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Positive regulation of promoter activity of human 3-phosphoglycerate dehydrogenase (PHGDH) gene is mediated by transcription factors Sp1 and NF-Y.
Gene. 2008 May 15; 414(1-2):106-14.GENE

Abstract

The PHGDH gene encodes the 3-phosphoglycerate dehydrogenase that catalyzes the transition of 3-phosphoglycerate into 3-phosphohydroxy pyruvate for the phosphorylated pathway of serine biosynthesis. To understand transcriptional regulation of the human PHGDH promoter, a genomic clone containing the 5'-flanking region of the PHGDH gene was isolated from a human genomic library. The 1192-bp PHGDH promoter region was cloned by PCR using the genomic DNA isolated from the PHGDH genomic clone. Sequence analysis of the promoter region exhibited several putative transcription factor binding sites for NF-Y, Sp1, GATA-1, p53, AP2, and AP1, with no TATA-box motif at an appropriate position. Transfection of a series of deletion constructs of the promoter region into HeLa cells revealed that the core positive promoter activity resided in the -276 to +1, which contains two GC-motifs for binding Sp1 and one CCAAT-motif for NF-Y. Mutational analysis and electrophoretic mobility shift assay indicated that both the proximal GC-motif and CCAAT-motif were crucial for full induction of the promoter activity. Chromatin immunoprecipitation analysis confirmed the recruitment of Sp1 and NF-Y to the promoter region in vivo. These results demonstrated that the promoter activity of the human PHGDH gene was positively regulated by the action of transcription factors Sp1 and NF-Y.

Authors+Show Affiliations

Laboratory of Immunobiology, School of Life Sciences and Biotechnology, College of Natural Sciences, Kyungpook National University, Daegu, Republic of Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18378410

Citation

Jun, Do Youn, et al. "Positive Regulation of Promoter Activity of Human 3-phosphoglycerate Dehydrogenase (PHGDH) Gene Is Mediated By Transcription Factors Sp1 and NF-Y." Gene, vol. 414, no. 1-2, 2008, pp. 106-14.
Jun DY, Park HS, Lee JY, et al. Positive regulation of promoter activity of human 3-phosphoglycerate dehydrogenase (PHGDH) gene is mediated by transcription factors Sp1 and NF-Y. Gene. 2008;414(1-2):106-14.
Jun, D. Y., Park, H. S., Lee, J. Y., Baek, J. Y., Park, H. K., Fukui, K., & Kim, Y. H. (2008). Positive regulation of promoter activity of human 3-phosphoglycerate dehydrogenase (PHGDH) gene is mediated by transcription factors Sp1 and NF-Y. Gene, 414(1-2), 106-14. https://doi.org/10.1016/j.gene.2008.02.018
Jun DY, et al. Positive Regulation of Promoter Activity of Human 3-phosphoglycerate Dehydrogenase (PHGDH) Gene Is Mediated By Transcription Factors Sp1 and NF-Y. Gene. 2008 May 15;414(1-2):106-14. PubMed PMID: 18378410.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Positive regulation of promoter activity of human 3-phosphoglycerate dehydrogenase (PHGDH) gene is mediated by transcription factors Sp1 and NF-Y. AU - Jun,Do Youn, AU - Park,Hae Sun, AU - Lee,Ji Young, AU - Baek,Joo Youn, AU - Park,Hwan-Ki, AU - Fukui,Kiyoshi, AU - Kim,Young Ho, Y1 - 2008/03/04/ PY - 2007/11/02/received PY - 2008/02/12/revised PY - 2008/02/16/accepted PY - 2008/4/2/pubmed PY - 2008/7/2/medline PY - 2008/4/2/entrez SP - 106 EP - 14 JF - Gene JO - Gene VL - 414 IS - 1-2 N2 - The PHGDH gene encodes the 3-phosphoglycerate dehydrogenase that catalyzes the transition of 3-phosphoglycerate into 3-phosphohydroxy pyruvate for the phosphorylated pathway of serine biosynthesis. To understand transcriptional regulation of the human PHGDH promoter, a genomic clone containing the 5'-flanking region of the PHGDH gene was isolated from a human genomic library. The 1192-bp PHGDH promoter region was cloned by PCR using the genomic DNA isolated from the PHGDH genomic clone. Sequence analysis of the promoter region exhibited several putative transcription factor binding sites for NF-Y, Sp1, GATA-1, p53, AP2, and AP1, with no TATA-box motif at an appropriate position. Transfection of a series of deletion constructs of the promoter region into HeLa cells revealed that the core positive promoter activity resided in the -276 to +1, which contains two GC-motifs for binding Sp1 and one CCAAT-motif for NF-Y. Mutational analysis and electrophoretic mobility shift assay indicated that both the proximal GC-motif and CCAAT-motif were crucial for full induction of the promoter activity. Chromatin immunoprecipitation analysis confirmed the recruitment of Sp1 and NF-Y to the promoter region in vivo. These results demonstrated that the promoter activity of the human PHGDH gene was positively regulated by the action of transcription factors Sp1 and NF-Y. SN - 0378-1119 UR - https://www.unboundmedicine.com/medline/citation/18378410/Positive_regulation_of_promoter_activity_of_human_3_phosphoglycerate_dehydrogenase__PHGDH__gene_is_mediated_by_transcription_factors_Sp1_and_NF_Y_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-1119(08)00091-7 DB - PRIME DP - Unbound Medicine ER -