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Hesperidin, hesperidin methyl chalone and phellopterin from Poncirus trifoliata (Rutaceae) differentially regulate the expression of adhesion molecules in tumor necrosis factor-alpha-stimulated human umbilical vein endothelial cells.
Int Immunopharmacol. 2008 May; 8(5):670-8.II

Abstract

The fruits of Poncirus trifoliata (L.) are widely used in Oriental medicine to treat allergic inflammation. Recently, several active compounds including hesperidin, hesperidin methyl chalone and phellopterin from P. trifoliata (Rutaceae) were isolated and characterized. The goal of this study was to investigate the differential effect of hesperidin, hesperidin methyl chalone and phellopterin derived from P. trifoliata (Rutaceae) on the induction of intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) by TNF-alpha and the possible molecular mechanisms by which they differentially regulate ICAM-1 and VCAM-1 expressions. Stimulation of human umbilical vein endothelial cells (HUVECs) with TNF-alpha resulted in the increase of ICAM-1 and VCAM-1 expressions, while pretreatment with the three components completely inhibited VCAM-1 expression in a dose-dependent manner but had no effect on ICAM-1 expression. All three compounds failed to block TNF-alpha-induced phosphorylation of ERK1/2, which is involved in regulating ICAM-1 production by TNF-alpha. Furthermore, they efficiently inhibited the phosphorylation of Akt and PKC, suggesting that Akt or PKC pathways are an important target by which these compounds regulate TNF-alpha-induced VCAM-1 but not ICAM-1. Additionally, treatment with these chemicals also inhibited U937 monocyte adhesion to HUVECs stimulated with TNF-alpha. Interestingly, the inhibitory effect of hesperidin, hesperidin methyl chalone and phellopterin on monocyte adhesion to HUVECs was recapitulated by transfecting cells with VCAM-1 siRNA. Taken together, hesperidin, hesperidin methyl chalone and phellopterin reduce TNF-alpha-induced VCAM-1 expression through regulation of the Akt and PKC pathway, which contributes to inhibit the adhesion of monocytes to endothelium.

Authors+Show Affiliations

Department of Pharmacology, School of Medicine, Institute of Health Sciences, Gyeongsang National University, Jinju, Republic of Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18387509

Citation

Nizamutdinova, Irina Tsoy, et al. "Hesperidin, Hesperidin Methyl Chalone and Phellopterin From Poncirus Trifoliata (Rutaceae) Differentially Regulate the Expression of Adhesion Molecules in Tumor Necrosis Factor-alpha-stimulated Human Umbilical Vein Endothelial Cells." International Immunopharmacology, vol. 8, no. 5, 2008, pp. 670-8.
Nizamutdinova IT, Jeong JJ, Xu GH, et al. Hesperidin, hesperidin methyl chalone and phellopterin from Poncirus trifoliata (Rutaceae) differentially regulate the expression of adhesion molecules in tumor necrosis factor-alpha-stimulated human umbilical vein endothelial cells. Int Immunopharmacol. 2008;8(5):670-8.
Nizamutdinova, I. T., Jeong, J. J., Xu, G. H., Lee, S. H., Kang, S. S., Kim, Y. S., Chang, K. C., & Kim, H. J. (2008). Hesperidin, hesperidin methyl chalone and phellopterin from Poncirus trifoliata (Rutaceae) differentially regulate the expression of adhesion molecules in tumor necrosis factor-alpha-stimulated human umbilical vein endothelial cells. International Immunopharmacology, 8(5), 670-8. https://doi.org/10.1016/j.intimp.2008.01.011
Nizamutdinova IT, et al. Hesperidin, Hesperidin Methyl Chalone and Phellopterin From Poncirus Trifoliata (Rutaceae) Differentially Regulate the Expression of Adhesion Molecules in Tumor Necrosis Factor-alpha-stimulated Human Umbilical Vein Endothelial Cells. Int Immunopharmacol. 2008;8(5):670-8. PubMed PMID: 18387509.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Hesperidin, hesperidin methyl chalone and phellopterin from Poncirus trifoliata (Rutaceae) differentially regulate the expression of adhesion molecules in tumor necrosis factor-alpha-stimulated human umbilical vein endothelial cells. AU - Nizamutdinova,Irina Tsoy, AU - Jeong,Jae Ju, AU - Xu,Guang Hua, AU - Lee,Seung-Ho, AU - Kang,Sam Sik, AU - Kim,Yeong Shik, AU - Chang,Ki Churl, AU - Kim,Hye Jung, Y1 - 2008/02/08/ PY - 2007/10/15/received PY - 2007/12/18/revised PY - 2008/01/11/accepted PY - 2008/4/5/pubmed PY - 2008/7/23/medline PY - 2008/4/5/entrez SP - 670 EP - 8 JF - International immunopharmacology JO - Int Immunopharmacol VL - 8 IS - 5 N2 - The fruits of Poncirus trifoliata (L.) are widely used in Oriental medicine to treat allergic inflammation. Recently, several active compounds including hesperidin, hesperidin methyl chalone and phellopterin from P. trifoliata (Rutaceae) were isolated and characterized. The goal of this study was to investigate the differential effect of hesperidin, hesperidin methyl chalone and phellopterin derived from P. trifoliata (Rutaceae) on the induction of intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) by TNF-alpha and the possible molecular mechanisms by which they differentially regulate ICAM-1 and VCAM-1 expressions. Stimulation of human umbilical vein endothelial cells (HUVECs) with TNF-alpha resulted in the increase of ICAM-1 and VCAM-1 expressions, while pretreatment with the three components completely inhibited VCAM-1 expression in a dose-dependent manner but had no effect on ICAM-1 expression. All three compounds failed to block TNF-alpha-induced phosphorylation of ERK1/2, which is involved in regulating ICAM-1 production by TNF-alpha. Furthermore, they efficiently inhibited the phosphorylation of Akt and PKC, suggesting that Akt or PKC pathways are an important target by which these compounds regulate TNF-alpha-induced VCAM-1 but not ICAM-1. Additionally, treatment with these chemicals also inhibited U937 monocyte adhesion to HUVECs stimulated with TNF-alpha. Interestingly, the inhibitory effect of hesperidin, hesperidin methyl chalone and phellopterin on monocyte adhesion to HUVECs was recapitulated by transfecting cells with VCAM-1 siRNA. Taken together, hesperidin, hesperidin methyl chalone and phellopterin reduce TNF-alpha-induced VCAM-1 expression through regulation of the Akt and PKC pathway, which contributes to inhibit the adhesion of monocytes to endothelium. SN - 1567-5769 UR - https://www.unboundmedicine.com/medline/citation/18387509/Hesperidin_hesperidin_methyl_chalone_and_phellopterin_from_Poncirus_trifoliata__Rutaceae__differentially_regulate_the_expression_of_adhesion_molecules_in_tumor_necrosis_factor_alpha_stimulated_human_umbilical_vein_endothelial_cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1567-5769(08)00014-3 DB - PRIME DP - Unbound Medicine ER -