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RETRACTED ARTICLE

beta-Catenin promotes self-renewal of skeletal-muscle satellite cells.
J Cell Sci. 2008 May 01; 121(Pt 9):1373-82.JC

Abstract

Satellite cells are the resident stem cells of adult skeletal muscle. As with all stem cells, how the choice between self-renewal or differentiation is controlled is central to understanding their function. Here, we have explored the role of beta-catenin in determining the fate of myogenic satellite cells. Satellite cells express beta-catenin, and expression is maintained as they activate and undergo proliferation. Constitutive retroviral-driven expression of wild-type or stabilised beta-catenin results in more satellite cells expressing Pax7 without any MyoD -- therefore, adopting the self-renewal pathway, with fewer cells undergoing myogenic differentiation. Similarly, preventing the degradation of endogenous beta-catenin by inhibiting GSK3beta activity also results in more Pax7-positive-MyoD-negative (Pax7(+)MyoD(-)) satellite-cell progeny. Consistent with these observations, downregulation of beta-catenin using small interfering RNA (siRNA) reduced the proportion of satellite cells that express Pax7 and augmented myogenic differentiation after mitogen withdrawal. Since a dominant-negative version of beta-catenin had the same effect as silencing beta-catenin using specific siRNA, beta-catenin promotes self-renewal via transcriptional control of target genes. Thus, beta-catenin signalling in proliferating satellite cells directs these cells towards the self-renewal pathway and, so, contributes to the maintenance of this stem-cell pool in adult skeletal muscle.

Authors+Show Affiliations

King's College London, Randall Division of Cell and Molecular Biophysics, New Hunt's House, Guy's Campus, London, UK.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Retracted Publication

Language

eng

PubMed ID

18397993

Citation

Perez-Ruiz, Ana, et al. "Beta-Catenin Promotes Self-renewal of Skeletal-muscle Satellite Cells." Journal of Cell Science, vol. 121, no. Pt 9, 2008, pp. 1373-82.
Perez-Ruiz A, Ono Y, Gnocchi VF, et al. Beta-Catenin promotes self-renewal of skeletal-muscle satellite cells. J Cell Sci. 2008;121(Pt 9):1373-82.
Perez-Ruiz, A., Ono, Y., Gnocchi, V. F., & Zammit, P. S. (2008). Beta-Catenin promotes self-renewal of skeletal-muscle satellite cells. Journal of Cell Science, 121(Pt 9), 1373-82. https://doi.org/10.1242/jcs.024885
Perez-Ruiz A, et al. Beta-Catenin Promotes Self-renewal of Skeletal-muscle Satellite Cells. J Cell Sci. 2008 May 1;121(Pt 9):1373-82. PubMed PMID: 18397993.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - beta-Catenin promotes self-renewal of skeletal-muscle satellite cells. AU - Perez-Ruiz,Ana, AU - Ono,Yusuke, AU - Gnocchi,Viola F, AU - Zammit,Peter S, Y1 - 2008/04/08/ PY - 2008/4/10/pubmed PY - 2008/8/23/medline PY - 2008/4/10/entrez SP - 1373 EP - 82 JF - Journal of cell science JO - J Cell Sci VL - 121 IS - Pt 9 N2 - Satellite cells are the resident stem cells of adult skeletal muscle. As with all stem cells, how the choice between self-renewal or differentiation is controlled is central to understanding their function. Here, we have explored the role of beta-catenin in determining the fate of myogenic satellite cells. Satellite cells express beta-catenin, and expression is maintained as they activate and undergo proliferation. Constitutive retroviral-driven expression of wild-type or stabilised beta-catenin results in more satellite cells expressing Pax7 without any MyoD -- therefore, adopting the self-renewal pathway, with fewer cells undergoing myogenic differentiation. Similarly, preventing the degradation of endogenous beta-catenin by inhibiting GSK3beta activity also results in more Pax7-positive-MyoD-negative (Pax7(+)MyoD(-)) satellite-cell progeny. Consistent with these observations, downregulation of beta-catenin using small interfering RNA (siRNA) reduced the proportion of satellite cells that express Pax7 and augmented myogenic differentiation after mitogen withdrawal. Since a dominant-negative version of beta-catenin had the same effect as silencing beta-catenin using specific siRNA, beta-catenin promotes self-renewal via transcriptional control of target genes. Thus, beta-catenin signalling in proliferating satellite cells directs these cells towards the self-renewal pathway and, so, contributes to the maintenance of this stem-cell pool in adult skeletal muscle. SN - 0021-9533 UR - https://www.unboundmedicine.com/medline/citation/18397993/beta_Catenin_promotes_self_renewal_of_skeletal_muscle_satellite_cells_ L2 - http://jcs.biologists.org/cgi/pmidlookup?view=long&pmid=18397993 DB - PRIME DP - Unbound Medicine ER -