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CD4+ CD25+ Treg cells inhibit human memory gammadelta T cells to produce IFN-gamma in response to M tuberculosis antigen ESAT-6.
Blood. 2008 Jun 15; 111(12):5629-36.Blood

Abstract

Gammadelta T cells play an important role in innate immunity against infections; however, the regulation of these cells remains largely unknown. In the present study, we show that ESAT-6, an antigen of Mycobacterium tuberculosis, induces IFN-gamma secretion by human gammadelta T cells. In addition, ESAT-6 also induces the activation and proliferation of gammadelta T cells. Phenotypic analysis indicates that IFN-gamma-producing gammadelta T cells are mainly effector memory cells with the surface phenotype of CD45RA(-)CD62L(-)CCR7(-). These results were further confirmed by the fact that naive gammadelta T cells from cord blood did not produce IFN-gamma in response to ESAT-6. Further studies indicated that stimulation with ESAT-6 directly induced purified gammadelta T cells to produce IFN-gamma, independent of both antigen-presenting cells and CD4(+) T cells. Unexpectedly, depletion of CD4(+) T cells markedly enhanced IFN-gamma production by gammadelta T cells, indicating that CD4(+) T cells regulate the response of gammadelta T cells. Importantly, CD4(+)CD25(+) T regulatory (Treg) cells but not CD4(+)CD25(-) T cells significantly inhibited IFN-gamma production by gammadelta T cells. Taken together, these data demonstrate for the first time that Treg cells can play an important role in the regulation of immune responses of antigen-specific human memory gammadelta T cells.

Authors+Show Affiliations

Department of Immunology, Zhongshan School of Medicine, Key Laboratory of Tropical Disease Control Research of Ministry of Education, Sun Yat-Sen University, Guangzhou, China.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18388182

Citation

Li, Li, and Chang-You Wu. "CD4+ CD25+ Treg Cells Inhibit Human Memory Gammadelta T Cells to Produce IFN-gamma in Response to M Tuberculosis Antigen ESAT-6." Blood, vol. 111, no. 12, 2008, pp. 5629-36.
Li L, Wu CY. CD4+ CD25+ Treg cells inhibit human memory gammadelta T cells to produce IFN-gamma in response to M tuberculosis antigen ESAT-6. Blood. 2008;111(12):5629-36.
Li, L., & Wu, C. Y. (2008). CD4+ CD25+ Treg cells inhibit human memory gammadelta T cells to produce IFN-gamma in response to M tuberculosis antigen ESAT-6. Blood, 111(12), 5629-36. https://doi.org/10.1182/blood-2008-02-139899
Li L, Wu CY. CD4+ CD25+ Treg Cells Inhibit Human Memory Gammadelta T Cells to Produce IFN-gamma in Response to M Tuberculosis Antigen ESAT-6. Blood. 2008 Jun 15;111(12):5629-36. PubMed PMID: 18388182.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - CD4+ CD25+ Treg cells inhibit human memory gammadelta T cells to produce IFN-gamma in response to M tuberculosis antigen ESAT-6. AU - Li,Li, AU - Wu,Chang-You, Y1 - 2008/04/03/ PY - 2008/4/5/pubmed PY - 2008/7/17/medline PY - 2008/4/5/entrez SP - 5629 EP - 36 JF - Blood JO - Blood VL - 111 IS - 12 N2 - Gammadelta T cells play an important role in innate immunity against infections; however, the regulation of these cells remains largely unknown. In the present study, we show that ESAT-6, an antigen of Mycobacterium tuberculosis, induces IFN-gamma secretion by human gammadelta T cells. In addition, ESAT-6 also induces the activation and proliferation of gammadelta T cells. Phenotypic analysis indicates that IFN-gamma-producing gammadelta T cells are mainly effector memory cells with the surface phenotype of CD45RA(-)CD62L(-)CCR7(-). These results were further confirmed by the fact that naive gammadelta T cells from cord blood did not produce IFN-gamma in response to ESAT-6. Further studies indicated that stimulation with ESAT-6 directly induced purified gammadelta T cells to produce IFN-gamma, independent of both antigen-presenting cells and CD4(+) T cells. Unexpectedly, depletion of CD4(+) T cells markedly enhanced IFN-gamma production by gammadelta T cells, indicating that CD4(+) T cells regulate the response of gammadelta T cells. Importantly, CD4(+)CD25(+) T regulatory (Treg) cells but not CD4(+)CD25(-) T cells significantly inhibited IFN-gamma production by gammadelta T cells. Taken together, these data demonstrate for the first time that Treg cells can play an important role in the regulation of immune responses of antigen-specific human memory gammadelta T cells. SN - 1528-0020 UR - https://www.unboundmedicine.com/medline/citation/18388182/CD4+_CD25+_Treg_cells_inhibit_human_memory_gammadelta_T_cells_to_produce_IFN_gamma_in_response_to_M_tuberculosis_antigen_ESAT_6_ L2 - https://ashpublications.org/blood/article-lookup/doi/10.1182/blood-2008-02-139899 DB - PRIME DP - Unbound Medicine ER -