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Microarray-based detection of dye-labeled DNA by SERRS using particles formed by enzymatic silver deposition.
Chemphyschem. 2008 Apr 21; 9(6):867-72.C

Abstract

The growing interest in DNA diagnostics is addressed today by microarrays with fluoresence detection. In our approach, we utilize spatially defined arrays of short oligonucleotides on a modified glass surface. Surface enhanced resonance Raman scattering (SERRS) is used to obtain molecularly specific spectra of the Raman-active dye-labeled DNA. Nanoparticles produced by enzymatic silver deposition are used as SERS-active substrate. They grow directly on the modified oligonucleotides and only in the spatially defined areas on the chip. Furthermore, they potentially offer several advantages for SERS detection. The nanoparticles are characterized and their ability for use as SERS- and SERRS-active substrate is estimated. Three different Raman-active dyes are investigated for their potential for involvement in sequence specific DNA analysis.

Authors+Show Affiliations

Jenaer Biochip Initiative, Institute of Physical Chemistry, Friedrich-Schiller Universität Jena, Helmholtzweg 4, 07745 Jena, Germany.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18386261

Citation

Hering, Katharina Konstanze, et al. "Microarray-based Detection of Dye-labeled DNA By SERRS Using Particles Formed By Enzymatic Silver Deposition." Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry, vol. 9, no. 6, 2008, pp. 867-72.
Hering KK, Möller R, Fritzsche W, et al. Microarray-based detection of dye-labeled DNA by SERRS using particles formed by enzymatic silver deposition. Chemphyschem. 2008;9(6):867-72.
Hering, K. K., Möller, R., Fritzsche, W., & Popp, J. (2008). Microarray-based detection of dye-labeled DNA by SERRS using particles formed by enzymatic silver deposition. Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry, 9(6), 867-72. https://doi.org/10.1002/cphc.200700591
Hering KK, et al. Microarray-based Detection of Dye-labeled DNA By SERRS Using Particles Formed By Enzymatic Silver Deposition. Chemphyschem. 2008 Apr 21;9(6):867-72. PubMed PMID: 18386261.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Microarray-based detection of dye-labeled DNA by SERRS using particles formed by enzymatic silver deposition. AU - Hering,Katharina Konstanze, AU - Möller,Robert, AU - Fritzsche,Wolfgang, AU - Popp,Jürgen, PY - 2008/4/4/pubmed PY - 2008/6/17/medline PY - 2008/4/4/entrez SP - 867 EP - 72 JF - Chemphyschem : a European journal of chemical physics and physical chemistry JO - Chemphyschem VL - 9 IS - 6 N2 - The growing interest in DNA diagnostics is addressed today by microarrays with fluoresence detection. In our approach, we utilize spatially defined arrays of short oligonucleotides on a modified glass surface. Surface enhanced resonance Raman scattering (SERRS) is used to obtain molecularly specific spectra of the Raman-active dye-labeled DNA. Nanoparticles produced by enzymatic silver deposition are used as SERS-active substrate. They grow directly on the modified oligonucleotides and only in the spatially defined areas on the chip. Furthermore, they potentially offer several advantages for SERS detection. The nanoparticles are characterized and their ability for use as SERS- and SERRS-active substrate is estimated. Three different Raman-active dyes are investigated for their potential for involvement in sequence specific DNA analysis. SN - 1439-7641 UR - https://www.unboundmedicine.com/medline/citation/18386261/Microarray_based_detection_of_dye_labeled_DNA_by_SERRS_using_particles_formed_by_enzymatic_silver_deposition_ L2 - https://doi.org/10.1002/cphc.200700591 DB - PRIME DP - Unbound Medicine ER -