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Effects of dietary supplementation with fish oil on in vivo production of inflammatory mediators in clinically normal dogs.
Am J Vet Res. 2008 Apr; 69(4):486-93.AJ

Abstract

OBJECTIVE

To evaluate the effect of diets enriched with eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) on in vivo production of interleukin (IL)-1, IL-6, tumor necrosis factor (TNF)-alpha, prostaglandin E2 (PGE2), and platelet-activating factor (PAF) in dogs.

ANIMALS

15 young healthy dogs.

PROCEDURES

Dogs were randomly allocated to receive an isocaloric ration supplemented with sunflower oil (n=5), fish oil (5), or fish oil plus vitamin E (5) for 12 weeks. At week 12, in vivo production of inflammatory mediators was evaluated in serum at multiple time points for 6 hours following stimulation with IV administration of lipopolysaccharide (LPS).

RESULTS

Serum activity or concentration (area under the curve) of IL-1, IL-6, and PGE2 significantly increased after LPS injection in all groups but to a lesser extent in dogs receiving the fish oil diet, compared with results for dogs receiving the sunflower oil diet. Serum activity of TNF-alpha and PAF concentration also increased significantly after LPS injection in all groups but did not differ significantly among groups.

CONCLUSIONS AND CLINICAL RELEVANCE

A fish oil-enriched diet consisting of 1.75 g of EPA/kg of diet and 2.2 g of DHA/kg of diet (dry-matter basis) with an n-6:n-3 fatty acid ratio of 3.4:1 was associated with significant reductions in serum PGE2 concentrations and IL-1 and IL-6 activities. Results supported the use of EPA- and DHA-enriched diets as part of antiinflammatory treatments for dogs with chronic inflammatory diseases. Additional studies in affected dogs are warranted to further evaluate beneficial anti-inflammatory effects of EPA- and DHA-enriched diets.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

LeBlanc CJ
Department of Pathobiological Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA 70803, USA.
Horohov DW
No affiliation info available
Bauer JE
No affiliation info available
Hosgood G
No affiliation info available
Mauldin GE
No affiliation info available

MeSH

AnimalsArea Under CurveCytokinesDietary SupplementsDinoprostoneDocosahexaenoic AcidsDogsEicosapentaenoic AcidFemaleInterleukin-1Interleukin-6MalePlatelet Activating FactorRandom AllocationTumor Necrosis Factor-alphaVitamin E

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18380580

Citation

LeBlanc, Casey J., et al. "Effects of Dietary Supplementation With Fish Oil On in Vivo Production of Inflammatory Mediators in Clinically Normal Dogs." American Journal of Veterinary Research, vol. 69, no. 4, 2008, pp. 486-93.
LeBlanc CJ, Horohov DW, Bauer JE, et al. Effects of dietary supplementation with fish oil on in vivo production of inflammatory mediators in clinically normal dogs. Am J Vet Res. 2008;69(4):486-93.
LeBlanc, C. J., Horohov, D. W., Bauer, J. E., Hosgood, G., & Mauldin, G. E. (2008). Effects of dietary supplementation with fish oil on in vivo production of inflammatory mediators in clinically normal dogs. American Journal of Veterinary Research, 69(4), 486-93. https://doi.org/10.2460/ajvr.69.4.486
LeBlanc CJ, et al. Effects of Dietary Supplementation With Fish Oil On in Vivo Production of Inflammatory Mediators in Clinically Normal Dogs. Am J Vet Res. 2008;69(4):486-93. PubMed PMID: 18380580.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of dietary supplementation with fish oil on in vivo production of inflammatory mediators in clinically normal dogs. AU - LeBlanc,Casey J, AU - Horohov,David W, AU - Bauer,John E, AU - Hosgood,Giselle, AU - Mauldin,Glenna E, PY - 2008/4/3/pubmed PY - 2008/6/27/medline PY - 2008/4/3/entrez SP - 486 EP - 93 JF - American journal of veterinary research JO - Am J Vet Res VL - 69 IS - 4 N2 - OBJECTIVE: To evaluate the effect of diets enriched with eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) on in vivo production of interleukin (IL)-1, IL-6, tumor necrosis factor (TNF)-alpha, prostaglandin E2 (PGE2), and platelet-activating factor (PAF) in dogs. ANIMALS: 15 young healthy dogs. PROCEDURES: Dogs were randomly allocated to receive an isocaloric ration supplemented with sunflower oil (n=5), fish oil (5), or fish oil plus vitamin E (5) for 12 weeks. At week 12, in vivo production of inflammatory mediators was evaluated in serum at multiple time points for 6 hours following stimulation with IV administration of lipopolysaccharide (LPS). RESULTS: Serum activity or concentration (area under the curve) of IL-1, IL-6, and PGE2 significantly increased after LPS injection in all groups but to a lesser extent in dogs receiving the fish oil diet, compared with results for dogs receiving the sunflower oil diet. Serum activity of TNF-alpha and PAF concentration also increased significantly after LPS injection in all groups but did not differ significantly among groups. CONCLUSIONS AND CLINICAL RELEVANCE: A fish oil-enriched diet consisting of 1.75 g of EPA/kg of diet and 2.2 g of DHA/kg of diet (dry-matter basis) with an n-6:n-3 fatty acid ratio of 3.4:1 was associated with significant reductions in serum PGE2 concentrations and IL-1 and IL-6 activities. Results supported the use of EPA- and DHA-enriched diets as part of antiinflammatory treatments for dogs with chronic inflammatory diseases. Additional studies in affected dogs are warranted to further evaluate beneficial anti-inflammatory effects of EPA- and DHA-enriched diets. SN - 0002-9645 UR - https://www.unboundmedicine.com/medline/citation/18380580/Effects_of_dietary_supplementation_with_fish_oil_on_in_vivo_production_of_inflammatory_mediators_in_clinically_normal_dogs_ DB - PRIME DP - Unbound Medicine ER -