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Characterization and epitope mapping of monoclonal antibodies to the nucleocapsid protein of severe acute respiratory syndrome coronavirus.
Jpn J Vet Res. 2008 Feb; 55(4):115-27.JJ

Abstract

The sudden emergence of severe acute respiratory syndrome (SARS) at the end of 2002 resulted in 774 reported deaths from more than 8000 cases worldwide. As no effective vaccines or antiviral agents are available, the most effective measure to prevent the expansion of a SARS epidemic is the rapid diagnosis and isolation of SARS patients. To establish specific diagnostic methods, we generated nine clones of monoclonal antibodies to nucleocapsid protein (NP) of SARS-coronavirus (SARS-CoV). On immunofluorescent antibody assay and Western blotting analysis, none of the monoclonal antibodies showed cross-reactivity to authentic and recombinant NPs of human coronavirus (HCoV) 229E strain. To determine the region on the NP molecule where the monoclonal antibodies bind, we generated four truncated recombinant NPs and analyzed the reactivity between monoclonal antibodies and truncated NPs. Two monoclonal antibodies reacted with a truncated NP covering from amino acid residues 111 to 230, and seven reacted with another truncated NP covering from amino acid residues 221 to 340. Epitope mapping analysis indicated that monoclonal antibody SN5-25 recognized the amino acid sequence Q(245)TVTKK(250) On SARS-NP. Within the epitope, Q245, T246, V247, K249, and K250 appeared to form an essential motif for monoclonal antibody SN5-25 to bind. The information about binding sites and epitopes of monoclonal antibodies may be useful for the development of new diagnostic methods for SARS and for analyzing the function of N protein of SARS-CoV.

Authors+Show Affiliations

Laboratory of Public Health, Department of Environmental Veterinary Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan. kariwa@vetemed.hokudai.ac.jpNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18380153

Citation

Kariwa, Hiroaki, et al. "Characterization and Epitope Mapping of Monoclonal Antibodies to the Nucleocapsid Protein of Severe Acute Respiratory Syndrome Coronavirus." The Japanese Journal of Veterinary Research, vol. 55, no. 4, 2008, pp. 115-27.
Kariwa H, Noda H, Nakauchi M, et al. Characterization and epitope mapping of monoclonal antibodies to the nucleocapsid protein of severe acute respiratory syndrome coronavirus. Jpn J Vet Res. 2008;55(4):115-27.
Kariwa, H., Noda, H., Nakauchi, M., Ishizuka, M., Hashiguchi, K., Hashimoto, S., Yoshii, K., Asano, A., Agui, T., Kogaki, H., Kurano, Y., Uchida, Y., Fujii, N., Okada, M., & Takashima, I. (2008). Characterization and epitope mapping of monoclonal antibodies to the nucleocapsid protein of severe acute respiratory syndrome coronavirus. The Japanese Journal of Veterinary Research, 55(4), 115-27.
Kariwa H, et al. Characterization and Epitope Mapping of Monoclonal Antibodies to the Nucleocapsid Protein of Severe Acute Respiratory Syndrome Coronavirus. Jpn J Vet Res. 2008;55(4):115-27. PubMed PMID: 18380153.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization and epitope mapping of monoclonal antibodies to the nucleocapsid protein of severe acute respiratory syndrome coronavirus. AU - Kariwa,Hiroaki, AU - Noda,Hiroshi, AU - Nakauchi,Mina, AU - Ishizuka,Mariko, AU - Hashiguchi,Kazuaki, AU - Hashimoto,Shingo, AU - Yoshii,Kentaro, AU - Asano,Atsushi, AU - Agui,Takashi, AU - Kogaki,Hiroyuki, AU - Kurano,Yoshihiro, AU - Uchida,Yoshiaki, AU - Fujii,Nobuyuki, AU - Okada,Masahisa, AU - Takashima,Ikuo, PY - 2008/4/3/pubmed PY - 2008/5/28/medline PY - 2008/4/3/entrez SP - 115 EP - 27 JF - The Japanese journal of veterinary research JO - Jpn J Vet Res VL - 55 IS - 4 N2 - The sudden emergence of severe acute respiratory syndrome (SARS) at the end of 2002 resulted in 774 reported deaths from more than 8000 cases worldwide. As no effective vaccines or antiviral agents are available, the most effective measure to prevent the expansion of a SARS epidemic is the rapid diagnosis and isolation of SARS patients. To establish specific diagnostic methods, we generated nine clones of monoclonal antibodies to nucleocapsid protein (NP) of SARS-coronavirus (SARS-CoV). On immunofluorescent antibody assay and Western blotting analysis, none of the monoclonal antibodies showed cross-reactivity to authentic and recombinant NPs of human coronavirus (HCoV) 229E strain. To determine the region on the NP molecule where the monoclonal antibodies bind, we generated four truncated recombinant NPs and analyzed the reactivity between monoclonal antibodies and truncated NPs. Two monoclonal antibodies reacted with a truncated NP covering from amino acid residues 111 to 230, and seven reacted with another truncated NP covering from amino acid residues 221 to 340. Epitope mapping analysis indicated that monoclonal antibody SN5-25 recognized the amino acid sequence Q(245)TVTKK(250) On SARS-NP. Within the epitope, Q245, T246, V247, K249, and K250 appeared to form an essential motif for monoclonal antibody SN5-25 to bind. The information about binding sites and epitopes of monoclonal antibodies may be useful for the development of new diagnostic methods for SARS and for analyzing the function of N protein of SARS-CoV. SN - 0047-1917 UR - https://www.unboundmedicine.com/medline/citation/18380153/Characterization_and_epitope_mapping_of_monoclonal_antibodies_to_the_nucleocapsid_protein_of_severe_acute_respiratory_syndrome_coronavirus_ L2 - http://www.medicalonline.jp/meteo_linkout.php?issn=0047-1917&volume=55&issue=4&spage=115 DB - PRIME DP - Unbound Medicine ER -