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Capillary electrophoretic separation of biologically active amines and acids using nanoparticle-coated capillaries.
Electrophoresis. 2008 May; 29(9):1942-51.E

Abstract

This manuscript describes dynamic coating of capillaries with poly(L-lysine) (PLL) and silica nanoparticles (SiO2 NPs) and use of the as-prepared capillaries for the separation of biogenic amines and acids by CE in conjunction with LIF detection. The directions of EOF are controlled by varying the outmost layer of the capillaries with PLL and SiO2 NPs, respectively. Over the pH range 3.0-5.0, the (PLL-SiO2NP)n-PLL capillaries have an EOF toward the anodic end and are more suitable for the separation of acids with respect to speed, while the (PLL-SiO2NP)n capillaries have an EOF toward the cathodic end and are more suitable for the separation of biogenic amines regarding speed and sensitivity. The separations of standard solutions containing five amines and two acids by CE with LIF detection using (PLL-SiO2NP)2-PLL and (PLL-SiO2NP)3 capillaries were accomplished within 10 and 7 min, providing plate numbers of 3.8 and 5.0x10(4) plates/m for 5-hydroxytryptamine (5-HT), respectively. The LODs for 5-HT and 5-hydroxyindole-3-acetic acid (5-HIAA) are 32 and 2 nM and 0.2 and 1.5 nM when using the (PLL-SiO2NP)2-PLL and (PLL-SiO2NP)3 capillaries, respectively. Identification and quantification of 5-HIAA, homovanillic acid, and DL-vanillomandelic acid in urine samples from a male before and after drinking green tea were tested to validate practicality of the present approach. The results show that the (PLL-SiO2NP)2-PLL capillary provides greater resolving power, while the (PLL-SiO2NP)3 capillary provides better sensitivity, higher efficiency, and longer durability for the separation of the amines and acids.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Huang YF
Department of Chemistry, National Taiwan University, Taipei, Taiwan.
Chiang CK
No affiliation info available
Lin YW
No affiliation info available
Liu K
No affiliation info available
Hu CC
No affiliation info available
Bair MJ
No affiliation info available
Chang HT
No affiliation info available

MeSH

Biogenic AminesElectrophoresis, CapillaryEpinephrineFluorescenceHomovanillic AcidHumansHydrogen-Ion ConcentrationHydroxyindoleacetic AcidLasersMaleNanoparticlesPolylysineReproducibility of ResultsSensitivity and SpecificitySerotoninSilicon DioxideTeaTryptaminesTryptophan

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18383029

Citation

Huang, Yu-Fen, et al. "Capillary Electrophoretic Separation of Biologically Active Amines and Acids Using Nanoparticle-coated Capillaries." Electrophoresis, vol. 29, no. 9, 2008, pp. 1942-51.
Huang YF, Chiang CK, Lin YW, et al. Capillary electrophoretic separation of biologically active amines and acids using nanoparticle-coated capillaries. Electrophoresis. 2008;29(9):1942-51.
Huang, Y. F., Chiang, C. K., Lin, Y. W., Liu, K., Hu, C. C., Bair, M. J., & Chang, H. T. (2008). Capillary electrophoretic separation of biologically active amines and acids using nanoparticle-coated capillaries. Electrophoresis, 29(9), 1942-51. https://doi.org/10.1002/elps.200700534
Huang YF, et al. Capillary Electrophoretic Separation of Biologically Active Amines and Acids Using Nanoparticle-coated Capillaries. Electrophoresis. 2008;29(9):1942-51. PubMed PMID: 18383029.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Capillary electrophoretic separation of biologically active amines and acids using nanoparticle-coated capillaries. AU - Huang,Yu-Fen, AU - Chiang,Cheng-Kang, AU - Lin,Yang-Wei, AU - Liu,Kungtien, AU - Hu,Chou-Chen, AU - Bair,Ming-Jong, AU - Chang,Huan-Tsung, PY - 2008/4/3/pubmed PY - 2008/7/4/medline PY - 2008/4/3/entrez SP - 1942 EP - 51 JF - Electrophoresis JO - Electrophoresis VL - 29 IS - 9 N2 - This manuscript describes dynamic coating of capillaries with poly(L-lysine) (PLL) and silica nanoparticles (SiO2 NPs) and use of the as-prepared capillaries for the separation of biogenic amines and acids by CE in conjunction with LIF detection. The directions of EOF are controlled by varying the outmost layer of the capillaries with PLL and SiO2 NPs, respectively. Over the pH range 3.0-5.0, the (PLL-SiO2NP)n-PLL capillaries have an EOF toward the anodic end and are more suitable for the separation of acids with respect to speed, while the (PLL-SiO2NP)n capillaries have an EOF toward the cathodic end and are more suitable for the separation of biogenic amines regarding speed and sensitivity. The separations of standard solutions containing five amines and two acids by CE with LIF detection using (PLL-SiO2NP)2-PLL and (PLL-SiO2NP)3 capillaries were accomplished within 10 and 7 min, providing plate numbers of 3.8 and 5.0x10(4) plates/m for 5-hydroxytryptamine (5-HT), respectively. The LODs for 5-HT and 5-hydroxyindole-3-acetic acid (5-HIAA) are 32 and 2 nM and 0.2 and 1.5 nM when using the (PLL-SiO2NP)2-PLL and (PLL-SiO2NP)3 capillaries, respectively. Identification and quantification of 5-HIAA, homovanillic acid, and DL-vanillomandelic acid in urine samples from a male before and after drinking green tea were tested to validate practicality of the present approach. The results show that the (PLL-SiO2NP)2-PLL capillary provides greater resolving power, while the (PLL-SiO2NP)3 capillary provides better sensitivity, higher efficiency, and longer durability for the separation of the amines and acids. SN - 0173-0835 UR - https://www.unboundmedicine.com/medline/citation/18383029/Capillary_electrophoretic_separation_of_biologically_active_amines_and_acids_using_nanoparticle_coated_capillaries_ DB - PRIME DP - Unbound Medicine ER -
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