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Proteins and peptides of the salivary gland secretion of medicinal leeches Hirudo verbana, H. medicinalis, and H. orientalis.
Biochemistry (Mosc). 2008 Mar; 73(3):315-20.B

Abstract

The protein and peptide composition of medicinal leech salivary gland secretion (SGS) was analyzed in preparations obtained in July from three species--Hirudo verbana, H. medicinalis, and H. orientalis. Two-dimensional electrophoresis (molecular mass 10-150 kD and pI 3-10) revealed no distinctions in the distribution of over 100 silver-stained proteins. Differences were noted only in intensity of 10 protein spots at 30-90 kD and pI 4.7-7.5. Mass spectrometric profiling of SGS of the three leech species using the Zip-Tip/golden chip scheme and cation-exchanging chips CM-10 revealed over 50 components in SGS of each of the three leech species. It was noted that 30-40% of the individual masses of the SGS of each leech species fall within the masses present in SGS of at least one of the two other species. This rather small part of the total mass may be indicative of a high polymorphism of amino acid sequences or a high frequency of posttranslational modifications of the SGS proteins and peptides. Calculation of Jacquard's coefficient showed that H. medicinalis and H. orientalis are closest to each other in SGS composition, which is consistent with data in the literature on the phylogenetic relationship between these two species of medicinal leech. Comparison of detected molecular masses with those of six known biologically active compounds produced by medicinal leeches revealed their uneven distribution in SGS of each of the three medicinal leech species. This opens prospects for using certain species of medicinal leech for targeted therapy of various pathologies.

Authors+Show Affiliations

Biological Faculty, Lomonosov Moscow State University, Moscow, Russia. saliva1@yandex.ruNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18393768

Citation

Baskova, I P., et al. "Proteins and Peptides of the Salivary Gland Secretion of Medicinal Leeches Hirudo Verbana, H. Medicinalis, and H. Orientalis." Biochemistry. Biokhimiia, vol. 73, no. 3, 2008, pp. 315-20.
Baskova IP, Kostrjukova ES, Vlasova MA, et al. Proteins and peptides of the salivary gland secretion of medicinal leeches Hirudo verbana, H. medicinalis, and H. orientalis. Biochemistry Mosc. 2008;73(3):315-20.
Baskova, I. P., Kostrjukova, E. S., Vlasova, M. A., Kharitonova, O. V., Levitskiy, S. A., Zavalova, L. L., Moshkovskii, S. A., & Lazarev, V. N. (2008). Proteins and peptides of the salivary gland secretion of medicinal leeches Hirudo verbana, H. medicinalis, and H. orientalis. Biochemistry. Biokhimiia, 73(3), 315-20.
Baskova IP, et al. Proteins and Peptides of the Salivary Gland Secretion of Medicinal Leeches Hirudo Verbana, H. Medicinalis, and H. Orientalis. Biochemistry Mosc. 2008;73(3):315-20. PubMed PMID: 18393768.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Proteins and peptides of the salivary gland secretion of medicinal leeches Hirudo verbana, H. medicinalis, and H. orientalis. AU - Baskova,I P, AU - Kostrjukova,E S, AU - Vlasova,M A, AU - Kharitonova,O V, AU - Levitskiy,S A, AU - Zavalova,L L, AU - Moshkovskii,S A, AU - Lazarev,V N, PY - 2008/4/9/pubmed PY - 2008/5/28/medline PY - 2008/4/9/entrez SP - 315 EP - 20 JF - Biochemistry. Biokhimiia JO - Biochemistry Mosc. VL - 73 IS - 3 N2 - The protein and peptide composition of medicinal leech salivary gland secretion (SGS) was analyzed in preparations obtained in July from three species--Hirudo verbana, H. medicinalis, and H. orientalis. Two-dimensional electrophoresis (molecular mass 10-150 kD and pI 3-10) revealed no distinctions in the distribution of over 100 silver-stained proteins. Differences were noted only in intensity of 10 protein spots at 30-90 kD and pI 4.7-7.5. Mass spectrometric profiling of SGS of the three leech species using the Zip-Tip/golden chip scheme and cation-exchanging chips CM-10 revealed over 50 components in SGS of each of the three leech species. It was noted that 30-40% of the individual masses of the SGS of each leech species fall within the masses present in SGS of at least one of the two other species. This rather small part of the total mass may be indicative of a high polymorphism of amino acid sequences or a high frequency of posttranslational modifications of the SGS proteins and peptides. Calculation of Jacquard's coefficient showed that H. medicinalis and H. orientalis are closest to each other in SGS composition, which is consistent with data in the literature on the phylogenetic relationship between these two species of medicinal leech. Comparison of detected molecular masses with those of six known biologically active compounds produced by medicinal leeches revealed their uneven distribution in SGS of each of the three medicinal leech species. This opens prospects for using certain species of medicinal leech for targeted therapy of various pathologies. SN - 0006-2979 UR - https://www.unboundmedicine.com/medline/citation/18393768/Proteins_and_peptides_of_the_salivary_gland_secretion_of_medicinal_leeches_Hirudo_verbana_H__medicinalis_and_H__orientalis_ L2 - http://protein.bio.msu.ru/biokhimiya/contents/v73/full/73030388.html DB - PRIME DP - Unbound Medicine ER -