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Retinal ganglion cell neuroprotection in a rat model of glaucoma following brimonidine, latanoprost or combined treatments.
Exp Eye Res. 2008 May; 86(5):798-806.EE

Abstract

The aim of the present study is to evaluate the neuroprotective effect of two antiglaucomatous substances, regardless of their hypotensive effect in the eye. Brimonidine, which does not reduce IOP when administered intraperitoneally, and latanoprost, which has a renowned hypotensive effect topically. We examined rat retinal ganglion cell (RGC) survival and size distribution in experimental glaucoma in response to different glaucomatous agents. IOP was elevated by episcleral vein cauterization (EVC) prior to the application of different treatments: (I) PBS application (control group), (II) intraperitoneal administration of brimonidine (a general hypotensive agent), (III) topical application of latanoprost (an ocular hypotensive agent), and (IV) latanoprost combined with brimonidine. After 12 weeks, RGCs were retrogradely labeled with fluorogold and RGC density was analyzed. EVC caused a significant increase (42%) in IOP in each group before drug treatment. After 12weeks of EVC, RGC survival in control vs. EVC rats was 78.9+/-3.2%. No IOP reduction was observed in brimonidine injected rats, but RGC survival at 12 weeks was total (103.7+/-2.7%). In latanoprost treated rats, IOP dropped by around 22% and 94.7+/-3.7% of the RGC population survived. Finally in the latanoprost+brimonidine combined group, IOP was significantly reduced by 25% and 94.4+/-2.2% of RGCs survived. Surprisingly, whereas EVC led to a 6% increase in RGC soma size, brimonidine treatment was associated with a 9% reduction in the soma size of RGCs at 12 weeks. We conclude that brimonidine exerts a neuroprotective effect via a mechanism which is independent of IOP reduction. These findings indicate that cell survival in glaucoma may be enhanced by neuroprotective strategies which are independent of IOP reduction. No synergistic neuroprotective effect was observed when both treatments were applied simultaneously.

Authors+Show Affiliations

Department of Cell Biology and Histology, University of the Basque Country, Leioa, Vizcaya, Spain.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18394603

Citation

Hernández, María, et al. "Retinal Ganglion Cell Neuroprotection in a Rat Model of Glaucoma Following Brimonidine, Latanoprost or Combined Treatments." Experimental Eye Research, vol. 86, no. 5, 2008, pp. 798-806.
Hernández M, Urcola JH, Vecino E. Retinal ganglion cell neuroprotection in a rat model of glaucoma following brimonidine, latanoprost or combined treatments. Exp Eye Res. 2008;86(5):798-806.
Hernández, M., Urcola, J. H., & Vecino, E. (2008). Retinal ganglion cell neuroprotection in a rat model of glaucoma following brimonidine, latanoprost or combined treatments. Experimental Eye Research, 86(5), 798-806. https://doi.org/10.1016/j.exer.2008.02.008
Hernández M, Urcola JH, Vecino E. Retinal Ganglion Cell Neuroprotection in a Rat Model of Glaucoma Following Brimonidine, Latanoprost or Combined Treatments. Exp Eye Res. 2008;86(5):798-806. PubMed PMID: 18394603.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Retinal ganglion cell neuroprotection in a rat model of glaucoma following brimonidine, latanoprost or combined treatments. AU - Hernández,María, AU - Urcola,J Haritz, AU - Vecino,Elena, Y1 - 2008/03/04/ PY - 2007/07/04/received PY - 2008/02/18/revised PY - 2008/02/19/accepted PY - 2008/4/9/pubmed PY - 2008/9/16/medline PY - 2008/4/9/entrez SP - 798 EP - 806 JF - Experimental eye research JO - Exp Eye Res VL - 86 IS - 5 N2 - The aim of the present study is to evaluate the neuroprotective effect of two antiglaucomatous substances, regardless of their hypotensive effect in the eye. Brimonidine, which does not reduce IOP when administered intraperitoneally, and latanoprost, which has a renowned hypotensive effect topically. We examined rat retinal ganglion cell (RGC) survival and size distribution in experimental glaucoma in response to different glaucomatous agents. IOP was elevated by episcleral vein cauterization (EVC) prior to the application of different treatments: (I) PBS application (control group), (II) intraperitoneal administration of brimonidine (a general hypotensive agent), (III) topical application of latanoprost (an ocular hypotensive agent), and (IV) latanoprost combined with brimonidine. After 12 weeks, RGCs were retrogradely labeled with fluorogold and RGC density was analyzed. EVC caused a significant increase (42%) in IOP in each group before drug treatment. After 12weeks of EVC, RGC survival in control vs. EVC rats was 78.9+/-3.2%. No IOP reduction was observed in brimonidine injected rats, but RGC survival at 12 weeks was total (103.7+/-2.7%). In latanoprost treated rats, IOP dropped by around 22% and 94.7+/-3.7% of the RGC population survived. Finally in the latanoprost+brimonidine combined group, IOP was significantly reduced by 25% and 94.4+/-2.2% of RGCs survived. Surprisingly, whereas EVC led to a 6% increase in RGC soma size, brimonidine treatment was associated with a 9% reduction in the soma size of RGCs at 12 weeks. We conclude that brimonidine exerts a neuroprotective effect via a mechanism which is independent of IOP reduction. These findings indicate that cell survival in glaucoma may be enhanced by neuroprotective strategies which are independent of IOP reduction. No synergistic neuroprotective effect was observed when both treatments were applied simultaneously. SN - 0014-4835 UR - https://www.unboundmedicine.com/medline/citation/18394603/Retinal_ganglion_cell_neuroprotection_in_a_rat_model_of_glaucoma_following_brimonidine_latanoprost_or_combined_treatments_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0014-4835(08)00067-5 DB - PRIME DP - Unbound Medicine ER -