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Responses of chronically contaminated biofilms to short pulses of diuron. An experimental study simulating flooding events in a small river.
Aquat Toxicol. 2008 May 30; 87(4):252-63.AT

Abstract

An experimental study was undertaken to highlight the potential ecotoxicological impact of the herbicide diuron on biofilms during flooding events in a small river (Morcille) in the Beaujolais vineyard area (France). We investigated the responses of chronically contaminated biofilms exposed to short-term pulses (3 h) of diuron. Biofilms were grown in indoor microcosms that were either non-contaminated or exposed to low-level chronic contamination, and not exposed, or exposed to single or double pulses of two environmental concentrations (7 and 14 microg L(-1)) of diuron. Exposure to pollution and its impact on biofilms were assessed by measuring pesticide concentrations in biofilms, biomass parameters (chl a, AFDW), community structure (using 18S and 16S rDNA gene analysis by DGGE, and HPLC pigment analysis to target eukaryotes, bacteria and photoautotrophs, respectively) and by performing a physiological test. Control biofilms displayed very low diuron concentrations, whereas the herbicide was found in the contaminated biofilms. Nevertheless, diuron concentrations were not higher in the pulsed biofilms than in the non-pulsed ones. AFDW and chl ain vivo fluorescence increased in both microcosms during the experiment and biomass was higher in chronically exposed biofilms than in control ones. The impact on biomass was higher for the control double-pulsed biofilms than for the non-pulsed ones. Carbon incorporation by the chronically exposed biofilms was greater during the first 28 days of growth than during the first 28 days of growth in the control biofilms. Both single and double pulses inhibited carbon incorporation of all biofilm communities, especially of the control ones. Short-term inhibition of photosynthesis was never significantly different in exposed and non-exposed biofilms. Few differences in the pigment structure were found between chronically exposed and control biofilms, but pulses impacted on the pigment structure of all biofilm communities. Bacterial structural differences were observed between single-pulsed and non-pulsed biofilms, but not between double-pulsed and non-pulsed biofilms. The different pulses affected the eukaryotic community structures of the control biofilms, but not of the chronically exposed ones. Unlike the bacterial communities, the control eukaryotic communities were structurally different from the chronically exposed ones. This preliminary experimental study indicates that exposure to environmental concentrations of diuron and other agricultural contaminants and further exposure to diuron can have measurable effects on small river biofilm communities. The effects of a pulsed acute exposure to diuron on biofilms depended on whether the biofilms had previously been exposed to the same stressors or not.

Authors+Show Affiliations

CEMAGREF, UR Qualité des Eaux, 3 bis Quai Chauveau CP 220, 69336 Lyon Cedex 09, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18387680

Citation

Tlili, Ahmed, et al. "Responses of Chronically Contaminated Biofilms to Short Pulses of Diuron. an Experimental Study Simulating Flooding Events in a Small River." Aquatic Toxicology (Amsterdam, Netherlands), vol. 87, no. 4, 2008, pp. 252-63.
Tlili A, Dorigo U, Montuelle B, et al. Responses of chronically contaminated biofilms to short pulses of diuron. An experimental study simulating flooding events in a small river. Aquat Toxicol. 2008;87(4):252-63.
Tlili, A., Dorigo, U., Montuelle, B., Margoum, C., Carluer, N., Gouy, V., Bouchez, A., & Bérard, A. (2008). Responses of chronically contaminated biofilms to short pulses of diuron. An experimental study simulating flooding events in a small river. Aquatic Toxicology (Amsterdam, Netherlands), 87(4), 252-63. https://doi.org/10.1016/j.aquatox.2008.02.004
Tlili A, et al. Responses of Chronically Contaminated Biofilms to Short Pulses of Diuron. an Experimental Study Simulating Flooding Events in a Small River. Aquat Toxicol. 2008 May 30;87(4):252-63. PubMed PMID: 18387680.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Responses of chronically contaminated biofilms to short pulses of diuron. An experimental study simulating flooding events in a small river. AU - Tlili,Ahmed, AU - Dorigo,Ursula, AU - Montuelle,Bernard, AU - Margoum,Christelle, AU - Carluer,Nadia, AU - Gouy,Véronique, AU - Bouchez,Agnès, AU - Bérard,Annette, Y1 - 2008/02/19/ PY - 2007/11/13/received PY - 2008/01/30/revised PY - 2008/02/01/accepted PY - 2008/4/5/pubmed PY - 2008/7/17/medline PY - 2008/4/5/entrez SP - 252 EP - 63 JF - Aquatic toxicology (Amsterdam, Netherlands) JO - Aquat Toxicol VL - 87 IS - 4 N2 - An experimental study was undertaken to highlight the potential ecotoxicological impact of the herbicide diuron on biofilms during flooding events in a small river (Morcille) in the Beaujolais vineyard area (France). We investigated the responses of chronically contaminated biofilms exposed to short-term pulses (3 h) of diuron. Biofilms were grown in indoor microcosms that were either non-contaminated or exposed to low-level chronic contamination, and not exposed, or exposed to single or double pulses of two environmental concentrations (7 and 14 microg L(-1)) of diuron. Exposure to pollution and its impact on biofilms were assessed by measuring pesticide concentrations in biofilms, biomass parameters (chl a, AFDW), community structure (using 18S and 16S rDNA gene analysis by DGGE, and HPLC pigment analysis to target eukaryotes, bacteria and photoautotrophs, respectively) and by performing a physiological test. Control biofilms displayed very low diuron concentrations, whereas the herbicide was found in the contaminated biofilms. Nevertheless, diuron concentrations were not higher in the pulsed biofilms than in the non-pulsed ones. AFDW and chl ain vivo fluorescence increased in both microcosms during the experiment and biomass was higher in chronically exposed biofilms than in control ones. The impact on biomass was higher for the control double-pulsed biofilms than for the non-pulsed ones. Carbon incorporation by the chronically exposed biofilms was greater during the first 28 days of growth than during the first 28 days of growth in the control biofilms. Both single and double pulses inhibited carbon incorporation of all biofilm communities, especially of the control ones. Short-term inhibition of photosynthesis was never significantly different in exposed and non-exposed biofilms. Few differences in the pigment structure were found between chronically exposed and control biofilms, but pulses impacted on the pigment structure of all biofilm communities. Bacterial structural differences were observed between single-pulsed and non-pulsed biofilms, but not between double-pulsed and non-pulsed biofilms. The different pulses affected the eukaryotic community structures of the control biofilms, but not of the chronically exposed ones. Unlike the bacterial communities, the control eukaryotic communities were structurally different from the chronically exposed ones. This preliminary experimental study indicates that exposure to environmental concentrations of diuron and other agricultural contaminants and further exposure to diuron can have measurable effects on small river biofilm communities. The effects of a pulsed acute exposure to diuron on biofilms depended on whether the biofilms had previously been exposed to the same stressors or not. SN - 0166-445X UR - https://www.unboundmedicine.com/medline/citation/18387680/Responses_of_chronically_contaminated_biofilms_to_short_pulses_of_diuron__An_experimental_study_simulating_flooding_events_in_a_small_river_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0166-445X(08)00063-5 DB - PRIME DP - Unbound Medicine ER -