Tags

Type your tag names separated by a space and hit enter

Therapeutic effect of magnesium lithospermate B on neointimal formation after balloon-induced vascular injury.
Eur J Pharmacol. 2008 May 31; 586(1-3):226-33.EJ

Abstract

Vascular smooth muscle cell (VSMC) proliferation and migration in response to platelet-derived growth factor (PDGF) play an important role in the development of atherosclerosis and restenosis. Recent evidence indicates that PDGF increases intracellular levels of reactive oxygen species in VSMCs and that both PDGF-induced VSMC proliferation and migration are reactive oxygen species-dependent. Danshen is a representative oriental medicine used for the treatment of vascular disease. Previously, we reported that magnesium lithospermate B, an active component of Danshen, is a potent antioxidant. Thus we investigated the therapeutic potential of magnesium lithospermate B in neointimal formation after carotid artery injury in rats along with its effects on the PDGF signaling pathway for stimulating VSMC proliferation and migration in vitro. PDGF is dimeric glycoprotein composed of two A or two B chains. In this study, we used PDGF-BB, which is one of the isoforms of PDGF (i.e., PDGF-AA, PDGF-BB, and PDGF-AB). Our results demonstrated that magnesium lithospermate B directly scavenged reactive oxygen species in a xanthine/xanthine oxidase system and reduced PDGF-BB-induced intracellular reactive oxygen species generation in VSMCs. In a rat carotid artery balloon injury model, magnesium lithospermate B treatment (10 mg/kg/day, i.p) showed a significant effect on the prevention of neointimal formation compared with vehicle treatment. In cultured VSMCs, magnesium lithospermate B significantly attenuated PDGF-BB-induced cell proliferation and migration as measured by 3-[4,5-dimethyl-2-thiazolyl]-2,5-diphenyl-2-tetrazolium bromide (MTT) assay and transwell migration assays, respectively. Further, magnesium lithospermate B inhibited PDGF-BB-induced phosphorylation of phospatidylinositol 3-kinase (PI3K)/Akt and mitogen-activated protein kinase (MAPK)/extracellular signal-regulated kinase (ERK) pathways by scavenging reactive oxygen species. Together, these data indicated that magnesium lithospermate B, a potent reactive oxygen species scavenger, prevented both injury-induced neointimal formation in vivo and PDGF-BB-induced VSMC proliferation and migration in vitro, suggesting that magnesium lithospermate B may be a promising agent to prevent atherosclerosis and restenosis following angioplasty.

Authors+Show Affiliations

Brain Korea 21 Project for Medical Science, Yonsei University, Seoul, Republic of Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18387604

Citation

Hur, Kyu Yeon, et al. "Therapeutic Effect of Magnesium Lithospermate B On Neointimal Formation After Balloon-induced Vascular Injury." European Journal of Pharmacology, vol. 586, no. 1-3, 2008, pp. 226-33.
Hur KY, Seo HJ, Kang ES, et al. Therapeutic effect of magnesium lithospermate B on neointimal formation after balloon-induced vascular injury. Eur J Pharmacol. 2008;586(1-3):226-33.
Hur, K. Y., Seo, H. J., Kang, E. S., Kim, S. H., Song, S., Kim, E. H., Lim, S., Choi, C., Heo, J. H., Hwang, K. C., Ahn, C. W., Cha, B. S., Jung, M., & Lee, H. C. (2008). Therapeutic effect of magnesium lithospermate B on neointimal formation after balloon-induced vascular injury. European Journal of Pharmacology, 586(1-3), 226-33. https://doi.org/10.1016/j.ejphar.2008.02.072
Hur KY, et al. Therapeutic Effect of Magnesium Lithospermate B On Neointimal Formation After Balloon-induced Vascular Injury. Eur J Pharmacol. 2008 May 31;586(1-3):226-33. PubMed PMID: 18387604.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Therapeutic effect of magnesium lithospermate B on neointimal formation after balloon-induced vascular injury. AU - Hur,Kyu Yeon, AU - Seo,Hye Jun, AU - Kang,Eun Seok, AU - Kim,Soo Hyun, AU - Song,Seungjeong, AU - Kim,Eun Hee, AU - Lim,Soyeon, AU - Choi,Chulhee, AU - Heo,Ji Hoe, AU - Hwang,Ki Chul, AU - Ahn,Chul Woo, AU - Cha,Bong Soo, AU - Jung,Mankil, AU - Lee,Hyun Chul, Y1 - 2008/02/29/ PY - 2007/08/14/received PY - 2008/01/30/revised PY - 2008/02/13/accepted PY - 2008/4/5/pubmed PY - 2008/9/3/medline PY - 2008/4/5/entrez SP - 226 EP - 33 JF - European journal of pharmacology JO - Eur. J. Pharmacol. VL - 586 IS - 1-3 N2 - Vascular smooth muscle cell (VSMC) proliferation and migration in response to platelet-derived growth factor (PDGF) play an important role in the development of atherosclerosis and restenosis. Recent evidence indicates that PDGF increases intracellular levels of reactive oxygen species in VSMCs and that both PDGF-induced VSMC proliferation and migration are reactive oxygen species-dependent. Danshen is a representative oriental medicine used for the treatment of vascular disease. Previously, we reported that magnesium lithospermate B, an active component of Danshen, is a potent antioxidant. Thus we investigated the therapeutic potential of magnesium lithospermate B in neointimal formation after carotid artery injury in rats along with its effects on the PDGF signaling pathway for stimulating VSMC proliferation and migration in vitro. PDGF is dimeric glycoprotein composed of two A or two B chains. In this study, we used PDGF-BB, which is one of the isoforms of PDGF (i.e., PDGF-AA, PDGF-BB, and PDGF-AB). Our results demonstrated that magnesium lithospermate B directly scavenged reactive oxygen species in a xanthine/xanthine oxidase system and reduced PDGF-BB-induced intracellular reactive oxygen species generation in VSMCs. In a rat carotid artery balloon injury model, magnesium lithospermate B treatment (10 mg/kg/day, i.p) showed a significant effect on the prevention of neointimal formation compared with vehicle treatment. In cultured VSMCs, magnesium lithospermate B significantly attenuated PDGF-BB-induced cell proliferation and migration as measured by 3-[4,5-dimethyl-2-thiazolyl]-2,5-diphenyl-2-tetrazolium bromide (MTT) assay and transwell migration assays, respectively. Further, magnesium lithospermate B inhibited PDGF-BB-induced phosphorylation of phospatidylinositol 3-kinase (PI3K)/Akt and mitogen-activated protein kinase (MAPK)/extracellular signal-regulated kinase (ERK) pathways by scavenging reactive oxygen species. Together, these data indicated that magnesium lithospermate B, a potent reactive oxygen species scavenger, prevented both injury-induced neointimal formation in vivo and PDGF-BB-induced VSMC proliferation and migration in vitro, suggesting that magnesium lithospermate B may be a promising agent to prevent atherosclerosis and restenosis following angioplasty. SN - 0014-2999 UR - https://www.unboundmedicine.com/medline/citation/18387604/Therapeutic_effect_of_magnesium_lithospermate_B_on_neointimal_formation_after_balloon_induced_vascular_injury_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0014-2999(08)00171-4 DB - PRIME DP - Unbound Medicine ER -