMP concentrations are also increased and IBMP is unchanged from background levels. MALB that have been dead for 1 day before addition to juice can still contribute to elevated SBMP concentrations in wine, but not if they have been dead for 3 days or longer. Clarifying juice prior to fermentation leads to substantially lower IPMP concentration in the subsequent wine when compared with unclarified juice. SN - 0021-9673 UR - https://www.unboundmedicine.com/medline/citation/18377916/Quantitative_analysis_of_3_alkyl_2_methoxypyrazines_in_juice_and_wine_using_stable_isotope_labelled_internal_standard_assay_ DB - PRIME DP - Unbound Medicine ER -