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Protective effects of glucagon-like peptide 2 on intestinal ischemia-reperfusion rats.
Microsurgery. 2008; 28(4):285-90.M

Abstract

Our objective was to evaluate the protective effects of glucagon-like peptide 2 (GLP-2) on intestinal ischemia/reperfusion (I/R) rats. Thirty-two rats were randomly assigned to four experimental groups, each of 8: Group A, sham rats underwent laparotomy only, without superior mesenteric artery (SMA) occlusion; Group B, I/R animals underwent laparotomy and occlusion of the SMA for 60 minutes followed by 120 minutes of reperfusion; Group C, I/R animals underwent intestinal I/R, and received pretreatment with GLP-2 for 3 days preoperatively; and Group D, I/R animals underwent intestinal I/R, received pretreatment with GLP-2 as above, and during the reperfusion phase were injected intravenously with GLP-2. After the reperfusion of intestinal ischemia, samples of intestinal mucosa, mesenteric lymph nodes (MLN) and blood were prepared for determination. In the pretreatment rats with GLP-2 (group C), Chiu's scores, bacterial colony counts, serum D-lactate, intestinal mucosal MDA and ET-1, and serum endotoxin, TNF-alpha and IL-6 were significantly reduced compared with intestinal I/R rats (group B). Administration of GLP-2 during the reperfusion phase following pretreatment (group D) showed further protective effects in comparison with the pretreatment rats (group C). We conclude that treatment with GLP-2 attenuates intestinal I/R injury, reduces bacterial translocation, inhibits the release of oxygen free radicals and ET-1, and may well inhibit the production of proinflammatory cytokines.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Zhang W
Research Institute of General Surgery, Jinling Hospital, Medical School of Nanjing University, Nanjing, People's Republic of China.
Zhu W
No affiliation info available
Zhang J
No affiliation info available
Li N
No affiliation info available
Li J
No affiliation info available

MeSH

AnimalsDisease Models, AnimalDrug Administration ScheduleEndothelin-1EndotoxinsGlucagon-Like Peptide 2Interleukin-6Intestinal MucosaIschemic PreconditioningMaleMalondialdehydeRandom AllocationRatsRats, Sprague-DawleyReperfusion InjuryTumor Necrosis Factor-alpha

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

18383347

Citation

Zhang, Wei, et al. "Protective Effects of Glucagon-like Peptide 2 On Intestinal Ischemia-reperfusion Rats." Microsurgery, vol. 28, no. 4, 2008, pp. 285-90.
Zhang W, Zhu W, Zhang J, et al. Protective effects of glucagon-like peptide 2 on intestinal ischemia-reperfusion rats. Microsurgery. 2008;28(4):285-90.
Zhang, W., Zhu, W., Zhang, J., Li, N., & Li, J. (2008). Protective effects of glucagon-like peptide 2 on intestinal ischemia-reperfusion rats. Microsurgery, 28(4), 285-90. https://doi.org/10.1002/micr.20491
Zhang W, et al. Protective Effects of Glucagon-like Peptide 2 On Intestinal Ischemia-reperfusion Rats. Microsurgery. 2008;28(4):285-90. PubMed PMID: 18383347.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protective effects of glucagon-like peptide 2 on intestinal ischemia-reperfusion rats. AU - Zhang,Wei, AU - Zhu,Weiming, AU - Zhang,Jian, AU - Li,Ning, AU - Li,Jieshou, PY - 2008/4/3/pubmed PY - 2008/6/24/medline PY - 2008/4/3/entrez SP - 285 EP - 90 JF - Microsurgery JO - Microsurgery VL - 28 IS - 4 N2 - Our objective was to evaluate the protective effects of glucagon-like peptide 2 (GLP-2) on intestinal ischemia/reperfusion (I/R) rats. Thirty-two rats were randomly assigned to four experimental groups, each of 8: Group A, sham rats underwent laparotomy only, without superior mesenteric artery (SMA) occlusion; Group B, I/R animals underwent laparotomy and occlusion of the SMA for 60 minutes followed by 120 minutes of reperfusion; Group C, I/R animals underwent intestinal I/R, and received pretreatment with GLP-2 for 3 days preoperatively; and Group D, I/R animals underwent intestinal I/R, received pretreatment with GLP-2 as above, and during the reperfusion phase were injected intravenously with GLP-2. After the reperfusion of intestinal ischemia, samples of intestinal mucosa, mesenteric lymph nodes (MLN) and blood were prepared for determination. In the pretreatment rats with GLP-2 (group C), Chiu's scores, bacterial colony counts, serum D-lactate, intestinal mucosal MDA and ET-1, and serum endotoxin, TNF-alpha and IL-6 were significantly reduced compared with intestinal I/R rats (group B). Administration of GLP-2 during the reperfusion phase following pretreatment (group D) showed further protective effects in comparison with the pretreatment rats (group C). We conclude that treatment with GLP-2 attenuates intestinal I/R injury, reduces bacterial translocation, inhibits the release of oxygen free radicals and ET-1, and may well inhibit the production of proinflammatory cytokines. SN - 1098-2752 UR - https://www.unboundmedicine.com/medline/citation/18383347/Protective_effects_of_glucagon_like_peptide_2_on_intestinal_ischemia_reperfusion_rats_ DB - PRIME DP - Unbound Medicine ER -