TY - JOUR T1 - [Detecting nicotine and its metabolite cotinine in human hair with capillary gas chromatography]. AU - Gu,Su-ying, AU - Li,Yuan-qian, AU - Liu,Chi-qing, AU - Yang,Xiao-qi, AU - Li,Lei, AU - Ma,Shao-jun, AU - Yang,Gong-huan, PY - 2008/4/9/pubmed PY - 2010/5/21/medline PY - 2008/4/9/entrez SP - 133 EP - 6 JF - Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition JO - Sichuan Da Xue Xue Bao Yi Xue Ban VL - 39 IS - 1 N2 - OBJECTIVE: To develop a rapid method for determination of nicotine and its metabolite cotinine in human hair with capillary gas chromatography with flame ionization detector. METHODS: The hair sample was digested by 1.5 mol/L sodium hydroxide solution. The nicotine and cotinine in the hair sample were extracted with a mixed solvent of dichloromethane-methanol (3:1). Aliquot of the extraction solution was vaporized with nitrogen flow and then methanol was added to dissolve the analysts. The analysts were tested with capillary gas chromatography. RESULTS: The detection limits (signal to noise ratio of 3:1) were 4.3 ng/mL for nicotine and 10 ng/mL for cotinine, respectively. The spiked recoveries were 90.33%-113.1% for nicotine with relative standard deviation (RSD) of 2.1%-7.0% and 92.92%-117.4% for cotinine with relative standard deviation (RSD) of 4.4%-8.9%, respectively. CONCLUSION: The proposed method is sensitive, simple, easy and rapid. It can be easily extended to large scale applications in tobacco epidemiology studies. SN - 1672-173X UR - https://www.unboundmedicine.com/medline/citation/18390221/[Detecting_nicotine_and_its_metabolite_cotinine_in_human_hair_with_capillary_gas_chromatography]_ DB - PRIME DP - Unbound Medicine ER -