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Reverse micellar microextraction for rapid analysis of thiol-containing peptides and amino acids by atmospheric-pressure matrix-assisted laser desorption/ionization ion trap and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.
Rapid Commun Mass Spectrom. 2008 May; 22(9):1437-44.RC

Abstract

Simple, rapid and inexpensive one-step reverse micellar microextraction (RMME) procedures were combined with matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) for the determination of thiol-containing peptides and amino acids. In this investigation, a thiol-containing peptide (HW6) was chosen as model compound to understand the mechanism of RMME. The electrostatic interactions between the thiol-containing peptide and reverse micelles were proposed to be reason for the transfer of analytes from the aqueous phase to the organic phase. Reverse micelles were formed by the cationic surfactant, methyltrioctylammonium chloride (MTOAC). The best extraction efficiency of HW6 was obtained under the following conditions: pH 11.0, ionic strength 5.0 mM of KCl and micelle concentration 7.0 mM of MTOAC. The limits of detection (LODs) obtained for HW6 in water, urine and plasma samples were 0.15, 0.19 and 0.28 microM, respectively, with relative standard deviation (RSD) values in the range +/-8.8-10.5%. The sensitivity obtained in water by the present method was 45-fold higher than that of the conventional use of atmospheric-pressure (AP)-MALDI MS. Furthermore, the applicability of the proposed approach was extended for the determination of thiol-containing amino acids in sample solutions by using MALDI time-of-flight (TOF) MS.

Authors+Show Affiliations

Department of Chemistry, National Sun Yat-Sen University, Kaohsiung, 804, Taiwan.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18395891

Citation

Agrawal, Kavita, et al. "Reverse Micellar Microextraction for Rapid Analysis of Thiol-containing Peptides and Amino Acids By Atmospheric-pressure Matrix-assisted Laser Desorption/ionization Ion Trap and Matrix-assisted Laser Desorption/ionization Time-of-flight Mass Spectrometry." Rapid Communications in Mass Spectrometry : RCM, vol. 22, no. 9, 2008, pp. 1437-44.
Agrawal K, Wu HF, Shrivas K. Reverse micellar microextraction for rapid analysis of thiol-containing peptides and amino acids by atmospheric-pressure matrix-assisted laser desorption/ionization ion trap and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Rapid Commun Mass Spectrom. 2008;22(9):1437-44.
Agrawal, K., Wu, H. F., & Shrivas, K. (2008). Reverse micellar microextraction for rapid analysis of thiol-containing peptides and amino acids by atmospheric-pressure matrix-assisted laser desorption/ionization ion trap and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Rapid Communications in Mass Spectrometry : RCM, 22(9), 1437-44. https://doi.org/10.1002/rcm.3509
Agrawal K, Wu HF, Shrivas K. Reverse Micellar Microextraction for Rapid Analysis of Thiol-containing Peptides and Amino Acids By Atmospheric-pressure Matrix-assisted Laser Desorption/ionization Ion Trap and Matrix-assisted Laser Desorption/ionization Time-of-flight Mass Spectrometry. Rapid Commun Mass Spectrom. 2008;22(9):1437-44. PubMed PMID: 18395891.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Reverse micellar microextraction for rapid analysis of thiol-containing peptides and amino acids by atmospheric-pressure matrix-assisted laser desorption/ionization ion trap and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. AU - Agrawal,Kavita, AU - Wu,Hui-Fen, AU - Shrivas,Kamlesh, PY - 2008/4/9/pubmed PY - 2008/7/4/medline PY - 2008/4/9/entrez SP - 1437 EP - 44 JF - Rapid communications in mass spectrometry : RCM JO - Rapid Commun Mass Spectrom VL - 22 IS - 9 N2 - Simple, rapid and inexpensive one-step reverse micellar microextraction (RMME) procedures were combined with matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) for the determination of thiol-containing peptides and amino acids. In this investigation, a thiol-containing peptide (HW6) was chosen as model compound to understand the mechanism of RMME. The electrostatic interactions between the thiol-containing peptide and reverse micelles were proposed to be reason for the transfer of analytes from the aqueous phase to the organic phase. Reverse micelles were formed by the cationic surfactant, methyltrioctylammonium chloride (MTOAC). The best extraction efficiency of HW6 was obtained under the following conditions: pH 11.0, ionic strength 5.0 mM of KCl and micelle concentration 7.0 mM of MTOAC. The limits of detection (LODs) obtained for HW6 in water, urine and plasma samples were 0.15, 0.19 and 0.28 microM, respectively, with relative standard deviation (RSD) values in the range +/-8.8-10.5%. The sensitivity obtained in water by the present method was 45-fold higher than that of the conventional use of atmospheric-pressure (AP)-MALDI MS. Furthermore, the applicability of the proposed approach was extended for the determination of thiol-containing amino acids in sample solutions by using MALDI time-of-flight (TOF) MS. SN - 0951-4198 UR - https://www.unboundmedicine.com/medline/citation/18395891/Reverse_micellar_microextraction_for_rapid_analysis_of_thiol_containing_peptides_and_amino_acids_by_atmospheric_pressure_matrix_assisted_laser_desorption/ionization_ion_trap_and_matrix_assisted_laser_desorption/ionization_time_of_flight_mass_spectrometry_ DB - PRIME DP - Unbound Medicine ER -