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Distribution of Streptococcus mutans and Streptococcus sobrinus in saliva of Mexican preschool caries-free and caries-active children by microbial and molecular (PCR) assays.
J Clin Pediatr Dent. 2008 Winter; 32(2):121-6.JC

Abstract

The aim of the study was to investigate the distribution of mutans streptococci (MS) infection of caries-free (CF) and caries-active (CA) preschool Mexican children by microbial and molecular assays. Eighty preschool children were divided into two groups, 40 CF and 40 CA children. Saliva samples were inoculated onto MSB to identify CFU and DNA extractions were tested by PCR. Our results indicated that there was no statistical difference (p > 0.05) between groups either in age, weight, height or sex. S. sobrinus was detected by PCR twice as much in the CA group, the difference being statistically significant (p < 0.05). dmfs index was positive correlated with S. mutans (r = 0.2941, p = 0.0081), S. sobrinus (r = 0.3384, p = 0.0021) and S. mutans-S. sobrinus (r = 0.3978, p = 0.0003). ANCOVA revealed that dmfs index had a significant effect on the distribution of CFU of S. mutans (p = 0.0118) and S. sobrinus (p = 0.03). When MSB was compared with PCR to identify MS, there was no statistical difference (p > 0.05). We conclude that S. mutans and S. sobrinus were isolated in higher numbers from CA children and those harbouring both bacteria had higher dmfs scores. PCR is a useful tool in molecular epidemiology for dental caries studies; it was effective in detecting and identifying MS from saliva in children.

Authors+Show Affiliations

San Luis Potosi University, Mexico. jloyola@uaslp.mxNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18389677

Citation

Loyola-Rodriguez, Juan Pablo, et al. "Distribution of Streptococcus Mutans and Streptococcus Sobrinus in Saliva of Mexican Preschool Caries-free and Caries-active Children By Microbial and Molecular (PCR) Assays." The Journal of Clinical Pediatric Dentistry, vol. 32, no. 2, 2008, pp. 121-6.
Loyola-Rodriguez JP, Martinez-Martinez RE, Flores-Ferreyra BI, et al. Distribution of Streptococcus mutans and Streptococcus sobrinus in saliva of Mexican preschool caries-free and caries-active children by microbial and molecular (PCR) assays. J Clin Pediatr Dent. 2008;32(2):121-6.
Loyola-Rodriguez, J. P., Martinez-Martinez, R. E., Flores-Ferreyra, B. I., Patiño-Marin, N., Alpuche-Solis, A. G., & Reyes-Macias, J. F. (2008). Distribution of Streptococcus mutans and Streptococcus sobrinus in saliva of Mexican preschool caries-free and caries-active children by microbial and molecular (PCR) assays. The Journal of Clinical Pediatric Dentistry, 32(2), 121-6.
Loyola-Rodriguez JP, et al. Distribution of Streptococcus Mutans and Streptococcus Sobrinus in Saliva of Mexican Preschool Caries-free and Caries-active Children By Microbial and Molecular (PCR) Assays. J Clin Pediatr Dent. 2008;32(2):121-6. PubMed PMID: 18389677.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Distribution of Streptococcus mutans and Streptococcus sobrinus in saliva of Mexican preschool caries-free and caries-active children by microbial and molecular (PCR) assays. AU - Loyola-Rodriguez,Juan Pablo, AU - Martinez-Martinez,Rita Elizabeth, AU - Flores-Ferreyra,Blanca Irma, AU - Patiño-Marin,Nuria, AU - Alpuche-Solis,Angel Gabriel, AU - Reyes-Macias,Juan Francisco, PY - 2008/4/9/pubmed PY - 2008/5/23/medline PY - 2008/4/9/entrez SP - 121 EP - 6 JF - The Journal of clinical pediatric dentistry JO - J Clin Pediatr Dent VL - 32 IS - 2 N2 - The aim of the study was to investigate the distribution of mutans streptococci (MS) infection of caries-free (CF) and caries-active (CA) preschool Mexican children by microbial and molecular assays. Eighty preschool children were divided into two groups, 40 CF and 40 CA children. Saliva samples were inoculated onto MSB to identify CFU and DNA extractions were tested by PCR. Our results indicated that there was no statistical difference (p > 0.05) between groups either in age, weight, height or sex. S. sobrinus was detected by PCR twice as much in the CA group, the difference being statistically significant (p < 0.05). dmfs index was positive correlated with S. mutans (r = 0.2941, p = 0.0081), S. sobrinus (r = 0.3384, p = 0.0021) and S. mutans-S. sobrinus (r = 0.3978, p = 0.0003). ANCOVA revealed that dmfs index had a significant effect on the distribution of CFU of S. mutans (p = 0.0118) and S. sobrinus (p = 0.03). When MSB was compared with PCR to identify MS, there was no statistical difference (p > 0.05). We conclude that S. mutans and S. sobrinus were isolated in higher numbers from CA children and those harbouring both bacteria had higher dmfs scores. PCR is a useful tool in molecular epidemiology for dental caries studies; it was effective in detecting and identifying MS from saliva in children. SN - 1053-4628 UR - https://www.unboundmedicine.com/medline/citation/18389677/Distribution_of_Streptococcus_mutans_and_Streptococcus_sobrinus_in_saliva_of_Mexican_preschool_caries_free_and_caries_active_children_by_microbial_and_molecular__PCR__assays_ DB - PRIME DP - Unbound Medicine ER -