TY - JOUR T1 - A comparative study on cannabidiol-induced apoptosis in murine thymocytes and EL-4 thymoma cells. AU - Lee,Chi-Ya, AU - Wey,Shiaw-Pyng, AU - Liao,Mei-Hsiu, AU - Hsu,Wei-Lun, AU - Wu,Hsin-Ying, AU - Jan,Tong-Rong, Y1 - 2008/02/14/ PY - 2007/10/11/received PY - 2008/01/17/revised PY - 2008/01/17/accepted PY - 2008/4/5/pubmed PY - 2008/7/23/medline PY - 2008/4/5/entrez SP - 732 EP - 40 JF - International immunopharmacology JO - Int Immunopharmacol VL - 8 IS - 5 N2 - It has been shown that leukemia and glioma cells are sensitive to cannabidiol (CBD)-induced apoptosis, whereas primary monocytes and glia cells are relatively insensitive. In the current study, the cellular events and sensitivity to CBD-induced apoptosis between murine thymocytes and EL-4 thymoma cells were compared. Cannabidiol markedly induced apoptosis in a time- and concentration-related manner in both cells. The efficacy of CBD to induce apoptosis was comparable between the 2 types of T cells, whereas CBD induced apoptosis in thymocytes with a slightly greater potency than in EL4 cells. Time-course analyses revealed CBD-mediated apoptosis occurred earlier in EL-4 cells than that in thymocytes. An increased level of cellular reactive oxygen species (ROS) was detected in both cells with the peak response at 2 h post CBD treatment. Concordantly, CBD triggered a gradual diminishment in the cellular thiols. The presence of N-acetyl-L-cysteine (NAC), a precursor of glutathione, markedly attenuated the induction of apoptosis, and restored the diminished levels of cellular thiols. The results demonstrated that both thymocytes and EL-4 thymoma cells were susceptible to CBD-induced apoptosis and that ROS played a critical role in the apoptosis induction. SN - 1567-5769 UR - https://www.unboundmedicine.com/medline/citation/18387516/A_comparative_study_on_cannabidiol_induced_apoptosis_in_murine_thymocytes_and_EL_4_thymoma_cells_ DB - PRIME DP - Unbound Medicine ER -