Tags

Type your tag names separated by a space and hit enter

Human tissue transglutaminase antibody screening by immunochromatographic line immunoassay for early diagnosis of celiac disease in Turkish children.

Abstract

BACKGROUND/AIMS

Celiac disease has a large prevalence worldwide. There are a limited number of comparable epidemiological data for celiac disease in Turkey. The aim of this preliminary study was to determine the prevalence of celiac disease in a sample of 1000 Turkish children by a novel, simple, and visual one-step immunoassay screening test.

METHODS

This prospective study consisted of 1000 serum samples from apparently healthy children and children with disorders other than celiac disease aged between 2-18 years who presented as outpatients at Ankara University, Faculty of Medicine, Department of Pediatrics. Sera were tested for IgA-class antibodies against human tissue transglutaminase and gliadin by rapid immunochromatographic line immunoassay. Endomysial antibody IgA against human tissue transglutaminase and AGA IgA/IgG were also tested by ELISA as a second step when the result of the screening test was positive. Small bowel biopsy was recommended to all the children with positive anti-tissue transglutaminase and/or endomysial antibody results.

RESULTS

Ten of the 1000 individuals (1%) had positive antibody screening test to human tissue transglutaminase. All tissue transglutaminase-positive samples revealed good correlation with endomysial antibody by ELISA method. Subsequently small bowel biopsy was performed in all serology-positive cases. Biopsy results confirmed a diagnosis of celiac disease in nine cases. The prevalence of biopsy- proven celiac disease was 1:111 (0.9%).

CONCLUSIONS

Determination of anti-tissue transglutaminase antibodies by simple visual system for celiac disease appeared to be as reliable as the ELISA system. It is easy to perform and interpret, cost-effective, and rapid, as pointed out in other previous studies, as a screening test in large population-based studies. The prevalence of celiac disease in the overall sample of Turkish children (1:111 or 0.9%) in this preliminary study is similar to that reported in European and Middle Eastern countries and the United States.

Links

  • FREE Publisher Full Text
  • Authors+Show Affiliations

    ,

    Department of Pediatrics, Division of Pediatric Gastroenterology, Faculty of Medicine, Kirikkale University, Kirikkale. fulya.gulerman@gmail.com

    , , , ,

    Source

    MeSH

    Adolescent
    Celiac Disease
    Child
    Child, Preschool
    Chromatography
    Duodenum
    Early Diagnosis
    Endoscopy, Digestive System
    Female
    Humans
    Immunoassay
    Immunoglobulin A
    Male
    Mass Screening
    Prevalence
    Prospective Studies
    Transglutaminases
    Turkey

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    18386235

    Citation

    Demirçeken, Fulya G., et al. "Human Tissue Transglutaminase Antibody Screening By Immunochromatographic Line Immunoassay for Early Diagnosis of Celiac Disease in Turkish Children." The Turkish Journal of Gastroenterology : the Official Journal of Turkish Society of Gastroenterology, vol. 19, no. 1, 2008, pp. 14-21.
    Demirçeken FG, Kansu A, Kuloğlu Z, et al. Human tissue transglutaminase antibody screening by immunochromatographic line immunoassay for early diagnosis of celiac disease in Turkish children. Turk J Gastroenterol. 2008;19(1):14-21.
    Demirçeken, F. G., Kansu, A., Kuloğlu, Z., Girgin, N., Güriz, H., & Ensari, A. (2008). Human tissue transglutaminase antibody screening by immunochromatographic line immunoassay for early diagnosis of celiac disease in Turkish children. The Turkish Journal of Gastroenterology : the Official Journal of Turkish Society of Gastroenterology, 19(1), pp. 14-21.
    Demirçeken FG, et al. Human Tissue Transglutaminase Antibody Screening By Immunochromatographic Line Immunoassay for Early Diagnosis of Celiac Disease in Turkish Children. Turk J Gastroenterol. 2008;19(1):14-21. PubMed PMID: 18386235.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Human tissue transglutaminase antibody screening by immunochromatographic line immunoassay for early diagnosis of celiac disease in Turkish children. AU - Demirçeken,Fulya G, AU - Kansu,Aydan, AU - Kuloğlu,Zarife, AU - Girgin,Nurten, AU - Güriz,Haluk, AU - Ensari,Arzu, PY - 2008/4/4/pubmed PY - 2009/3/28/medline PY - 2008/4/4/entrez SP - 14 EP - 21 JF - The Turkish journal of gastroenterology : the official journal of Turkish Society of Gastroenterology JO - Turk J Gastroenterol VL - 19 IS - 1 N2 - BACKGROUND/AIMS: Celiac disease has a large prevalence worldwide. There are a limited number of comparable epidemiological data for celiac disease in Turkey. The aim of this preliminary study was to determine the prevalence of celiac disease in a sample of 1000 Turkish children by a novel, simple, and visual one-step immunoassay screening test. METHODS: This prospective study consisted of 1000 serum samples from apparently healthy children and children with disorders other than celiac disease aged between 2-18 years who presented as outpatients at Ankara University, Faculty of Medicine, Department of Pediatrics. Sera were tested for IgA-class antibodies against human tissue transglutaminase and gliadin by rapid immunochromatographic line immunoassay. Endomysial antibody IgA against human tissue transglutaminase and AGA IgA/IgG were also tested by ELISA as a second step when the result of the screening test was positive. Small bowel biopsy was recommended to all the children with positive anti-tissue transglutaminase and/or endomysial antibody results. RESULTS: Ten of the 1000 individuals (1%) had positive antibody screening test to human tissue transglutaminase. All tissue transglutaminase-positive samples revealed good correlation with endomysial antibody by ELISA method. Subsequently small bowel biopsy was performed in all serology-positive cases. Biopsy results confirmed a diagnosis of celiac disease in nine cases. The prevalence of biopsy- proven celiac disease was 1:111 (0.9%). CONCLUSIONS: Determination of anti-tissue transglutaminase antibodies by simple visual system for celiac disease appeared to be as reliable as the ELISA system. It is easy to perform and interpret, cost-effective, and rapid, as pointed out in other previous studies, as a screening test in large population-based studies. The prevalence of celiac disease in the overall sample of Turkish children (1:111 or 0.9%) in this preliminary study is similar to that reported in European and Middle Eastern countries and the United States. SN - 2148-5607 UR - https://www.unboundmedicine.com/medline/citation/18386235/Human_tissue_transglutaminase_antibody_screening_by_immunochromatographic_line_immunoassay_for_early_diagnosis_of_celiac_disease_in_Turkish_children_ L2 - http://www.turkjgastroenterol.org/eng/makale/3411/228/Full-Text DB - PRIME DP - Unbound Medicine ER -