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Brain-derived neurotrophic factor-mediated retrograde signaling required for the induction of long-term potentiation at inhibitory synapses of visual cortical pyramidal neurons.
Neurosci Res 2008; 61(2):192-200NR

Abstract

High-frequency stimulation (HFS) induces long-term potentiation (LTP) at inhibitory synapses of layer 5 pyramidal neurons in developing rat visual cortex. This LTP requires postsynaptic Ca2+ rise for induction, while the maintenance mechanism is present at the presynaptic site, suggesting presynaptic LTP expression and the necessity of retrograde signaling. We investigated whether the supposed signal is mediated by brain-derived neurotrophic factor (BDNF), which is expressed in pyramidal neurons but not inhibitory interneurons. LTP did not occur when HFS was applied in the presence of the Trk receptor tyrosine kinase inhibitor K252a in the perfusion medium. HFS produced LTP when bath application of K252a was started after HFS or when K252a was loaded into postsynaptic cells. LTP did not occur in the presence of TrkB-IgG scavenging BDNF or function-blocking anti-BDNF antibody in the medium. In cells loaded with the Ca2+ chelator BAPTA, the addition of BDNF to the medium enabled HFS to induce LTP without affecting baseline synaptic transmission. These results suggest that BDNF released from postsynaptic cells activates presynaptic TrkB, leading to LTP. Because BDNF, expressed activity dependently, regulates the maturation of cortical inhibition, inhibitory LTP may contribute to this developmental process, and hence experience-dependent functional maturation of visual cortex.

Authors+Show Affiliations

Department of Neuroscience, Research Institute of Environmental Medicine, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8601, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

18395922

Citation

Inagaki, Tsuyoshi, et al. "Brain-derived Neurotrophic Factor-mediated Retrograde Signaling Required for the Induction of Long-term Potentiation at Inhibitory Synapses of Visual Cortical Pyramidal Neurons." Neuroscience Research, vol. 61, no. 2, 2008, pp. 192-200.
Inagaki T, Begum T, Reza F, et al. Brain-derived neurotrophic factor-mediated retrograde signaling required for the induction of long-term potentiation at inhibitory synapses of visual cortical pyramidal neurons. Neurosci Res. 2008;61(2):192-200.
Inagaki, T., Begum, T., Reza, F., Horibe, S., Inaba, M., Yoshimura, Y., & Komatsu, Y. (2008). Brain-derived neurotrophic factor-mediated retrograde signaling required for the induction of long-term potentiation at inhibitory synapses of visual cortical pyramidal neurons. Neuroscience Research, 61(2), pp. 192-200. doi:10.1016/j.neures.2008.02.006.
Inagaki T, et al. Brain-derived Neurotrophic Factor-mediated Retrograde Signaling Required for the Induction of Long-term Potentiation at Inhibitory Synapses of Visual Cortical Pyramidal Neurons. Neurosci Res. 2008;61(2):192-200. PubMed PMID: 18395922.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Brain-derived neurotrophic factor-mediated retrograde signaling required for the induction of long-term potentiation at inhibitory synapses of visual cortical pyramidal neurons. AU - Inagaki,Tsuyoshi, AU - Begum,Tahamina, AU - Reza,Faruque, AU - Horibe,Shoko, AU - Inaba,Mie, AU - Yoshimura,Yumiko, AU - Komatsu,Yukio, Y1 - 2008/03/02/ PY - 2008/02/04/received PY - 2008/02/20/revised PY - 2008/02/25/accepted PY - 2008/4/9/pubmed PY - 2008/7/30/medline PY - 2008/4/9/entrez SP - 192 EP - 200 JF - Neuroscience research JO - Neurosci. Res. VL - 61 IS - 2 N2 - High-frequency stimulation (HFS) induces long-term potentiation (LTP) at inhibitory synapses of layer 5 pyramidal neurons in developing rat visual cortex. This LTP requires postsynaptic Ca2+ rise for induction, while the maintenance mechanism is present at the presynaptic site, suggesting presynaptic LTP expression and the necessity of retrograde signaling. We investigated whether the supposed signal is mediated by brain-derived neurotrophic factor (BDNF), which is expressed in pyramidal neurons but not inhibitory interneurons. LTP did not occur when HFS was applied in the presence of the Trk receptor tyrosine kinase inhibitor K252a in the perfusion medium. HFS produced LTP when bath application of K252a was started after HFS or when K252a was loaded into postsynaptic cells. LTP did not occur in the presence of TrkB-IgG scavenging BDNF or function-blocking anti-BDNF antibody in the medium. In cells loaded with the Ca2+ chelator BAPTA, the addition of BDNF to the medium enabled HFS to induce LTP without affecting baseline synaptic transmission. These results suggest that BDNF released from postsynaptic cells activates presynaptic TrkB, leading to LTP. Because BDNF, expressed activity dependently, regulates the maturation of cortical inhibition, inhibitory LTP may contribute to this developmental process, and hence experience-dependent functional maturation of visual cortex. SN - 0168-0102 UR - https://www.unboundmedicine.com/medline/citation/18395922/Brain_derived_neurotrophic_factor_mediated_retrograde_signaling_required_for_the_induction_of_long_term_potentiation_at_inhibitory_synapses_of_visual_cortical_pyramidal_neurons_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0168-0102(08)00075-8 DB - PRIME DP - Unbound Medicine ER -