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A sandwich structured SiO(2)/cytochrome c/SiO(2) on a boron-doped diamond film electrode as an electrochemical nitrite biosensor.
Biomaterials. 2008 Jun; 29(18):2794-801.B

Abstract

A novel sandwich structured SiO(2) gel/cytochrome c (Cyt c)/SiO(2) gel was designed and constructed on conductive boron-doped diamond (BDD) film substrate. A SiO(2) gel membrane was first in situ deposited on the pretreated positive charged H-terminated BDD electrode with a simple and artful surface vapor sol-gel method. Cyt c was subsequently immobilized onto the SiO(2) membranes by electrostatic attraction, followed by another SiO(2) gel layer in situ depositing on it. The SiO(2) interlayer was conceived to play an important role in the resultant sandwich structured SiO(2)/Cyt c/SiO(2)/BDD electrode as a selective "semi-open" medium, which guaranteed the immobilized Cyt c to maintain high stability and perform good electrochemistry and biocatalysis responses. The bioactivity of Cyt c was well protected and the immobilized biomolecule even didn't denature at extremely high or low pH condition. More attractively, Cyt c in the sandwich structured electrode could be further oxidized into highly reactive Cyt c pi-cation by two-step electrochemical oxidation, which could oxidize NO(2)(-) into NO(3)(-) in the solution. A sensitive determination approach of nitrite was accordingly built up based on this biocatalytic oxidative interaction for the first time and a possible mechanism of the interaction was herein proposed.

Authors+Show Affiliations

Department of Chemistry, Tongji University, 1239 Siping Road, Shanghai 200092, People's Republic of China.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18394698

Citation

Geng, Rong, et al. "A Sandwich Structured SiO(2)/cytochrome c/SiO(2) On a Boron-doped Diamond Film Electrode as an Electrochemical Nitrite Biosensor." Biomaterials, vol. 29, no. 18, 2008, pp. 2794-801.
Geng R, Zhao G, Liu M, et al. A sandwich structured SiO(2)/cytochrome c/SiO(2) on a boron-doped diamond film electrode as an electrochemical nitrite biosensor. Biomaterials. 2008;29(18):2794-801.
Geng, R., Zhao, G., Liu, M., & Li, M. (2008). A sandwich structured SiO(2)/cytochrome c/SiO(2) on a boron-doped diamond film electrode as an electrochemical nitrite biosensor. Biomaterials, 29(18), 2794-801. https://doi.org/10.1016/j.biomaterials.2008.03.004
Geng R, et al. A Sandwich Structured SiO(2)/cytochrome c/SiO(2) On a Boron-doped Diamond Film Electrode as an Electrochemical Nitrite Biosensor. Biomaterials. 2008;29(18):2794-801. PubMed PMID: 18394698.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A sandwich structured SiO(2)/cytochrome c/SiO(2) on a boron-doped diamond film electrode as an electrochemical nitrite biosensor. AU - Geng,Rong, AU - Zhao,Guohua, AU - Liu,Meichuan, AU - Li,Mingfang, Y1 - 2008/04/03/ PY - 2008/01/16/received PY - 2008/03/04/accepted PY - 2008/4/9/pubmed PY - 2008/9/23/medline PY - 2008/4/9/entrez SP - 2794 EP - 801 JF - Biomaterials JO - Biomaterials VL - 29 IS - 18 N2 - A novel sandwich structured SiO(2) gel/cytochrome c (Cyt c)/SiO(2) gel was designed and constructed on conductive boron-doped diamond (BDD) film substrate. A SiO(2) gel membrane was first in situ deposited on the pretreated positive charged H-terminated BDD electrode with a simple and artful surface vapor sol-gel method. Cyt c was subsequently immobilized onto the SiO(2) membranes by electrostatic attraction, followed by another SiO(2) gel layer in situ depositing on it. The SiO(2) interlayer was conceived to play an important role in the resultant sandwich structured SiO(2)/Cyt c/SiO(2)/BDD electrode as a selective "semi-open" medium, which guaranteed the immobilized Cyt c to maintain high stability and perform good electrochemistry and biocatalysis responses. The bioactivity of Cyt c was well protected and the immobilized biomolecule even didn't denature at extremely high or low pH condition. More attractively, Cyt c in the sandwich structured electrode could be further oxidized into highly reactive Cyt c pi-cation by two-step electrochemical oxidation, which could oxidize NO(2)(-) into NO(3)(-) in the solution. A sensitive determination approach of nitrite was accordingly built up based on this biocatalytic oxidative interaction for the first time and a possible mechanism of the interaction was herein proposed. SN - 0142-9612 UR - https://www.unboundmedicine.com/medline/citation/18394698/A_sandwich_structured_SiO_2_/cytochrome_c/SiO_2__on_a_boron_doped_diamond_film_electrode_as_an_electrochemical_nitrite_biosensor_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0142-9612(08)00159-2 DB - PRIME DP - Unbound Medicine ER -