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[The establishment and assessment on cosmetic products using 3T3 mouse fibroblast neutral red uptake phototoxicity assay].
Zhonghua Yu Fang Yi Xue Za Zhi 2007; 41(6):479-82ZY

Abstract

OBJECTIVE

To establish the 3T3 mouse fibroblast neutral red uptake (NRU-PT) phototoxicity test method, and evaluate the practicality of the method in detecting potential phototoxicity of the cosmetic products.

METHODS

Fifteen phototoxic and 9 non-phototoxic chemicals were tested in our laboratories, the phototoxic potential of the test chemicals was evaluated in a prediction model in which either the photo irritation factor (PIF) or the mean photo effect (MPE) was compared with the coherence and sensitivity of the method. 20 kinds of functional cosmetics were detected and the results were analyzed by the 3T3 NRU-PT in vitro and Guinea pig skin phototoxicity test (in vivo).

RESULTS

Both PIF and MPE of the chemicals were highly reproduced, and the correlation between in vitro and in vivo data was almost perfect. All the non-phototoxic provided a negative result, while 14 of the 15 phototoxic tested chemicals gave clear positive results. For cosmetics, the correlation between in vitro and in vivo data was consistent.

CONCLUSION

The 3T3 NRU PT test was established successfully, it should be used as a good alternative method for assessing the phototoxic potential of the chemicals and cosmetics in China.

Authors+Show Affiliations

Center for Disease Control and Prevention of Guangdong Province, Guangzhou 510030, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

18399129

Citation

Yang, Ying, et al. "[The Establishment and Assessment On Cosmetic Products Using 3T3 Mouse Fibroblast Neutral Red Uptake Phototoxicity Assay]." Zhonghua Yu Fang Yi Xue Za Zhi [Chinese Journal of Preventive Medicine], vol. 41, no. 6, 2007, pp. 479-82.
Yang Y, Xiong XK, Yang XF, et al. [The establishment and assessment on cosmetic products using 3T3 mouse fibroblast neutral red uptake phototoxicity assay]. Zhonghua Yu Fang Yi Xue Za Zhi. 2007;41(6):479-82.
Yang, Y., Xiong, X. K., Yang, X. F., He, X. W., Huang, J. M., Tan, X. H., & Zheng, S. S. (2007). [The establishment and assessment on cosmetic products using 3T3 mouse fibroblast neutral red uptake phototoxicity assay]. Zhonghua Yu Fang Yi Xue Za Zhi [Chinese Journal of Preventive Medicine], 41(6), pp. 479-82.
Yang Y, et al. [The Establishment and Assessment On Cosmetic Products Using 3T3 Mouse Fibroblast Neutral Red Uptake Phototoxicity Assay]. Zhonghua Yu Fang Yi Xue Za Zhi. 2007;41(6):479-82. PubMed PMID: 18399129.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [The establishment and assessment on cosmetic products using 3T3 mouse fibroblast neutral red uptake phototoxicity assay]. AU - Yang,Ying, AU - Xiong,Xi-Kun, AU - Yang,Xing-Fen, AU - He,Xi-Wen, AU - Huang,Jun-Ming, AU - Tan,Xiao-Hua, AU - Zheng,Sui-Sheng, PY - 2008/4/11/pubmed PY - 2009/5/15/medline PY - 2008/4/11/entrez SP - 479 EP - 82 JF - Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine] JO - Zhonghua Yu Fang Yi Xue Za Zhi VL - 41 IS - 6 N2 - OBJECTIVE: To establish the 3T3 mouse fibroblast neutral red uptake (NRU-PT) phototoxicity test method, and evaluate the practicality of the method in detecting potential phototoxicity of the cosmetic products. METHODS: Fifteen phototoxic and 9 non-phototoxic chemicals were tested in our laboratories, the phototoxic potential of the test chemicals was evaluated in a prediction model in which either the photo irritation factor (PIF) or the mean photo effect (MPE) was compared with the coherence and sensitivity of the method. 20 kinds of functional cosmetics were detected and the results were analyzed by the 3T3 NRU-PT in vitro and Guinea pig skin phototoxicity test (in vivo). RESULTS: Both PIF and MPE of the chemicals were highly reproduced, and the correlation between in vitro and in vivo data was almost perfect. All the non-phototoxic provided a negative result, while 14 of the 15 phototoxic tested chemicals gave clear positive results. For cosmetics, the correlation between in vitro and in vivo data was consistent. CONCLUSION: The 3T3 NRU PT test was established successfully, it should be used as a good alternative method for assessing the phototoxic potential of the chemicals and cosmetics in China. SN - 0253-9624 UR - https://www.unboundmedicine.com/medline/citation/18399129/[The_establishment_and_assessment_on_cosmetic_products_using_3T3_mouse_fibroblast_neutral_red_uptake_phototoxicity_assay]_ L2 - http://journal.yiigle.com/LinkIn.do?linkin_type=pubmed&issn=0253-9624&year=2007&vol=41&issue=6&fpage=479 DB - PRIME DP - Unbound Medicine ER -