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Liquid chromatography tandem mass spectrometry assay for the simultaneous determination of venlafaxine and O-desmethylvenlafaxine in human plasma and its application to a bioequivalence study.
J Pharm Biomed Anal. 2008 Jul 15; 47(3):603-11.JP

Abstract

A rapid, simple and sensitive liquid chromatography tandem mass spectrometry (LC-MS/MS) assay for simultaneous determination of venlafaxine (VEN) and its active metabolite, O-desmethylvenlafaxine (ODV) in human plasma was developed using nadolol as internal standard (IS). The analytes and IS were extracted from 200 microl aliquots of human plasma via protein precipitation using 0.43% formic acid in acetonitrile and separated on a Hypurity cyano (50 mm x 4.6 mm, 5 microm) column. Quantitation was performed on a triple quadrupole mass spectrometer employing electrospray ionization technique, operating in multiple reaction monitoring (MRM) and positive ion mode. The precursor to product ion transitions monitored for VEN, ODV and IS were m/z 278.3-->58.1, 264.3-->58.1 and 310.4-->254.1, respectively. The total chromatographic runtime was 3 min with retention time for VEN, ODV and IS at 1.93, 1.50 and 1.29 min, respectively. The method was fully validated for its sensitivity, accuracy and precision, linearity, recovery, matrix effect, dilution integrity and stability studies. The linear dynamic range of 2.0-500 ng/ml was established for both VEN and ODV with mean correlation coefficient (r), 0.9994 and 0.9990, respectively. The intra-batch and inter-batch precision (%CV) in three validation batches across five concentration levels (LLOQ, LQC, MQC, HQC and ULOQ) was less than 12.6% for both the analytes. The accuracy determined at these levels was within -9.8 to +3.9% in terms of %bias. The method was successfully applied to a bioequivalence study of 150 mg venlafaxine extended release capsule formulation in 22 healthy Indian male subjects under fed condition.

Authors+Show Affiliations

Chemistry Department, School of Sciences, Gujarat University, Navrangpura, Ahmedabad 380009, Gujarat, India.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

18387768

Citation

Patel, Bhavin N., et al. "Liquid Chromatography Tandem Mass Spectrometry Assay for the Simultaneous Determination of Venlafaxine and O-desmethylvenlafaxine in Human Plasma and Its Application to a Bioequivalence Study." Journal of Pharmaceutical and Biomedical Analysis, vol. 47, no. 3, 2008, pp. 603-11.
Patel BN, Sharma N, Sanyal M, et al. Liquid chromatography tandem mass spectrometry assay for the simultaneous determination of venlafaxine and O-desmethylvenlafaxine in human plasma and its application to a bioequivalence study. J Pharm Biomed Anal. 2008;47(3):603-11.
Patel, B. N., Sharma, N., Sanyal, M., & Shrivastav, P. S. (2008). Liquid chromatography tandem mass spectrometry assay for the simultaneous determination of venlafaxine and O-desmethylvenlafaxine in human plasma and its application to a bioequivalence study. Journal of Pharmaceutical and Biomedical Analysis, 47(3), 603-11. https://doi.org/10.1016/j.jpba.2008.02.015
Patel BN, et al. Liquid Chromatography Tandem Mass Spectrometry Assay for the Simultaneous Determination of Venlafaxine and O-desmethylvenlafaxine in Human Plasma and Its Application to a Bioequivalence Study. J Pharm Biomed Anal. 2008 Jul 15;47(3):603-11. PubMed PMID: 18387768.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Liquid chromatography tandem mass spectrometry assay for the simultaneous determination of venlafaxine and O-desmethylvenlafaxine in human plasma and its application to a bioequivalence study. AU - Patel,Bhavin N, AU - Sharma,Naveen, AU - Sanyal,Mallika, AU - Shrivastav,Pranav S, Y1 - 2008/02/26/ PY - 2007/11/21/received PY - 2008/01/22/revised PY - 2008/02/18/accepted PY - 2008/4/5/pubmed PY - 2008/9/10/medline PY - 2008/4/5/entrez SP - 603 EP - 11 JF - Journal of pharmaceutical and biomedical analysis JO - J Pharm Biomed Anal VL - 47 IS - 3 N2 - A rapid, simple and sensitive liquid chromatography tandem mass spectrometry (LC-MS/MS) assay for simultaneous determination of venlafaxine (VEN) and its active metabolite, O-desmethylvenlafaxine (ODV) in human plasma was developed using nadolol as internal standard (IS). The analytes and IS were extracted from 200 microl aliquots of human plasma via protein precipitation using 0.43% formic acid in acetonitrile and separated on a Hypurity cyano (50 mm x 4.6 mm, 5 microm) column. Quantitation was performed on a triple quadrupole mass spectrometer employing electrospray ionization technique, operating in multiple reaction monitoring (MRM) and positive ion mode. The precursor to product ion transitions monitored for VEN, ODV and IS were m/z 278.3-->58.1, 264.3-->58.1 and 310.4-->254.1, respectively. The total chromatographic runtime was 3 min with retention time for VEN, ODV and IS at 1.93, 1.50 and 1.29 min, respectively. The method was fully validated for its sensitivity, accuracy and precision, linearity, recovery, matrix effect, dilution integrity and stability studies. The linear dynamic range of 2.0-500 ng/ml was established for both VEN and ODV with mean correlation coefficient (r), 0.9994 and 0.9990, respectively. The intra-batch and inter-batch precision (%CV) in three validation batches across five concentration levels (LLOQ, LQC, MQC, HQC and ULOQ) was less than 12.6% for both the analytes. The accuracy determined at these levels was within -9.8 to +3.9% in terms of %bias. The method was successfully applied to a bioequivalence study of 150 mg venlafaxine extended release capsule formulation in 22 healthy Indian male subjects under fed condition. SN - 0731-7085 UR - https://www.unboundmedicine.com/medline/citation/18387768/Liquid_chromatography_tandem_mass_spectrometry_assay_for_the_simultaneous_determination_of_venlafaxine_and_O_desmethylvenlafaxine_in_human_plasma_and_its_application_to_a_bioequivalence_study_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0731-7085(08)00113-1 DB - PRIME DP - Unbound Medicine ER -