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Molecular mapping of the novel powdery mildew resistance gene Pm36 introgressed from Triticum turgidum var. dicoccoides in durum wheat.
Theor Appl Genet. 2008 Jun; 117(1):135-42.TA

Abstract

Powdery mildew, caused by Blumeria graminis f.sp. tritici, is one of the most important wheat diseases in many regions of the world. Triticum turgidum var. dicoccoides (2n=4x=AABB), the progenitor of cultivated wheats, shows particular promises as a donor of useful genetic variation for several traits, including disease resistances. The wild emmer accession MG29896, resistant to powdery mildew, was backcrossed to the susceptible durum wheat cultivar Latino, and a set of backcross inbred lines (BC(5)F(5)) was produced. Genetic analysis of F(3) populations from two resistant introgression lines (5BIL-29 x Latino and 5BIL-42 x Latino) indicated that the powdery mildew resistance is controlled by a single dominant gene. Molecular markers and the bulked segregant analysis were used to characterize and map the powdery mildew resistance. Five AFLP markers (XP43M32((250)), XP46M31((410)), XP41M37((100)), XP41M39((250)), XP39M32((120))), three genomic SSR markers (Xcfd07, Xwmc75, Xgwm408) and one EST-derived SSR marker (BJ261635) were found to be linked to the resistance gene in 5BIL-29 and only the BJ261635 marker in 5BIL-42. By means of Chinese Spring nullisomic-tetrasomic, ditelosomic and deletion lines, the polymorphic markers and the resistance gene were assigned to chromosome bin 5BL6-0.29-0.76. These results indicated that the two lines had the same resistance gene and that the introgressed dicoccoides chromosome segment was longer (35.5 cM) in 5BIL-29 than that introgressed in 5BIL-42 (less than 1.5 cM). As no powdery mildew resistance gene has been reported on chromosome arm 5BL, the novel resistance gene derived from var. dicoccoides was designated Pm36. The 244 bp allele of BJ261635 in 5BIL-42 can be used for marker-assisted selection during the wheat resistance breeding process for facilitating gene pyramiding.

Authors+Show Affiliations

Department of Agro-Forestry and Environmental Biology and Chemistry, University of Bari, via Amendola, 165/A, 70126 Bari, Italy. blanco@agr.uniba.itNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18392800

Citation

Blanco, Antonio, et al. "Molecular Mapping of the Novel Powdery Mildew Resistance Gene Pm36 Introgressed From Triticum Turgidum Var. Dicoccoides in Durum Wheat." TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, vol. 117, no. 1, 2008, pp. 135-42.
Blanco A, Gadaleta A, Cenci A, et al. Molecular mapping of the novel powdery mildew resistance gene Pm36 introgressed from Triticum turgidum var. dicoccoides in durum wheat. Theor Appl Genet. 2008;117(1):135-42.
Blanco, A., Gadaleta, A., Cenci, A., Carluccio, A. V., Abdelbacki, A. M., & Simeone, R. (2008). Molecular mapping of the novel powdery mildew resistance gene Pm36 introgressed from Triticum turgidum var. dicoccoides in durum wheat. TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, 117(1), 135-42. https://doi.org/10.1007/s00122-008-0760-0
Blanco A, et al. Molecular Mapping of the Novel Powdery Mildew Resistance Gene Pm36 Introgressed From Triticum Turgidum Var. Dicoccoides in Durum Wheat. Theor Appl Genet. 2008;117(1):135-42. PubMed PMID: 18392800.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular mapping of the novel powdery mildew resistance gene Pm36 introgressed from Triticum turgidum var. dicoccoides in durum wheat. AU - Blanco,Antonio, AU - Gadaleta,A, AU - Cenci,A, AU - Carluccio,A V, AU - Abdelbacki,A M M, AU - Simeone,R, Y1 - 2008/04/08/ PY - 2007/11/12/received PY - 2008/03/26/accepted PY - 2008/4/9/pubmed PY - 2008/8/30/medline PY - 2008/4/9/entrez SP - 135 EP - 42 JF - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JO - Theor Appl Genet VL - 117 IS - 1 N2 - Powdery mildew, caused by Blumeria graminis f.sp. tritici, is one of the most important wheat diseases in many regions of the world. Triticum turgidum var. dicoccoides (2n=4x=AABB), the progenitor of cultivated wheats, shows particular promises as a donor of useful genetic variation for several traits, including disease resistances. The wild emmer accession MG29896, resistant to powdery mildew, was backcrossed to the susceptible durum wheat cultivar Latino, and a set of backcross inbred lines (BC(5)F(5)) was produced. Genetic analysis of F(3) populations from two resistant introgression lines (5BIL-29 x Latino and 5BIL-42 x Latino) indicated that the powdery mildew resistance is controlled by a single dominant gene. Molecular markers and the bulked segregant analysis were used to characterize and map the powdery mildew resistance. Five AFLP markers (XP43M32((250)), XP46M31((410)), XP41M37((100)), XP41M39((250)), XP39M32((120))), three genomic SSR markers (Xcfd07, Xwmc75, Xgwm408) and one EST-derived SSR marker (BJ261635) were found to be linked to the resistance gene in 5BIL-29 and only the BJ261635 marker in 5BIL-42. By means of Chinese Spring nullisomic-tetrasomic, ditelosomic and deletion lines, the polymorphic markers and the resistance gene were assigned to chromosome bin 5BL6-0.29-0.76. These results indicated that the two lines had the same resistance gene and that the introgressed dicoccoides chromosome segment was longer (35.5 cM) in 5BIL-29 than that introgressed in 5BIL-42 (less than 1.5 cM). As no powdery mildew resistance gene has been reported on chromosome arm 5BL, the novel resistance gene derived from var. dicoccoides was designated Pm36. The 244 bp allele of BJ261635 in 5BIL-42 can be used for marker-assisted selection during the wheat resistance breeding process for facilitating gene pyramiding. SN - 0040-5752 UR - https://www.unboundmedicine.com/medline/citation/18392800/Molecular_mapping_of_the_novel_powdery_mildew_resistance_gene_Pm36_introgressed_from_Triticum_turgidum_var__dicoccoides_in_durum_wheat_ L2 - https://dx.doi.org/10.1007/s00122-008-0760-0 DB - PRIME DP - Unbound Medicine ER -