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An Outbreak of Human Coronavirus OC43 Infection and Serological Cross-reactivity with SARS Coronavirus.
Can J Infect Dis Med Microbiol. 2006 Nov; 17(6):330-6.CJ

Abstract

BACKGROUND

In summer 2003, a respiratory outbreak was investigated in British Columbia, during which nucleic acid tests and serology unexpectedly indicated reactivity for severe acute respiratory syndrome coronavirus (SARS-CoV).

METHODS

Cases at a care facility were epidemiologically characterized and sequentially investigated for conventional agents of respiratory infection, SARS-CoV and other human CoVs. Serological cross-reactivity between SARS-CoV and human CoV-OC43 (HCoV-OC43) was investigated by peptide spot assay.

RESULTS

Ninety-five of 142 residents (67%) and 53 of 160 staff members (33%) experienced symptoms of respiratory infection. Symptomatic residents experienced cough (66%), fever (21%) and pneumonia (12%). Eight residents died, six with pneumonia. No staff members developed pneumonia. Findings on reverse transcriptase-polymerase chain reaction assays for SARS-CoV at a national reference laboratory were suspected to represent false positives, but this was confounded by concurrent identification of antibody to N protein on serology. Subsequent testing by reverse transcriptase-polymerase chain reaction confirmed HCoV-OC43 infection. Convalescent serology ruled out SARS. Notably, sera demonstrated cross-reactivity against nucleocapsid peptide sequences common to HCoV-OC43 and SARS-CoV.

CONCLUSIONS

These findings underscore the virulence of human CoV-OC43 in elderly populations and confirm that cross-reactivity to antibody against nucleocapsid proteins from these viruses must be considered when interpreting serological tests for SARS-CoV.

Authors+Show Affiliations

University of British Columbia Centre for Disease Control.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

18382647

Citation

Patrick, David M., et al. "An Outbreak of Human Coronavirus OC43 Infection and Serological Cross-reactivity With SARS Coronavirus." The Canadian Journal of Infectious Diseases & Medical Microbiology = Journal Canadien Des Maladies Infectieuses Et De La Microbiologie Medicale, vol. 17, no. 6, 2006, pp. 330-6.
Patrick DM, Petric M, Skowronski DM, et al. An Outbreak of Human Coronavirus OC43 Infection and Serological Cross-reactivity with SARS Coronavirus. The Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale. 2006;17(6):330-6.
Patrick, D. M., Petric, M., Skowronski, D. M., Guasparini, R., Booth, T. F., Krajden, M., McGeer, P., Bastien, N., Gustafson, L., Dubord, J., Macdonald, D., David, S. T., Srour, L. F., Parker, R., Andonov, A., Isaac-Renton, J., Loewen, N., McNabb, G., McNabb, A., ... Brunham, R. C. (2006). An Outbreak of Human Coronavirus OC43 Infection and Serological Cross-reactivity with SARS Coronavirus. The Canadian Journal of Infectious Diseases & Medical Microbiology = Journal Canadien Des Maladies Infectieuses Et De La Microbiologie Medicale, 17(6), 330-6.
Patrick DM, et al. An Outbreak of Human Coronavirus OC43 Infection and Serological Cross-reactivity With SARS Coronavirus. The Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale. 2006;17(6):330-6. PubMed PMID: 18382647.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - An Outbreak of Human Coronavirus OC43 Infection and Serological Cross-reactivity with SARS Coronavirus. AU - Patrick,David M, AU - Petric,Martin, AU - Skowronski,Danuta M, AU - Guasparini,Roland, AU - Booth,Timothy F, AU - Krajden,Mel, AU - McGeer,Patrick, AU - Bastien,Nathalie, AU - Gustafson,Larry, AU - Dubord,Janet, AU - Macdonald,Diane, AU - David,Samara T, AU - Srour,Leila F, AU - Parker,Robert, AU - Andonov,Anton, AU - Isaac-Renton,Judith, AU - Loewen,Nadine, AU - McNabb,Gail, AU - McNabb,Alan, AU - Goh,Swee-Han, AU - Henwick,Scott, AU - Astell,Caroline, AU - Guo,Jian Ping, AU - Drebot,Michael, AU - Tellier,Raymond, AU - Plummer,Francis, AU - Brunham,Robert C, PY - 2006/08/04/received PY - 2006/09/22/accepted PY - 2008/4/3/pubmed PY - 2008/4/3/medline PY - 2008/4/3/entrez KW - Coronavirus KW - Human coronavirus OC43 KW - Outbreak KW - Respiratory infection KW - SARS coronavirus KW - SARS-CoV KW - Severe acute respiratory syndrome SP - 330 EP - 6 JF - The Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale VL - 17 IS - 6 N2 - BACKGROUND: In summer 2003, a respiratory outbreak was investigated in British Columbia, during which nucleic acid tests and serology unexpectedly indicated reactivity for severe acute respiratory syndrome coronavirus (SARS-CoV). METHODS: Cases at a care facility were epidemiologically characterized and sequentially investigated for conventional agents of respiratory infection, SARS-CoV and other human CoVs. Serological cross-reactivity between SARS-CoV and human CoV-OC43 (HCoV-OC43) was investigated by peptide spot assay. RESULTS: Ninety-five of 142 residents (67%) and 53 of 160 staff members (33%) experienced symptoms of respiratory infection. Symptomatic residents experienced cough (66%), fever (21%) and pneumonia (12%). Eight residents died, six with pneumonia. No staff members developed pneumonia. Findings on reverse transcriptase-polymerase chain reaction assays for SARS-CoV at a national reference laboratory were suspected to represent false positives, but this was confounded by concurrent identification of antibody to N protein on serology. Subsequent testing by reverse transcriptase-polymerase chain reaction confirmed HCoV-OC43 infection. Convalescent serology ruled out SARS. Notably, sera demonstrated cross-reactivity against nucleocapsid peptide sequences common to HCoV-OC43 and SARS-CoV. CONCLUSIONS: These findings underscore the virulence of human CoV-OC43 in elderly populations and confirm that cross-reactivity to antibody against nucleocapsid proteins from these viruses must be considered when interpreting serological tests for SARS-CoV. SN - 1712-9532 UR - https://www.unboundmedicine.com/medline/citation/18382647/An_Outbreak_of_Human_Coronavirus_OC43_Infection_and_Serological_Cross_reactivity_with_SARS_Coronavirus_ L2 - https://doi.org/10.1155/2006/152612 DB - PRIME DP - Unbound Medicine ER -
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