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Capillary electrophoresis with laser-induced fluorescence: environmental applications.
Methods Mol Biol. 2008; 384:119-34.MM

Abstract

Capillary electrophoresis (CE), especially free-zone CE, offers a relatively simple separation with moderate selectivity based on the mobility of ions in solution. Laser-induced fluorescence (LIF) detection, an extremely sensitive technique, can be coupled with a variety of separation conditions to achieve sensitive and quantitative results. When these techniques are combined, CE/LIF provides the sensitivity and increased selectivity that makes trace level environmental analysis of fluorescent compounds possible at or below levels typical for gas chromatography (GC)/mass spectrometry (MS). We offer a panoramic review of the role of these tools in solving environmental and related analytical problems before providing a detailed experimental protocol.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Riddick L
National Exposure Research Laboratory, Environmental Sciences Division, US Environmental Protection Agency, Office of Research and Development, Las Vegas, NV, USA.
Brumley WC
No affiliation info available

MeSH

Electrophoresis, CapillaryEnvironmentEnvironmental MonitoringFluoresceinsFluorescenceLasersSolid Phase ExtractionSpecimen Handling

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

18392568

Citation

Riddick, Lee, and William C. Brumley. "Capillary Electrophoresis With Laser-induced Fluorescence: Environmental Applications." Methods in Molecular Biology (Clifton, N.J.), vol. 384, 2008, pp. 119-34.
Riddick L, Brumley WC. Capillary electrophoresis with laser-induced fluorescence: environmental applications. Methods Mol Biol. 2008;384:119-34.
Riddick, L., & Brumley, W. C. (2008). Capillary electrophoresis with laser-induced fluorescence: environmental applications. Methods in Molecular Biology (Clifton, N.J.), 384, 119-34. https://doi.org/10.1007/978-1-59745-376-9_6
Riddick L, Brumley WC. Capillary Electrophoresis With Laser-induced Fluorescence: Environmental Applications. Methods Mol Biol. 2008;384:119-34. PubMed PMID: 18392568.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Capillary electrophoresis with laser-induced fluorescence: environmental applications. AU - Riddick,Lee, AU - Brumley,William C, PY - 2008/4/9/pubmed PY - 2008/5/8/medline PY - 2008/4/9/entrez SP - 119 EP - 34 JF - Methods in molecular biology (Clifton, N.J.) JO - Methods Mol Biol VL - 384 N2 - Capillary electrophoresis (CE), especially free-zone CE, offers a relatively simple separation with moderate selectivity based on the mobility of ions in solution. Laser-induced fluorescence (LIF) detection, an extremely sensitive technique, can be coupled with a variety of separation conditions to achieve sensitive and quantitative results. When these techniques are combined, CE/LIF provides the sensitivity and increased selectivity that makes trace level environmental analysis of fluorescent compounds possible at or below levels typical for gas chromatography (GC)/mass spectrometry (MS). We offer a panoramic review of the role of these tools in solving environmental and related analytical problems before providing a detailed experimental protocol. SN - 1064-3745 UR - https://www.unboundmedicine.com/medline/citation/18392568/Capillary_electrophoresis_with_laser_induced_fluorescence:_environmental_applications_ DB - PRIME DP - Unbound Medicine ER -